Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Pan97_RS16690 Genome accession   NZ_CP036289
Coordinates   4228810..4229910 (+) Length   366 a.a.
NCBI ID   WP_144974475.1    Uniprot ID   A0A518CAY5
Organism   Bremerella volcania strain Pan97     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4223810..4234910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pan97_RS16670 (Pan97_34400) - 4224125..4224592 (+) 468 WP_144974467.1 hypothetical protein -
  Pan97_RS16675 (Pan97_34410) - 4224772..4225626 (-) 855 WP_144974468.1 outer membrane beta-barrel protein -
  Pan97_RS16680 (Pan97_34420) - 4225799..4226473 (+) 675 WP_144974470.1 hypothetical protein -
  Pan97_RS16685 (Pan97_34430) - 4226739..4228463 (+) 1725 WP_144974473.1 GspE/PulE family protein -
  Pan97_RS16690 (Pan97_34440) pilT 4228810..4229910 (+) 1101 WP_144974475.1 type IV pilus twitching motility protein PilT Machinery gene
  Pan97_RS16695 (Pan97_34450) - 4229971..4231695 (+) 1725 WP_144974477.1 GspE/PulE family protein -
  Pan97_RS16700 (Pan97_34460) - 4231768..4233159 (+) 1392 WP_144974479.1 type II secretion system F family protein -
  Pan97_RS16705 (Pan97_34470) - 4233321..4234262 (+) 942 WP_144974481.1 type II secretion system protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40288.98 Da        Isoelectric Point: 6.9270

>NTDB_id=347874 Pan97_RS16690 WP_144974475.1 4228810..4229910(+) (pilT) [Bremerella volcania strain Pan97]
MATILIDKLLQAAVKQGASDIHITVGQPPVFRLHGRMRQLDTKSLEPDDTVSLMKSITPERCQRELQESGGSDFGFAFSD
LARFRVSIFRQRGNISMVLRQIPNDMLTPEQLGLPAKVLELCHRPRGLFLVTGPTGSGKSTTLASVINHLNETVDHHIIT
IEDPIEFYHYHKKSTVNQREIGVDVPSFSEAIRRALRQDPDVILVGEMRDLETIEAAISAAETGHVVFGTLHTNSAAGTV
DRIIDVFPSGQQDQIRTQLGSALLGVLAQTLCPKIGGGRCAAYELLNVTSGISNLIRENKTFRIPSMIQTGSKHGMILMD
DSLFNHWRADRITMEDALAKAQNPNDLAKRIADARRGNVDDREGAA

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=347874 Pan97_RS16690 WP_144974475.1 4228810..4229910(+) (pilT) [Bremerella volcania strain Pan97]
ATGGCGACGATTCTTATTGACAAGTTGTTGCAGGCTGCTGTGAAGCAGGGCGCAAGCGATATCCATATCACGGTGGGTCA
GCCGCCGGTGTTTCGTTTGCACGGTCGCATGCGACAGCTCGATACGAAATCGCTGGAACCAGACGATACCGTTTCGCTGA
TGAAGAGTATTACGCCCGAACGCTGTCAACGAGAGCTCCAGGAATCGGGGGGTTCCGACTTTGGTTTCGCGTTCTCCGAC
CTGGCCCGTTTCCGCGTGTCGATTTTCCGTCAGCGCGGAAACATCTCGATGGTGTTGCGGCAGATTCCCAATGACATGCT
CACCCCCGAACAGCTGGGCCTGCCGGCAAAAGTCCTCGAGCTTTGCCATCGGCCGCGCGGCTTGTTCCTGGTTACCGGTC
CGACGGGTTCTGGTAAGTCGACCACGTTGGCATCCGTGATCAACCACCTCAACGAAACCGTCGACCACCATATCATCACG
ATCGAAGACCCGATCGAATTTTATCACTACCACAAAAAGTCGACGGTCAATCAGCGCGAAATCGGCGTTGACGTCCCTTC
GTTCTCGGAAGCCATTCGTCGTGCGTTGCGCCAGGACCCCGACGTGATCCTCGTGGGTGAAATGCGTGACCTGGAAACCA
TTGAGGCTGCTATTTCCGCGGCGGAAACCGGGCACGTGGTGTTCGGGACGCTGCATACCAACAGTGCGGCAGGTACCGTC
GATCGTATCATCGACGTGTTCCCCTCCGGTCAGCAGGATCAGATCCGTACGCAGTTAGGTTCGGCCCTCTTGGGCGTGCT
CGCTCAAACGCTGTGCCCCAAGATCGGCGGTGGACGCTGTGCTGCTTACGAGCTTTTGAACGTCACCTCCGGTATTTCCA
ACCTGATTCGTGAAAACAAAACATTCCGTATTCCATCGATGATTCAGACCGGCTCGAAGCACGGGATGATTCTGATGGAC
GACTCCCTTTTTAATCACTGGCGGGCCGACCGCATCACCATGGAAGACGCGCTGGCTAAAGCCCAAAACCCCAATGACCT
GGCAAAGCGTATCGCCGACGCCCGACGCGGAAATGTCGACGATCGAGAAGGTGCTGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A518CAY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.235

97.814

0.511
  pilT Pseudomonas aeruginosa PAK

49.123

93.443

0.459
  pilT Acinetobacter baumannii strain A118

49.263

92.623

0.456
  pilT Acinetobacter nosocomialis M2

49.263

92.623

0.456
  pilT Acinetobacter baumannii D1279779

49.263

92.623

0.456
  pilT Acinetobacter baylyi ADP1

48.673

92.623

0.451
  pilT Pseudomonas stutzeri DSM 10701

47.953

93.443

0.448
  pilT Neisseria meningitidis 8013

46.512

93.989

0.437
  pilT Legionella pneumophila strain ERS1305867

50.314

86.885

0.437
  pilT Legionella pneumophila strain Lp02

50.314

86.885

0.437
  pilT Neisseria gonorrhoeae MS11

46.221

93.989

0.434
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.58

86.612

0.421
  pilT Vibrio cholerae strain A1552

48.58

86.612

0.421
  pilU Acinetobacter baylyi ADP1

38.95

98.907

0.385
  pilU Pseudomonas stutzeri DSM 10701

40.233

93.716

0.377
  pilU Vibrio cholerae strain A1552

37.571

96.721

0.363

Multiple sequence alignment