Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCANL266_RS08560 Genome accession   NZ_CP035946
Coordinates   1771277..1772344 (-) Length   355 a.a.
NCBI ID   WP_172234038.1    Uniprot ID   -
Organism   Campylobacter canadensis strain LMG 24001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1766277..1777344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCANL266_RS08535 - 1766992..1767537 (-) 546 WP_172234028.1 DUF1882 domain-containing protein -
  CCANL266_RS08540 glyA 1767531..1768757 (-) 1227 WP_172234030.1 serine hydroxymethyltransferase -
  CCANL266_RS08545 lysS 1768770..1770251 (-) 1482 WP_172234032.1 lysine--tRNA ligase -
  CCANL266_RS08550 - 1770264..1770716 (-) 453 WP_172234034.1 Fur family transcriptional regulator -
  CCANL266_RS08555 - 1770720..1771280 (-) 561 WP_172234036.1 CvpA family protein -
  CCANL266_RS08560 pilT 1771277..1772344 (-) 1068 WP_172234038.1 type IV pilus twitching motility protein PilT Machinery gene
  CCANL266_RS08565 gatC 1772360..1772635 (-) 276 WP_172234040.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CCANL266_RS08570 - 1772747..1773256 (+) 510 WP_172234042.1 hypothetical protein -
  CCANL266_RS08575 - 1773256..1774200 (+) 945 WP_172234044.1 hypothetical protein -
  CCANL266_RS08580 - 1774197..1774808 (+) 612 WP_172234046.1 type III pantothenate kinase -
  CCANL266_RS08585 - 1774844..1776193 (+) 1350 WP_172234048.1 FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39946.74 Da        Isoelectric Point: 6.5935

>NTDB_id=345219 CCANL266_RS08560 WP_172234038.1 1771277..1772344(-) (pilT) [Campylobacter canadensis strain LMG 24001]
MENQLLEMNELLAFMKENNASDLHLVPGKRPIIRIHGVLTDLEFDILTPDVIKNSCYSIISDSQKASLEETKELDFAYPV
RGVGRFRGNYYYVEEGKLAAAFRMIPDTIPSLDELKSPAVFKELIKKEKGLILVTGATGSGKSTTLAAMLNEINQTERKH
ILTIEHPVEFMHQSKKSIFSYRNVDIDTKSFKNALKSALRQDPDIILIGEMRDKETIEAAITAAETGHLVFGTLHTNSAA
QTINRIVDSFEGSEQEQIRNMLSFSLNAIVSQCLIPRNGGGRVAIHEILINNFAIANLIRENKNHQIYSQMQLNQQATGM
RTQTQSLVEAVKANYITRELALRYSTNYNELMNLI

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=345219 CCANL266_RS08560 WP_172234038.1 1771277..1772344(-) (pilT) [Campylobacter canadensis strain LMG 24001]
ATGGAAAATCAATTACTAGAAATGAATGAACTTTTAGCGTTTATGAAAGAAAATAACGCTTCAGACTTACACTTAGTTCC
AGGAAAAAGACCAATTATAAGAATACACGGTGTTTTAACGGATTTAGAATTTGATATTTTAACACCAGATGTAATTAAAA
ATTCTTGCTATAGCATTATCTCAGATTCTCAAAAAGCTTCTTTAGAAGAGACAAAAGAATTAGATTTTGCATATCCTGTA
AGAGGGGTTGGTCGTTTTAGGGGAAACTATTATTATGTGGAAGAAGGCAAGCTTGCAGCAGCCTTTCGTATGATTCCTGA
TACCATTCCTAGCCTTGATGAGTTAAAATCTCCTGCAGTTTTTAAAGAATTAATTAAAAAAGAAAAAGGTTTAATTTTAG
TAACAGGAGCAACAGGTAGTGGTAAATCAACAACACTAGCTGCTATGTTAAATGAAATTAATCAAACAGAAAGAAAGCAT
ATTTTAACAATTGAACACCCAGTTGAATTTATGCACCAAAGTAAAAAATCAATTTTTTCATACAGAAACGTTGATATAGA
TACTAAAAGCTTTAAAAATGCGCTTAAATCAGCTTTAAGACAAGACCCTGATATTATCTTAATCGGTGAGATGAGAGATA
AAGAAACAATAGAAGCTGCTATTACTGCTGCTGAAACAGGACACTTAGTTTTTGGAACACTTCATACTAACTCAGCAGCA
CAAACCATTAACCGTATTGTAGATAGCTTTGAAGGAAGCGAACAAGAGCAAATTAGAAATATGCTTTCATTCTCTCTTAA
CGCTATTGTTTCACAATGCTTAATTCCAAGAAATGGCGGTGGCCGTGTTGCGATTCACGAAATTTTAATTAATAACTTTG
CTATTGCAAATTTAATTCGTGAAAATAAAAATCATCAGATTTATTCGCAAATGCAGTTAAATCAACAAGCAACAGGTATG
AGAACACAAACTCAAAGTTTAGTTGAAGCGGTAAAGGCTAATTACATTACTAGAGAATTAGCTTTAAGATATAGTACAAA
TTACAATGAATTGATGAATTTAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

51.603

96.62

0.499
  pilT Neisseria meningitidis 8013

51.603

96.62

0.499
  pilT Legionella pneumophila strain ERS1305867

51.02

96.62

0.493
  pilT Legionella pneumophila strain Lp02

51.02

96.62

0.493
  pilT Pseudomonas aeruginosa PAK

50.599

94.085

0.476
  pilT Acinetobacter baumannii strain A118

50

94.648

0.473
  pilT Acinetobacter nosocomialis M2

50

94.648

0.473
  pilT Acinetobacter baumannii D1279779

50

94.648

0.473
  pilT Vibrio cholerae strain A1552

52.025

90.423

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.025

90.423

0.47
  pilT Pseudomonas stutzeri DSM 10701

49.107

94.648

0.465
  pilT Acinetobacter baylyi ADP1

50.621

90.704

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.714

98.592

0.431
  pilU Vibrio cholerae strain A1552

39.769

97.746

0.389
  pilU Acinetobacter baylyi ADP1

39.368

98.028

0.386
  pilU Pseudomonas stutzeri DSM 10701

39.031

98.873

0.386

Multiple sequence alignment