Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EQM13_RS09395 Genome accession   NZ_CP035282
Coordinates   1940316..1941368 (-) Length   350 a.a.
NCBI ID   WP_128752507.1    Uniprot ID   A0A410QD92
Organism   Acidilutibacter cellobiosedens strain JN-28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1935316..1946368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQM13_RS09370 (EQM13_09370) - 1936020..1936685 (-) 666 WP_128752503.1 PilN domain-containing protein -
  EQM13_RS09375 (EQM13_09375) pilM 1936687..1937757 (-) 1071 WP_128752504.1 pilus assembly protein PilM -
  EQM13_RS09380 (EQM13_09380) - 1937769..1938536 (-) 768 WP_128752505.1 A24 family peptidase -
  EQM13_RS18935 (EQM13_09385) - 1938573..1939016 (-) 444 WP_320052438.1 type II secretion system protein -
  EQM13_RS09390 (EQM13_09390) - 1939098..1940303 (-) 1206 WP_128752506.1 type II secretion system F family protein -
  EQM13_RS09395 (EQM13_09395) pilT 1940316..1941368 (-) 1053 WP_128752507.1 type IV pilus twitching motility protein PilT Machinery gene
  EQM13_RS09400 (EQM13_09400) - 1941382..1943055 (-) 1674 WP_071138591.1 GspE/PulE family protein -
  EQM13_RS09405 (EQM13_09405) - 1943520..1944947 (-) 1428 WP_206172646.1 ISLre2 family transposase -
  EQM13_RS09410 (EQM13_09410) - 1945602..1946150 (+) 549 WP_161567209.1 YhcN/YlaJ family sporulation lipoprotein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38791.06 Da        Isoelectric Point: 7.2026

>NTDB_id=338893 EQM13_RS09395 WP_128752507.1 1940316..1941368(-) (pilT) [Acidilutibacter cellobiosedens strain JN-28]
MEISDLIKVGIKLKASDIHITVGVPPVFRINGILQNYGENIFTPKDTEYLVKEILSEDKFKILEEKGELDISFSSSGVGR
FRINVFKQRGSFGMALRIIPLTIPTMDELGIPPIVKDLSMLPRGLILVTGPTGSGKSTTLASIVDLINHNKKYHILTLED
PIEYLHKHDKSIVNQREIGSDSLSFANALRAALRQDPDVILVGEMRDLETMSIALTAAETGHLVLSTLHTIGAASTIDRI
IDVFPSYQQQQVRIQLSSVIQGIISQQLLPTIDGKGRIAAFEIMVATPAIRNLIREEKTHQIDTAIQTGSKYGMQTMDNS
MLKLFNKGKISEETVLSQSVFPDVIKRYLK

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=338893 EQM13_RS09395 WP_128752507.1 1940316..1941368(-) (pilT) [Acidilutibacter cellobiosedens strain JN-28]
ATGGAGATTTCGGATTTAATAAAAGTGGGGATAAAATTAAAAGCATCGGATATTCATATAACCGTTGGAGTTCCTCCTGT
ATTTAGGATTAACGGTATTCTTCAAAATTATGGGGAAAATATTTTTACTCCGAAGGATACGGAATATTTGGTCAAAGAAA
TTTTATCCGAAGATAAATTTAAAATTCTTGAGGAAAAAGGAGAACTTGATATATCCTTTTCAAGTTCAGGCGTAGGAAGG
TTTAGGATTAACGTTTTTAAACAGAGAGGAAGCTTTGGAATGGCTCTAAGAATAATTCCTTTGACAATACCCACTATGGA
TGAATTGGGGATTCCTCCAATTGTAAAGGATTTATCCATGCTGCCCAGAGGCCTTATATTAGTTACGGGACCTACAGGAA
GTGGTAAATCTACAACTCTTGCTTCCATAGTGGATTTGATAAACCATAACAAAAAATACCATATACTGACTTTAGAGGAT
CCCATAGAGTATCTTCATAAGCATGACAAAAGCATAGTCAATCAAAGAGAAATAGGAAGTGACTCCTTATCCTTTGCCAA
TGCATTGAGAGCTGCTTTAAGGCAGGACCCCGATGTAATACTTGTAGGTGAGATGAGAGATCTTGAAACCATGAGTATTG
CGCTGACGGCCGCAGAGACAGGGCATTTGGTTCTTTCTACTCTTCATACTATAGGAGCAGCCAGTACCATAGACAGAATA
ATAGATGTATTTCCTTCTTATCAGCAGCAGCAGGTGAGAATTCAACTTTCTTCTGTAATACAGGGAATAATATCTCAACA
GCTTCTTCCGACGATTGATGGAAAAGGGAGAATTGCAGCCTTTGAGATAATGGTTGCCACTCCCGCTATCAGAAATTTAA
TAAGAGAAGAAAAGACTCACCAAATAGATACGGCAATACAGACAGGAAGCAAATATGGGATGCAAACCATGGATAATTCC
ATGTTGAAACTTTTCAATAAAGGAAAGATATCCGAGGAAACTGTATTAAGCCAATCTGTATTTCCCGATGTTATTAAAAG
GTATTTGAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A410QD92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.585

99.143

0.511
  pilT Pseudomonas aeruginosa PAK

51.02

98

0.5
  pilT Acinetobacter baumannii D1279779

50.437

98

0.494
  pilT Acinetobacter baumannii strain A118

50.437

98

0.494
  pilT Acinetobacter nosocomialis M2

50.437

98

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.888

96.571

0.491
  pilT Vibrio cholerae strain A1552

50.888

96.571

0.491
  pilT Legionella pneumophila strain Lp02

49.565

98.571

0.489
  pilT Legionella pneumophila strain ERS1305867

49.565

98.571

0.489
  pilT Pseudomonas stutzeri DSM 10701

49.563

98

0.486
  pilT Acinetobacter baylyi ADP1

49.271

98

0.483
  pilT Neisseria meningitidis 8013

49.85

95.143

0.474
  pilT Neisseria gonorrhoeae MS11

49.254

95.714

0.471
  pilU Pseudomonas stutzeri DSM 10701

41.964

96

0.403
  pilU Acinetobacter baylyi ADP1

39.601

100

0.397
  pilU Vibrio cholerae strain A1552

38.857

100

0.389

Multiple sequence alignment