Detailed information NTG0034767

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMOL_RS04330
• Genome accession: NC_012968
• Coordinates: 924311..925414 (-)
• Length: 367 a.a.
• NCBI ID: WP_015831797.1
• Uniprot ID: C6WV12
• Organism: Methylotenera mobilis JLW8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 919311..930414

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMOL_RS04325 (Mmol_0850)	-	921662..924202 (+)	2541	WP_015831796.1	chemotaxis protein CheW	-
MMOL_RS04330 (Mmol_0851)	pilU	924311..925414 (-)	1104	WP_015831797.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MMOL_RS04335 (Mmol_0852)	-	925560..927665 (-)	2106	WP_015831798.1	tetratricopeptide repeat protein	-
MMOL_RS04340	mnmC	927675..928913 (-)	1239	WP_041928534.1	FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC	-
MMOL_RS04345	mnmD	928906..929658 (-)	753	WP_041928535.1	tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD	-

Sequence

Protein

• Length: 367 a.a.
• Molecular weight: 40426.52 Da
• Isoelectric Point: 5.6703

>NTDB_id=34767 MMOL_RS04330 WP_015831797.1 924311..925414(-) (pilU) [Methylotenera mobilis JLW8]
MAAIDITPVLKFMLDKGGSDLFFSSGTSIHIEVEGETTPINAQIMQPGMIKEIAYSLMSEDQIKEFEATLECNFALGKKD
IGRFRVNVFRQRGEVGMVIRHIKTDIPSLESLGLPPVLKDLISRKRGLILVVGATGSGKSTTLASMIDYRNETSNGHILT
LEDPIEFIHPHKKSVVDQREIGIDTLSFENGLKNAMRQAPDVILIGEVRDMEGMKNALAYAETGHLCLATLHANNANQAL
ERIISFFPEDGRNGLLLGISMNLVSIISQRLIPGVQSKRVAATEVMINTPYMNELIQKQKMGEIKEIMAENTDIGMHTFD
QSLFKLFSNGKIDDENALAYADSRNDLSLKIRFSAEGTRNSASGLGL

Nucleotide

• Length: 1104 bp

>NTDB_id=34767 MMOL_RS04330 WP_015831797.1 924311..925414(-) (pilU) [Methylotenera mobilis JLW8]
ATGGCTGCAATTGACATCACCCCTGTATTGAAATTTATGCTGGACAAAGGTGGTTCAGATTTATTCTTTAGCTCAGGCAC
CAGTATCCATATAGAGGTTGAAGGCGAAACCACGCCCATCAACGCCCAAATCATGCAGCCTGGCATGATTAAAGAAATTG
CTTACTCACTGATGAGTGAAGATCAGATTAAAGAGTTTGAAGCCACACTGGAGTGCAATTTTGCATTAGGTAAAAAAGAT
ATTGGCCGCTTCCGCGTGAATGTGTTCAGGCAACGCGGTGAAGTGGGTATGGTGATTCGGCATATCAAGACAGATATCCC
TTCATTAGAAAGCTTAGGCCTGCCGCCAGTGCTCAAGGATTTAATCTCACGCAAACGCGGGCTGATTTTGGTAGTGGGTG
CTACAGGCTCGGGTAAATCTACTACCTTAGCTTCAATGATTGATTACCGTAATGAAACCAGCAATGGTCATATACTCACG
CTGGAAGACCCTATCGAGTTTATTCACCCGCATAAGAAATCAGTAGTGGACCAGCGCGAAATCGGTATCGACACGCTATC
TTTCGAGAATGGCCTTAAAAATGCCATGCGCCAAGCCCCGGATGTGATTCTGATTGGTGAGGTACGCGATATGGAAGGTA
TGAAAAACGCACTAGCCTACGCAGAAACCGGGCATTTATGCTTAGCTACCTTACATGCTAATAATGCCAACCAAGCACTT
GAGCGCATTATTTCCTTCTTCCCAGAAGATGGCAGGAATGGATTGCTATTGGGAATCTCTATGAATCTGGTGAGCATCAT
ATCGCAGCGTTTAATCCCGGGGGTGCAAAGCAAACGTGTGGCCGCTACTGAGGTGATGATTAACACCCCCTATATGAACG
AGCTGATACAGAAGCAAAAAATGGGGGAAATCAAAGAAATCATGGCTGAGAATACCGATATCGGCATGCATACCTTTGAC
CAATCCCTATTCAAGCTCTTCTCTAATGGCAAAATTGACGATGAAAACGCGCTGGCTTATGCGGATTCCCGTAACGATTT
GTCATTAAAAATCCGCTTCTCGGCTGAAGGCACGCGCAACAGCGCGAGCGGCCTAGGTCTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (49-272)

3D structure

• AlphaFold DB: C6WV12

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.014	95.095	0.523
pilU	Acinetobacter baylyi ADP1	50.975	97.82	0.499
pilU	Vibrio cholerae strain A1552	48	95.368	0.458
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.522	92.916	0.395
pilT	Acinetobacter baylyi ADP1	41.765	92.643	0.387
pilT	Pseudomonas aeruginosa PAK	42.216	91.008	0.384
pilT	Neisseria meningitidis 8013	40.346	94.55	0.381
pilT	Neisseria gonorrhoeae MS11	40.346	94.55	0.381
pilT	Pseudomonas stutzeri DSM 10701	41.617	91.008	0.379
pilT	Acinetobacter nosocomialis M2	40.828	92.098	0.376
pilT	Acinetobacter baumannii D1279779	40.533	92.098	0.373
pilT	Acinetobacter baumannii strain A118	40.533	92.098	0.373
pilT	Legionella pneumophila strain ERS1305867	41.018	91.008	0.373
pilT	Legionella pneumophila strain Lp02	41.018	91.008	0.373
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	38.329	94.55	0.362
pilT	Vibrio cholerae strain A1552	38.329	94.55	0.362