Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ES815_RS05965 Genome accession   NZ_CP035382
Coordinates   1058728..1059705 (-) Length   325 a.a.
NCBI ID   WP_142487046.1    Uniprot ID   A0AAP9DA64
Organism   Leclercia adecarboxylata strain R25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1053728..1064705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES815_RS05930 (ES815_05975) - 1053736..1054233 (+) 498 WP_142487040.1 SprT family zinc-dependent metalloprotease -
  ES815_RS05935 (ES815_05980) endA 1054328..1055035 (+) 708 WP_142487041.1 deoxyribonuclease I -
  ES815_RS05940 (ES815_05985) rsmE 1055147..1055878 (+) 732 WP_142490023.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ES815_RS05945 (ES815_05990) gshB 1055898..1056845 (+) 948 WP_142487042.1 glutathione synthase -
  ES815_RS05950 (ES815_05995) - 1056937..1057497 (+) 561 WP_142487043.1 YqgE/AlgH family protein -
  ES815_RS05955 (ES815_06000) ruvX 1057497..1057913 (+) 417 WP_142487044.1 Holliday junction resolvase RuvX -
  ES815_RS05960 (ES815_06005) - 1057952..1058641 (-) 690 WP_142487045.1 IclR family transcriptional regulator -
  ES815_RS05965 (ES815_06010) pilT 1058728..1059705 (-) 978 WP_142487046.1 type IV pilus twitching motility protein PilT Machinery gene
  ES815_RS05970 (ES815_06015) - 1059723..1060421 (+) 699 WP_142487047.1 YggS family pyridoxal phosphate-dependent enzyme -
  ES815_RS05975 (ES815_06020) - 1060446..1061012 (+) 567 WP_142487048.1 YggT family protein -
  ES815_RS05980 (ES815_06025) yggU 1061009..1061305 (+) 297 WP_103822821.1 DUF167 family protein YggU -
  ES815_RS05985 (ES815_06030) - 1061309..1061902 (+) 594 WP_142487049.1 XTP/dITP diphosphatase -
  ES815_RS05990 (ES815_06035) hemW 1061895..1063031 (+) 1137 WP_142487050.1 radical SAM family heme chaperone HemW -
  ES815_RS05995 (ES815_06040) - 1063365..1064309 (+) 945 WP_409518831.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35832.03 Da        Isoelectric Point: 6.7668

>NTDB_id=339442 ES815_RS05965 WP_142487046.1 1058728..1059705(-) (pilT) [Leclercia adecarboxylata strain R25]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGEMEFAPFPGPDVVMLLKRWLSDEQQGAWCAQGQVDFAVTLKHHRLRAS
AFAHTQGCSLALRLLPENCPQLCTLDTPRAIPELLTSDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCPSFADGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTWQLPGVIQTGLQAGMQNFEQSRAERR
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=339442 ES815_RS05965 WP_142487046.1 1058728..1059705(-) (pilT) [Leclercia adecarboxylata strain R25]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCGCG
CTGGCGGCGGCGAGGAGAAATGGAATTCGCCCCTTTTCCGGGGCCGGATGTGGTGATGCTGCTCAAACGCTGGCTCAGCG
ACGAACAGCAGGGGGCATGGTGCGCCCAGGGGCAGGTGGACTTTGCCGTTACACTGAAGCACCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGTTGCCCGAAAATTGCCCTCAGCTGTGTACCCTGGACAC
GCCGCGAGCGATCCCCGAGCTGCTCACCAGCGATAACGGGCTGATTCTGGTAACCGGGGCCACCGGCAGCGGTAAATCCA
CCACGCTTGCGGCCATGGTCGACTTTCTCAATCACCACAGCGACGGTCATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAGCGTTGTCTGGTGCAGCAGCGGGAGATTGGCCTGCATTGCCCCTCTTTTGCCGATGGGCTTCGGGC
TGCGCTGCGTGAAGATCCTGACGTGATCCTGTTAGGCGAACTGCGCGACAGCGAAACGATCCGCCTTGCGCTCACCGCCG
CAGAGACCGGACATCTGGTGCTGGCGACCCTGCATACCCGGGGGGCCGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCCGAGGAGAAAGAGCCGGTACGTAAGCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCGAAAGGGA
TAACCAGCAGGGACGCGTCGCGCTGTTCGAGCTCCTGGTTAACACCCCTGCGGCGGCCAATTTGATCCGCGAAGGTAAAA
CCTGGCAACTGCCCGGCGTGATCCAGACCGGGTTGCAGGCAGGGATGCAGAACTTTGAGCAGAGCCGTGCTGAACGCAGG
GCACAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.498
  pilT Vibrio cholerae strain A1552

49.541

100

0.498
  pilT Neisseria meningitidis 8013

47.188

98.462

0.465
  pilT Legionella pneumophila strain Lp02

46.894

99.077

0.465
  pilT Legionella pneumophila strain ERS1305867

46.894

99.077

0.465
  pilT Acinetobacter baylyi ADP1

45.872

100

0.462
  pilT Pseudomonas stutzeri DSM 10701

47.319

97.538

0.462
  pilT Neisseria gonorrhoeae MS11

46.875

98.462

0.462
  pilT Acinetobacter baumannii D1279779

46.708

98.154

0.458
  pilT Acinetobacter nosocomialis M2

46.708

98.154

0.458
  pilT Acinetobacter baumannii strain A118

46.708

98.154

0.458
  pilT Pseudomonas aeruginosa PAK

46.372

97.538

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.312

96.615

0.418
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.382

Multiple sequence alignment