Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EYC54_RS04100 Genome accession   NZ_CP036252
Coordinates   826322..827455 (-) Length   377 a.a.
NCBI ID   WP_131077831.1    Uniprot ID   -
Organism   Xanthomonas oryzae strain NJ611     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 821322..832455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYC54_RS04070 (EYC54_04070) queD 821758..822111 (-) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  EYC54_RS04080 (EYC54_04080) - 822524..823909 (-) 1386 WP_131074543.1 DEAD/DEAH box helicase -
  EYC54_RS04090 (EYC54_04090) - 824218..825276 (+) 1059 WP_207306722.1 fumarylacetoacetate hydrolase family protein -
  EYC54_RS04095 (EYC54_04095) maiA 825320..825982 (+) 663 WP_053503917.1 maleylacetoacetate isomerase -
  EYC54_RS04100 (EYC54_04100) pilU 826322..827455 (-) 1134 WP_131077831.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EYC54_RS04105 (EYC54_04105) - 827554..827937 (+) 384 WP_131077832.1 DUF4398 domain-containing protein -
  EYC54_RS04110 (EYC54_04110) - 827934..828824 (+) 891 WP_053503914.1 hypothetical protein -
  EYC54_RS04115 (EYC54_04115) - 829166..829384 (+) 219 WP_003484103.1 YdcH family protein -
  EYC54_RS04120 (EYC54_04120) - 829625..830995 (+) 1371 WP_053503913.1 pyridoxal-phosphate dependent enzyme -
  EYC54_RS04125 (EYC54_04125) - 831086..832282 (+) 1197 WP_131077833.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41822.26 Da        Isoelectric Point: 6.2391

>NTDB_id=347644 EYC54_RS04100 WP_131077831.1 826322..827455(-) (pilU) [Xanthomonas oryzae strain NJ611]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELHLPQVLKDIILTPRGLVLVVGSTGSGKSTSLAAMIDHRNGTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETLAAPKISHGFL

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=347644 EYC54_RS04100 WP_131077831.1 826322..827455(-) (pilU) [Xanthomonas oryzae strain NJ611]
ATGGATATCGGCTACTTCTTGAAGCTGATGACCGAAAAGAATGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGATTGCCACCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAACTCAACATGGCCATCGCCCTGCCCGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAACAGCGCGGCGAGGTGGGCATGGTGATCCGTGCCATTCGCAGCCGGATCCCCAGCATCGA
AGAGCTCCACCTGCCACAGGTGCTCAAGGACATCATCTTGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTTGCCGCGATGATCGACCACCGCAACGGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGATACCCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAGGCAG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGTATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTCAACCTGCGCGCGGTCATCTCCCAGCG
TCTGGTCAAGGGCCTGGACGGCCGTCGCCGCCCGGCCACCGAAGTGCTGCTCAATACGCCGATGATCCGCGACCTGCTAC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCGCTGCGTGCCGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGTCTCTCCGAAGGCGGCTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGCTGGCTGCGCCGAAGATCAGCC
ACGGGTTTCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.646

95.225

0.501
  pilU Vibrio cholerae strain A1552

49.722

95.491

0.475
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.802

91.247

0.427
  pilT Pseudomonas aeruginosa PAK

43.195

89.655

0.387
  pilT Acinetobacter baumannii strain A118

42.308

89.655

0.379
  pilT Acinetobacter nosocomialis M2

42.308

89.655

0.379
  pilT Acinetobacter baumannii D1279779

42.308

89.655

0.379
  pilT Acinetobacter baylyi ADP1

41.765

90.186

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.012

89.655

0.377
  pilT Neisseria meningitidis 8013

40.058

92.042

0.369
  pilT Neisseria gonorrhoeae MS11

40.058

92.042

0.369
  pilT Vibrio cholerae strain A1552

41.742

88.329

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

88.329

0.369
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369

Multiple sequence alignment