Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EXN74_RS06920 Genome accession   NZ_CP036199
Coordinates   1440385..1441362 (+) Length   325 a.a.
NCBI ID   WP_130588228.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain 16005813     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1435385..1446362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXN74_RS06890 (EXN74_06890) - 1436627..1436989 (-) 363 WP_117386675.1 endonuclease domain-containing protein -
  EXN74_RS06895 (EXN74_06895) hemW 1437053..1438195 (-) 1143 WP_130588226.1 radical SAM family heme chaperone HemW -
  EXN74_RS06900 (EXN74_06900) - 1438188..1438781 (-) 594 WP_059307228.1 XTP/dITP diphosphatase -
  EXN74_RS06905 (EXN74_06905) yggU 1438785..1439081 (-) 297 WP_130588227.1 DUF167 family protein YggU -
  EXN74_RS06910 (EXN74_06910) - 1439078..1439644 (-) 567 WP_032613937.1 YggT family protein -
  EXN74_RS06915 (EXN74_06915) - 1439666..1440367 (-) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  EXN74_RS06920 (EXN74_06920) pilT 1440385..1441362 (+) 978 WP_130588228.1 type IV pilus twitching motility protein PilT Machinery gene
  EXN74_RS06925 (EXN74_06925) - 1441462..1442151 (+) 690 WP_032613957.1 hypothetical protein -
  EXN74_RS06930 (EXN74_06930) ruvX 1442190..1442606 (-) 417 WP_130588229.1 Holliday junction resolvase RuvX -
  EXN74_RS06935 (EXN74_06935) - 1442606..1443166 (-) 561 WP_032613928.1 YqgE/AlgH family protein -
  EXN74_RS06940 (EXN74_06940) gshB 1443258..1444205 (-) 948 WP_059307231.1 glutathione synthase -
  EXN74_RS06945 (EXN74_06945) rsmE 1444225..1444956 (-) 732 WP_130588230.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EXN74_RS06950 (EXN74_06950) endA 1445068..1445775 (-) 708 WP_130588231.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35641.79 Da        Isoelectric Point: 6.7692

>NTDB_id=347480 EXN74_RS06920 WP_130588228.1 1440385..1441362(+) (pilT) [Leclercia adecarboxylata strain 16005813]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVVLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=347480 EXN74_RS06920 WP_130588228.1 1440385..1441362(+) (pilT) [Leclercia adecarboxylata strain 16005813]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGGCCGGATGTGGTGGTGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGGGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTCAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACTGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACTCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAACTGGCCGGCAGTTTGCAGGCGGTGCTGGCGCAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTCATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.492
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.492
  pilT Legionella pneumophila strain Lp02

48.137

99.077

0.477
  pilT Legionella pneumophila strain ERS1305867

48.137

99.077

0.477
  pilT Acinetobacter baylyi ADP1

47.095

100

0.474
  pilT Neisseria meningitidis 8013

46.951

100

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.58

97.538

0.474
  pilT Pseudomonas aeruginosa PAK

47.95

97.538

0.468
  pilT Acinetobacter nosocomialis M2

47.649

98.154

0.468
  pilT Acinetobacter baumannii strain A118

47.649

98.154

0.468
  pilT Acinetobacter baumannii D1279779

47.649

98.154

0.468
  pilT Neisseria gonorrhoeae MS11

47.188

98.462

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

36.728

99.692

0.366

Multiple sequence alignment