Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DBAC_RS05745 Genome accession   NC_013173
Coordinates   1237535..1238617 (+) Length   360 a.a.
NCBI ID   WP_015773333.1    Uniprot ID   C7LR89
Organism   Desulfomicrobium baculatum DSM 4028     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1232535..1243617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBAC_RS05715 (Dbac_1125) mgtE 1232639..1233940 (+) 1302 WP_228644961.1 magnesium transporter -
  DBAC_RS05720 (Dbac_1126) - 1233953..1234384 (-) 432 WP_015773328.1 CBS domain-containing protein -
  DBAC_RS05725 (Dbac_1127) - 1234537..1235208 (+) 672 WP_015773329.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  DBAC_RS05730 (Dbac_1128) - 1235324..1236232 (+) 909 WP_015773330.1 Mrp/NBP35 family ATP-binding protein -
  DBAC_RS05735 (Dbac_1129) - 1236252..1236854 (+) 603 WP_015773331.1 lytic transglycosylase domain-containing protein -
  DBAC_RS05740 (Dbac_1130) pgsA 1236861..1237400 (+) 540 WP_015773332.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  DBAC_RS05745 (Dbac_1131) pilT 1237535..1238617 (+) 1083 WP_015773333.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DBAC_RS05750 (Dbac_1132) - 1238619..1239794 (+) 1176 WP_015773334.1 PilT/PilU family type 4a pilus ATPase -
  DBAC_RS05755 (Dbac_1133) - 1239804..1240388 (+) 585 WP_015773335.1 zinc-ribbon domain-containing protein -
  DBAC_RS20030 - 1240392..1240673 (+) 282 WP_043810484.1 septum formation initiator family protein -
  DBAC_RS05765 (Dbac_1134) - 1240675..1241427 (+) 753 WP_015773336.1 tetratricopeptide repeat protein -
  DBAC_RS05770 (Dbac_1135) - 1241560..1242252 (+) 693 WP_015773337.1 hypothetical protein -
  DBAC_RS05775 (Dbac_1136) fbp 1242258..1243274 (+) 1017 WP_015773338.1 class 1 fructose-bisphosphatase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40297.63 Da        Isoelectric Point: 6.5976

>NTDB_id=35123 DBAC_RS05745 WP_015773333.1 1237535..1238617(+) (pilT) [Desulfomicrobium baculatum DSM 4028]
MAQIDAFFKMMHELGASDLHLSSGSQPIIRLHGEMQRIKYKFLEHEELKKMLYEITPENKIKTFEESGDVDFSYEVTHLA
RYRANFFLQKRGIGAVFREIPQKILTIEELGLPSILKNLALLPKGLVLVTGPTGSGKSTTLAAIVDYVNKIRKDHILTIE
DPIEFVHEPQSCLINQREVGRDTMSFSAALRGALREDPDIILVGEMRDLETIQLALEAAETGHLVFATLHTISASKTIDR
IIEVFPGDLQGQIRSGLADSLRAIIAQNLFKRIDRPGRVAGIEVLIATPAVRNLIRENKIFQINSVIETGRKFGMQSIDD
AIMKLLTAGVIDAEQAYNKAATKSKFREFLTTPPQDFTEV

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=35123 DBAC_RS05745 WP_015773333.1 1237535..1238617(+) (pilT) [Desulfomicrobium baculatum DSM 4028]
ATGGCTCAAATAGATGCCTTTTTTAAAATGATGCATGAGCTTGGGGCCTCGGATCTTCATCTGTCATCAGGTTCGCAGCC
GATTATCCGTCTGCATGGTGAAATGCAGCGCATCAAATACAAATTTCTTGAGCATGAAGAACTCAAGAAAATGCTCTACG
AAATAACTCCAGAAAATAAGATCAAGACATTCGAAGAAAGCGGTGATGTGGACTTCTCCTACGAAGTCACTCATTTGGCT
CGGTACCGCGCCAATTTCTTTTTGCAGAAGCGTGGAATCGGGGCGGTTTTTCGAGAAATTCCGCAAAAAATCCTTACCAT
CGAGGAACTGGGCCTGCCGAGCATCCTGAAGAATCTGGCTTTATTGCCCAAGGGACTGGTATTGGTCACCGGCCCTACGG
GTAGCGGTAAATCCACCACTCTGGCCGCCATTGTCGATTACGTGAACAAGATCCGCAAGGATCATATCCTGACCATCGAA
GACCCCATCGAATTCGTACACGAACCGCAGAGCTGCCTGATCAACCAGCGTGAAGTCGGCCGGGACACCATGTCTTTCAG
CGCCGCCTTGCGCGGGGCGCTACGCGAGGACCCGGACATCATTCTGGTCGGCGAAATGCGAGACCTCGAAACCATTCAGC
TGGCTCTTGAGGCGGCCGAGACCGGCCATCTGGTTTTTGCGACCCTGCATACCATCTCCGCCTCGAAAACCATCGACCGC
ATCATCGAGGTCTTTCCCGGTGATTTACAGGGTCAGATTCGCTCCGGCCTTGCCGACTCCCTGCGGGCCATCATTGCGCA
GAACCTTTTCAAGCGCATAGACAGACCCGGGCGCGTTGCCGGCATTGAAGTCCTCATCGCCACCCCTGCCGTGCGAAACC
TGATCCGCGAAAACAAGATTTTTCAGATCAACTCCGTTATCGAGACCGGACGAAAGTTCGGCATGCAGAGCATCGACGAC
GCCATCATGAAGCTTCTCACCGCCGGTGTCATCGACGCTGAGCAAGCATACAACAAGGCTGCCACGAAGTCGAAATTCAG
GGAATTTCTCACCACTCCGCCCCAAGATTTCACCGAGGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C7LR89

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

58.133

92.222

0.536
  pilT Acinetobacter baylyi ADP1

57.704

91.944

0.531
  pilT Pseudomonas stutzeri DSM 10701

57.229

92.222

0.528
  pilT Acinetobacter nosocomialis M2

57.229

92.222

0.528
  pilT Acinetobacter baumannii D1279779

57.229

92.222

0.528
  pilT Acinetobacter baumannii strain A118

57.229

92.222

0.528
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.024

92.222

0.517
  pilT Vibrio cholerae strain A1552

56.024

92.222

0.517
  pilT Neisseria meningitidis 8013

56.923

90.278

0.514
  pilT Legionella pneumophila strain Lp02

55.891

91.944

0.514
  pilT Legionella pneumophila strain ERS1305867

55.891

91.944

0.514
  pilT Neisseria gonorrhoeae MS11

56.615

90.278

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.105

95.278

0.458
  pilU Pseudomonas stutzeri DSM 10701

40.29

95.833

0.386
  pilU Vibrio cholerae strain A1552

37.571

98.333

0.369
  pilU Acinetobacter baylyi ADP1

38.596

95

0.367

Multiple sequence alignment