Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FZN32_RS20255 Genome accession   NZ_CP043406
Coordinates   4161458..4162438 (+) Length   326 a.a.
NCBI ID   WP_001055632.1    Uniprot ID   -
Organism   Escherichia coli strain NMBU-W13E19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4156458..4167438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZN32_RS20225 (FZN32_20755) - 4156836..4157822 (-) 987 WP_001530835.1 TRAP transporter substrate-binding protein -
  FZN32_RS20230 (FZN32_20760) hemW 4158135..4159271 (-) 1137 WP_000239986.1 radical SAM family heme chaperone HemW -
  FZN32_RS20235 (FZN32_20765) rdgB 4159264..4159857 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  FZN32_RS20240 (FZN32_20770) yggU 4159865..4160155 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  FZN32_RS20245 (FZN32_20775) yggT 4160152..4160718 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  FZN32_RS20250 (FZN32_20780) yggS 4160736..4161440 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  FZN32_RS20255 (FZN32_20785) pilT 4161458..4162438 (+) 981 WP_001055632.1 type IV pilus twitching motility protein PilT Machinery gene
  FZN32_RS20260 (FZN32_20790) ruvX 4162651..4163067 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  FZN32_RS20265 (FZN32_20795) yqgE 4163067..4163630 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  FZN32_RS20270 (FZN32_20800) gshB 4163739..4164689 (-) 951 WP_000593273.1 glutathione synthase -
  FZN32_RS20275 (FZN32_20805) rsmE 4164702..4165433 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FZN32_RS20280 (FZN32_20810) endA 4165513..4166220 (-) 708 WP_000286500.1 deoxyribonuclease I -
  FZN32_RS20285 (FZN32_20815) yggI 4166315..4166812 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36000.25 Da        Isoelectric Point: 5.7980

>NTDB_id=336965 FZN32_RS20255 WP_001055632.1 4161458..4162438(+) (pilT) [Escherichia coli strain NMBU-W13E19]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAVPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPLILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=336965 FZN32_RS20255 WP_001055632.1 4161458..4162438(+) (pilT) [Escherichia coli strain NMBU-W13E19]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGTGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACTGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365

Multiple sequence alignment