Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E1B03_RS22000 Genome accession   NZ_CP037864
Coordinates   4370657..4371637 (-) Length   326 a.a.
NCBI ID   WP_133086922.1    Uniprot ID   -
Organism   Citrobacter arsenatis strain LY-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4365657..4376637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1B03_RS21970 (E1B03_21975) - 4366432..4366929 (+) 498 WP_103769033.1 SprT family zinc-dependent metalloprotease -
  E1B03_RS21975 (E1B03_21980) endA 4367024..4367731 (+) 708 WP_133086919.1 deoxyribonuclease I -
  E1B03_RS21980 (E1B03_21985) rsmE 4367806..4368537 (+) 732 WP_133086920.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E1B03_RS21985 (E1B03_21990) gshB 4368557..4369504 (+) 948 WP_103769030.1 glutathione synthase -
  E1B03_RS21990 (E1B03_21995) - 4369681..4370244 (+) 564 WP_003027086.1 YqgE/AlgH family protein -
  E1B03_RS21995 (E1B03_22000) ruvX 4370244..4370660 (+) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  E1B03_RS22000 (E1B03_22005) pilT 4370657..4371637 (-) 981 WP_133086922.1 type IV pilus twitching motility protein PilT Machinery gene
  E1B03_RS22005 (E1B03_22010) - 4371655..4372359 (+) 705 WP_075145648.1 YggS family pyridoxal phosphate-dependent enzyme -
  E1B03_RS22010 (E1B03_22015) - 4372378..4372944 (+) 567 WP_087051206.1 YggT family protein -
  E1B03_RS22015 (E1B03_22020) yggU 4372941..4373231 (+) 291 WP_005123286.1 DUF167 family protein YggU -
  E1B03_RS22020 (E1B03_22025) - 4373239..4373832 (+) 594 WP_103769027.1 XTP/dITP diphosphatase -
  E1B03_RS22025 (E1B03_22030) hemW 4373825..4374961 (+) 1137 WP_103769026.1 radical SAM family heme chaperone HemW -
  E1B03_RS22030 (E1B03_22035) ansB 4375080..4376126 (-) 1047 WP_133086923.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36023.24 Da        Isoelectric Point: 6.4948

>NTDB_id=350282 E1B03_RS22000 WP_133086922.1 4370657..4371637(-) (pilT) [Citrobacter arsenatis strain LY-1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIHGKVEIAPFTPPDVEDLLMCWLSEQQQVQWREQGQIDFAITLACSRRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPGVIPELLHCEHGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FTAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=350282 E1B03_RS22000 WP_133086922.1 4370657..4371637(-) (pilT) [Citrobacter arsenatis strain LY-1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACATGGAAAAGTCGAAATCGCGCCATTTACTCCGCCTGACGTGGAGGATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAGCAGGGGCAGATTGATTTTGCTATTACGCTGGCATGTTCCCGGCGTCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACGTCGCTGGCGCTAAGATTGCTACCACTTGATTGTCCACGTTTAGACGATCTTCA
GACTCCCGGGGTCATACCTGAACTGTTGCACTGTGAACATGGATTGATTCTGGTCACGGGTGCCACCGGCAGCGGTAAGT
CAACGACCCTGGCGGCGATGGTGGAGTACCTTAATCAGCATGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAGCAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCTGGTTTGCG
CGGCGCACTGCGTGAAGATCCTGACGTCATTTTGCTGGGCGAACTGCGTGACGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACAGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCTATCGCGCGGCTGGTAGACACC
TTTACCGCACAGGAGAAAGATCCGGTACGGAACCAACTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGGCGTGTGGCGTTATTCGAGCTGCTCGTCAACACGCCTGCCGTGGGTAATTTGATTCGTGAAGGTA
AAACACATCAGCTACCCGGTGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

45.732

100

0.46
  pilT Neisseria gonorrhoeae MS11

45.427

100

0.457
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368

Multiple sequence alignment