Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EYZ66_RS13480 Genome accession   NZ_CP036423
Coordinates   2943111..2944145 (-) Length   344 a.a.
NCBI ID   WP_009576350.1    Uniprot ID   F3L3I1
Organism   Aequoribacter fuscus strain IMCC3088     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2938111..2949145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYZ66_RS13450 (EYZ66_13455) metF 2938705..2939556 (+) 852 WP_009576344.1 methylenetetrahydrofolate reductase [NAD(P)H] -
  EYZ66_RS13455 (EYZ66_13460) - 2939546..2940277 (+) 732 WP_040816859.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EYZ66_RS13460 (EYZ66_13465) gshB 2940300..2941250 (+) 951 WP_009576346.1 glutathione synthase -
  EYZ66_RS13465 (EYZ66_13470) - 2941253..2942071 (+) 819 WP_009576347.1 TonB family protein -
  EYZ66_RS13470 (EYZ66_13475) - 2942133..2942693 (+) 561 WP_009576348.1 YqgE/AlgH family protein -
  EYZ66_RS13475 (EYZ66_13480) ruvX 2942695..2943114 (+) 420 WP_009576349.1 Holliday junction resolvase RuvX -
  EYZ66_RS13480 (EYZ66_13485) pilT 2943111..2944145 (-) 1035 WP_009576350.1 type IV pilus twitching motility protein PilT Machinery gene
  EYZ66_RS13485 (EYZ66_13490) - 2944231..2944926 (+) 696 WP_009576351.1 YggS family pyridoxal phosphate-dependent enzyme -
  EYZ66_RS13490 (EYZ66_13495) proC 2944972..2945799 (+) 828 WP_009576352.1 pyrroline-5-carboxylate reductase -
  EYZ66_RS13495 (EYZ66_13500) - 2945799..2946386 (+) 588 WP_009576353.1 YggT family protein -
  EYZ66_RS13500 (EYZ66_13505) - 2946387..2947529 (+) 1143 WP_009576354.1 homoserine O-acetyltransferase -
  EYZ66_RS13505 (EYZ66_13510) metW 2947526..2948107 (+) 582 WP_009576355.1 methionine biosynthesis protein MetW -
  EYZ66_RS13510 (EYZ66_13515) - 2948113..2948553 (+) 441 WP_040816860.1 DUF4426 domain-containing protein -
  EYZ66_RS13515 (EYZ66_13520) rdgB 2948546..2949136 (+) 591 WP_009576357.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38347.12 Da        Isoelectric Point: 5.8996

>NTDB_id=348854 EYZ66_RS13480 WP_009576350.1 2943111..2944145(-) (pilT) [Aequoribacter fuscus strain IMCC3088]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVQELVYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGCGAVFRTIPSKVLTMEDLGMGKVFRDISMTPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHILTIED
PIEFVHDSKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAAEKSMVRSMLSESLQAVISQTLLKRAGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGQAIGMQTMDQCL
MDLVDERIITKDIAREKARSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=348854 EYZ66_RS13480 WP_009576350.1 2943111..2944145(-) (pilT) [Aequoribacter fuscus strain IMCC3088]
TTGGATATCACAGAATTGCTAGCCTTTAGCGCTAAGCAAAAAGCCTCTGACCTGCACCTATCGGCAGGTCTGCCGCCCAT
GATTCGTGTTGACGGAGACGTGCGTCGTATTAATTTGCCTGCGCTGGATCATAAAGAAGTGCAGGAGCTTGTCTACGATA
TTATGAATGACAAGCAGCGCAAAGATTTCGAGGAGTTTTTAGAGACGGACTTCTCGTTTGAAGTCCCGGGTGTTGCGCGT
TTCAGGGTCAACGCCTTTAACCAGAATCGGGGCTGTGGCGCGGTATTTAGAACCATTCCTTCCAAAGTGCTGACCATGGA
AGACTTGGGGATGGGCAAAGTATTTCGGGATATCTCGATGACGCCGCGCGGTCTGGTGCTTGTCACAGGGCCGACAGGCT
CGGGTAAATCGACTACCTTGGCCGCCATGATTGACTACATCAACGACAATAAATACGAGCACATCCTGACCATTGAAGAC
CCAATCGAATTCGTACACGACAGCAAGAAATGCTTGGTCAACCAGCGTGAGGTACACCGTGATACGTTGGGTTTTGCCGA
AGCTTTACGCTCGGCGCTACGCGAAGACCCCGACATTATTCTGGTGGGCGAGATGCGCGACCTCGAAACCATCCGTCTGG
CTTTAACGGCAGCTGAGACGGGCCACTTAGTGTTTGGCACCTTACACACCACCTCGGCGGCCAAAACTATCGACCGTATC
GTGGACGTATTCCCCGCGGCCGAAAAGTCTATGGTTCGTTCTATGCTGTCAGAATCGTTACAGGCGGTGATTTCGCAAAC
GCTACTCAAGCGCGCAGGCGGTGGGCGCGTAGCGGCACACGAAATCATGCGTGGTACACCGGCCATTCGAAACCTCATTC
GTGAGGACAAAATTGCACAGATGTATTCGGCGATTCAAACCGGTCAGGCCATTGGCATGCAAACCATGGATCAGTGTTTG
ATGGACTTAGTGGACGAACGCATTATTACCAAAGACATCGCTCGCGAAAAGGCTCGTTCACCCGAGAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F3L3I1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.919

100

0.869
  pilT Pseudomonas aeruginosa PAK

86.047

100

0.86
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

81.686

100

0.817
  pilT Acinetobacter baumannii strain A118

81.686

100

0.817
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

75

100

0.75
  pilT Legionella pneumophila strain ERS1305867

75

100

0.75
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.64

0.666
  pilT Vibrio cholerae strain A1552

69.605

95.64

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.913

100

0.541
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

41.617

97.093

0.404

Multiple sequence alignment