Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EO763_RS18125 Genome accession   NZ_CP034938
Coordinates   4016072..4017088 (-) Length   338 a.a.
NCBI ID   WP_161529788.1    Uniprot ID   -
Organism   Pectobacterium odoriferum strain JK2.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4011072..4022088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO763_RS18110 (EO763_18395) nsrR 4011452..4011877 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  EO763_RS18115 (EO763_18400) - 4012172..4013743 (+) 1572 WP_161529786.1 methyl-accepting chemotaxis protein -
  EO763_RS18120 (EO763_18405) - 4014271..4016103 (+) 1833 WP_161529787.1 diguanylate cyclase -
  EO763_RS18125 (EO763_18410) pilT 4016072..4017088 (-) 1017 WP_161529788.1 type IV pilus twitching motility protein PilT Machinery gene
  EO763_RS18130 (EO763_18415) - 4017110..4017823 (+) 714 WP_161529789.1 YggS family pyridoxal phosphate-dependent enzyme -
  EO763_RS18135 (EO763_18420) proC 4017925..4018746 (+) 822 WP_161530438.1 pyrroline-5-carboxylate reductase -
  EO763_RS18140 (EO763_18425) - 4018796..4019350 (+) 555 WP_010279107.1 YggT family protein -
  EO763_RS18145 (EO763_18430) yggU 4019347..4019637 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  EO763_RS18150 (EO763_18435) - 4019669..4020262 (+) 594 WP_039470191.1 XTP/dITP diphosphatase -
  EO763_RS18155 (EO763_18440) hemW 4020255..4021394 (+) 1140 WP_161529790.1 radical SAM family heme chaperone HemW -
  EO763_RS18160 (EO763_18445) - 4021626..4021886 (+) 261 WP_072014018.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36752.08 Da        Isoelectric Point: 6.5660

>NTDB_id=333535 EO763_RS18125 WP_161529788.1 4016072..4017088(-) (pilT) [Pectobacterium odoriferum strain JK2.1]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQREQLRQIGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSAQPSLEALHAPPVFSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDCCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFALALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LVDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCQDGLISHSYALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=333535 EO763_RS18125 WP_161529788.1 4016072..4017088(-) (pilT) [Pectobacterium odoriferum strain JK2.1]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCATCCGCCAGT
GTTGCGTGTGGACGGGAGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGATCAGGTGGCGCAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGATTGGTCAGGTGGACGGCGCATTAATGCTGCCTGATGGGCAA
CGCCTGCGGGTAAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGTTGCGCGTTATTCCATCCGCTCAGCCTTCATT
GGAAGCGCTACACGCGCCACCCGTTTTCTCGACACTGCTGGAGAAACCGAACGGACTGATCCTGATTACCGGTGCGACAG
GGAGCGGTAAATCCACAACGCTGGCGGCCATGCTTGGGGCGTTAAACGACTGCTGCGATCGCCATGTGATTACGCTGGAG
GACCCGATCGAGTTTATCCATGCCAGCCGACGCTGCCTGATTCAACAGCGAGAGATCGGTGCGCACAGCGCGTCGTTTGC
ACTGGCATTACGGGCCGCACTGCGGGAGGATCCCGATGTCATTCTGTTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACTGGCCATCTGGTGTTGTCGACGCTGCATACCCGCAGCGCATCGCAGGCGGTCGATCGT
CTGGTTGACGTCTTTCCTGGTGAAGAAAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCGGTGGTGACACA
AAAGTTGTTGCCTGCCGCGCAAGGGGGGCGAATCGCGCTTTATGAAGTGCTGACCGCCACCGCGGCAGTCAGTAATCTGA
TTCGGGAAGGGAAAACGCACCAACTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAGACGTTTGAGCAAAGC
TACCAGCAGCGCTGTCAGGATGGGCTTATTTCACACAGTTACGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.904

98.225

0.5
  pilT Neisseria gonorrhoeae MS11

50.602

98.225

0.497
  pilT Pseudomonas stutzeri DSM 10701

49.851

99.112

0.494
  pilT Pseudomonas aeruginosa PAK

49.552

99.112

0.491
  pilT Acinetobacter baylyi ADP1

49.112

100

0.491
  pilT Legionella pneumophila strain ERS1305867

48.657

99.112

0.482
  pilT Legionella pneumophila strain Lp02

48.657

99.112

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.466

99.704

0.393
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

37.278

100

0.373

Multiple sequence alignment