Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LHK_RS00150 Genome accession   NC_012559
Coordinates   28126..29169 (-) Length   347 a.a.
NCBI ID   WP_012695523.1    Uniprot ID   C1D9C9
Organism   Laribacter hongkongensis HLHK9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 23126..34169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LHK_RS00125 (LHK_00027) - 23696..24592 (+) 897 WP_012695518.1 transporter substrate-binding domain-containing protein -
  LHK_RS00130 (LHK_00028) - 24687..25433 (+) 747 WP_012695519.1 amino acid ABC transporter permease -
  LHK_RS00135 (LHK_00029) - 25445..26092 (+) 648 WP_227460212.1 amino acid ABC transporter permease -
  LHK_RS00140 (LHK_00030) - 26104..26832 (+) 729 WP_012695521.1 amino acid ABC transporter ATP-binding protein -
  LHK_RS00145 thpR 27344..27892 (-) 549 WP_052292520.1 RNA 2',3'-cyclic phosphodiesterase -
  LHK_RS00150 (LHK_00032) pilT 28126..29169 (-) 1044 WP_012695523.1 type IV pilus twitching motility protein PilT Machinery gene
  LHK_RS00155 (LHK_00033) - 29311..30012 (+) 702 WP_012695524.1 YggS family pyridoxal phosphate-dependent enzyme -
  LHK_RS00160 (LHK_00034) proC 30040..30846 (+) 807 WP_027823947.1 pyrroline-5-carboxylate reductase -
  LHK_RS00165 (LHK_00035) - 30852..31433 (+) 582 WP_012695526.1 YggT family protein -
  LHK_RS00170 (LHK_00036) - 31582..31887 (+) 306 WP_012695527.1 c-type cytochrome -
  LHK_RS00175 (LHK_00037) dksA 32143..32559 (+) 417 WP_012695528.1 RNA polymerase-binding protein DksA -
  LHK_RS00180 (LHK_00038) aroG 32704..33774 (+) 1071 WP_012695529.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37921.55 Da        Isoelectric Point: 6.4320

>NTDB_id=33741 LHK_RS00150 WP_012695523.1 28126..29169(-) (pilT) [Laribacter hongkongensis HLHK9]
MDIAELLTFTVHQQASDLHLSAGLPPMIRVHGDMRRINLPPLSHDDVQDMVYPILSAPQRSHYEQQLELDLSFELPGLAR
FRVNLFVQSRGAGAVFRTIPDRIPTLASLGAPAILDELVASPRGLVLVTGPTGSGKSTTLAAMLDAVNERQAAHILTIED
PIEFVHTSRRSLINQREVGPHTRSFAHALRSALREDPDVILIGELRDLDTIRLALTAAETGHLVFGTLHTSSATKTVDRI
VDVFPGDEKDMVRAMLSESLRAVVSQTLLKRRDGQGRLAAYEIMLGTPAIRNLIRENKIAQMYSVMQTSQQAGMQTLDQA
LQALVGAGLVSADEARMRAVDKDALVA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=33741 LHK_RS00150 WP_012695523.1 28126..29169(-) (pilT) [Laribacter hongkongensis HLHK9]
ATGGATATTGCCGAACTGCTCACCTTTACCGTCCACCAGCAGGCATCCGACCTGCACCTGTCGGCCGGGCTGCCGCCGAT
GATCCGCGTGCATGGCGACATGCGCCGCATCAACCTGCCCCCGCTGTCGCACGATGACGTGCAGGACATGGTGTACCCCA
TCCTGAGCGCGCCGCAGCGCAGCCATTACGAGCAGCAGCTGGAGCTGGACCTATCGTTCGAGCTGCCCGGCCTGGCGCGC
TTCCGGGTCAACCTGTTCGTGCAGAGCCGGGGCGCCGGCGCGGTATTCCGTACCATTCCCGACCGGATTCCCACGCTGGC
ATCGCTGGGCGCGCCGGCCATCCTCGACGAACTGGTCGCCAGCCCCCGCGGGCTGGTGCTGGTGACCGGTCCGACCGGCT
CGGGCAAAAGCACCACGCTGGCGGCCATGCTCGACGCCGTCAACGAGCGGCAGGCCGCCCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACACCAGCCGCCGCAGCCTCATCAACCAGCGCGAAGTCGGCCCGCATACCCGCAGTTTTGCCCA
CGCCCTGCGCAGCGCCCTGCGCGAAGACCCGGACGTGATCCTGATCGGCGAACTGCGCGACCTCGACACCATCCGGCTGG
CGCTGACCGCTGCCGAAACCGGCCACCTGGTATTCGGCACCCTGCATACCAGCTCGGCCACCAAAACCGTCGACCGCATC
GTCGACGTGTTCCCCGGCGACGAAAAGGACATGGTGCGCGCCATGCTGTCGGAAAGCCTGCGCGCCGTGGTGTCGCAAAC
CCTGCTCAAGCGCCGCGACGGACAGGGCCGGCTGGCCGCCTATGAAATCATGCTGGGTACGCCGGCCATCCGTAACCTGA
TCCGCGAAAACAAGATTGCCCAGATGTACTCGGTCATGCAGACCAGCCAGCAGGCCGGCATGCAGACGCTCGACCAGGCA
TTGCAGGCGCTGGTGGGCGCTGGCCTGGTCAGCGCCGACGAGGCCCGGATGCGCGCGGTCGACAAGGACGCACTGGTTGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1D9C9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

67.552

97.695

0.66
  pilT Acinetobacter nosocomialis M2

67.552

97.695

0.66
  pilT Acinetobacter baumannii strain A118

67.552

97.695

0.66
  pilT Pseudomonas aeruginosa PAK

66.962

97.695

0.654
  pilT Pseudomonas stutzeri DSM 10701

66.372

97.695

0.648
  pilT Neisseria meningitidis 8013

65.217

99.424

0.648
  pilT Neisseria gonorrhoeae MS11

64.928

99.424

0.646
  pilT Legionella pneumophila strain ERS1305867

65.497

98.559

0.646
  pilT Legionella pneumophila strain Lp02

65.497

98.559

0.646
  pilT Acinetobacter baylyi ADP1

66.369

96.83

0.643
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.497

97.406

0.628
  pilT Vibrio cholerae strain A1552

64.497

97.406

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.613

98.271

0.507
  pilU Pseudomonas stutzeri DSM 10701

40.708

97.695

0.398
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Acinetobacter baylyi ADP1

40.179

96.83

0.389

Multiple sequence alignment