Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E2R62_RS21555 Genome accession   NZ_CP038008
Coordinates   4495789..4496769 (+) Length   326 a.a.
NCBI ID   WP_012908851.1    Uniprot ID   D2TSY7
Organism   Citrobacter rodentium strain DBS100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4490789..4501769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2R62_RS21525 (E2R62_21920) ansB 4491308..4492354 (+) 1047 WP_012908845.1 L-asparaginase 2 -
  E2R62_RS21530 (E2R62_21925) hemW 4492465..4493601 (-) 1137 WP_012908846.1 radical SAM family heme chaperone HemW -
  E2R62_RS21535 (E2R62_21930) - 4493594..4494187 (-) 594 WP_012908847.1 XTP/dITP diphosphatase -
  E2R62_RS21540 (E2R62_21935) yggU 4494195..4494485 (-) 291 WP_012908848.1 DUF167 family protein YggU -
  E2R62_RS21545 (E2R62_21940) - 4494482..4495048 (-) 567 WP_012908849.1 YggT family protein -
  E2R62_RS21550 (E2R62_21945) - 4495067..4495771 (-) 705 WP_012908850.1 YggS family pyridoxal phosphate-dependent enzyme -
  E2R62_RS21555 (E2R62_21950) pilT 4495789..4496769 (+) 981 WP_012908851.1 type IV pilus twitching motility protein PilT Machinery gene
  E2R62_RS21560 (E2R62_21955) ruvX 4496805..4497221 (-) 417 WP_024133119.1 Holliday junction resolvase RuvX -
  E2R62_RS21565 (E2R62_21960) - 4497221..4497784 (-) 564 WP_012908853.1 YqgE/AlgH family protein -
  E2R62_RS21570 (E2R62_21965) gshB 4497974..4498921 (-) 948 WP_012908854.1 glutathione synthase -
  E2R62_RS21575 (E2R62_21970) rsmE 4498934..4499665 (-) 732 WP_012908855.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E2R62_RS21580 (E2R62_21975) endA 4499739..4500446 (-) 708 WP_012908856.1 deoxyribonuclease I -
  E2R62_RS21585 (E2R62_21980) - 4500538..4501038 (-) 501 WP_012908857.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35908.12 Da        Isoelectric Point: 7.1514

>NTDB_id=351219 E2R62_RS21555 WP_012908851.1 4495789..4496769(+) (pilT) [Citrobacter rodentium strain DBS100]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRLEAAPFSAPDVNELLSQWLDETQRAALRQEGQLDFAVSLTANQRLRA
SAFRQLQGMSLALRLLPNQCPTLDALDVPAALPELLTCENGLLLVSGATGSGKSTTLAAMVEWLNRHRDGHILTLEDPIE
YRYASQRCLIQQREVGVHCSSFVAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPLRSQLAGSLRAVLAQKLVPDKQEGRVALFELLINTPAAGNLIREGKTYQLPHVIQTGQQTGMMTFAQSVQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=351219 E2R62_RS21555 WP_012908851.1 4495789..4496769(+) (pilT) [Citrobacter rodentium strain DBS100]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGAGCAATGCGTGGCCAGCTCG
CTGGCGCAAGCGGGGAAGGCTGGAAGCTGCGCCATTTTCCGCGCCGGATGTAAACGAACTCCTGTCGCAGTGGCTTGATG
AGACGCAGCGAGCCGCGCTACGCCAGGAGGGCCAGCTTGATTTCGCCGTATCGCTTACCGCCAATCAGCGTCTGCGGGCC
AGCGCCTTTCGCCAGCTGCAGGGGATGTCGCTGGCGCTGCGGCTGTTGCCGAATCAGTGCCCAACGCTTGACGCGCTGGA
TGTGCCTGCGGCGTTGCCGGAACTGCTGACCTGCGAGAACGGCCTGCTGCTGGTCTCCGGCGCGACCGGCAGCGGCAAAT
CAACCACCCTGGCGGCGATGGTGGAGTGGCTGAACCGACATCGCGACGGCCATATCCTGACCCTGGAAGATCCGATTGAA
TATCGCTACGCCAGCCAGCGCTGTCTGATTCAGCAACGGGAAGTGGGCGTACACTGTTCCTCGTTTGTGGCGGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATCCTGTTGGGGGAATTGCGTGATAGCGAAACCATCCGCCTGGCGCTGACGG
CGGCGGAGACGGGGCATCTGGTGCTGGCTACGCTGCATACCCGCGGCGCGGCGCAGGCTATCGAGCGGCTGGTTGATACC
TTTCCGGCGCAGGAAAAAGATCCGCTGCGTAGTCAACTGGCGGGCAGCCTGCGCGCCGTGCTGGCGCAAAAACTGGTGCC
GGATAAACAGGAGGGGCGCGTTGCGCTGTTCGAACTGTTAATCAACACGCCCGCTGCGGGGAATCTGATCCGCGAGGGCA
AAACGTACCAGTTACCGCATGTGATCCAGACCGGGCAGCAGACGGGCATGATGACTTTCGCCCAGAGCGTGCAGCAGCGC
CAGGCGCAGGGGCGATTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2TSY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Neisseria meningitidis 8013

45.732

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Neisseria gonorrhoeae MS11

45.427

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

45.342

98.773

0.448
  pilT Legionella pneumophila strain Lp02

45.342

98.773

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Acinetobacter baylyi ADP1

37.538

99.693

0.374

Multiple sequence alignment