Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ENC22_RS27850 Genome accession   NZ_CP034836
Coordinates   6269980..6271014 (-) Length   344 a.a.
NCBI ID   WP_011400052.1    Uniprot ID   A0AAE5XN00
Organism   Hahella sp. KA22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 6253025..6287463 6269980..6271014 within 0


Gene organization within MGE regions


Location: 6253025..6287463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENC22_RS27765 (ENC22_27765) - 6253025..6254281 (-) 1257 WP_127973814.1 site-specific integrase -
  ENC22_RS27775 (ENC22_27775) - 6254633..6255499 (-) 867 WP_127973816.1 YicC/YloC family endoribonuclease -
  ENC22_RS27780 (ENC22_27780) - 6255759..6256139 (-) 381 WP_011400037.1 hypothetical protein -
  ENC22_RS27785 (ENC22_27785) rph 6256421..6257137 (+) 717 WP_127973817.1 ribonuclease PH -
  ENC22_RS27790 (ENC22_27790) - 6257226..6257999 (-) 774 WP_127973818.1 exodeoxyribonuclease III -
  ENC22_RS27795 (ENC22_27795) pyrE 6258081..6258728 (+) 648 WP_011400040.1 orotate phosphoribosyltransferase -
  ENC22_RS27800 (ENC22_27800) - 6258725..6259504 (-) 780 WP_241566900.1 sulfite exporter TauE/SafE family protein -
  ENC22_RS27805 (ENC22_27805) - 6259588..6260232 (+) 645 WP_127973820.1 ABC transporter ATPase -
  ENC22_RS27810 (ENC22_27810) - 6260300..6260674 (-) 375 WP_127973821.1 hypothetical protein -
  ENC22_RS27815 (ENC22_27815) - 6261042..6262328 (+) 1287 WP_127973822.1 MgtC/SapB family protein -
  ENC22_RS27820 (ENC22_27820) yegQ 6262360..6263700 (+) 1341 WP_127973823.1 tRNA 5-hydroxyuridine modification protein YegQ -
  ENC22_RS27825 (ENC22_27825) - 6263932..6264417 (+) 486 WP_127973824.1 fibronectin type III domain-containing protein -
  ENC22_RS27830 (ENC22_27830) - 6264459..6265424 (-) 966 WP_127973825.1 homoserine kinase -
  ENC22_RS27835 (ENC22_27835) - 6265509..6265787 (-) 279 WP_127973826.1 DUF2782 domain-containing protein -
  ENC22_RS27840 (ENC22_27840) polA 6265974..6268709 (+) 2736 WP_127973827.1 DNA polymerase I -
  ENC22_RS27845 (ENC22_27845) pilU 6268790..6269917 (-) 1128 WP_127973828.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ENC22_RS27850 (ENC22_27850) pilT 6269980..6271014 (-) 1035 WP_011400052.1 type IV pilus twitching motility protein PilT Machinery gene
  ENC22_RS27855 (ENC22_27855) - 6271172..6271858 (+) 687 WP_127973829.1 YggS family pyridoxal phosphate-dependent enzyme -
  ENC22_RS27860 (ENC22_27860) proC 6271961..6272791 (+) 831 WP_127973830.1 pyrroline-5-carboxylate reductase -
  ENC22_RS27865 (ENC22_27865) - 6272866..6273405 (+) 540 WP_127973831.1 YggT family protein -
  ENC22_RS27870 (ENC22_27870) - 6273398..6273706 (+) 309 WP_127973832.1 DUF167 family protein -
  ENC22_RS27875 (ENC22_27875) - 6273892..6275850 (+) 1959 WP_127973833.1 dynamin family protein -
  ENC22_RS27880 (ENC22_27880) - 6276024..6277181 (+) 1158 WP_127973834.1 homoserine O-acetyltransferase -
  ENC22_RS27885 (ENC22_27885) metW 6277171..6277764 (+) 594 WP_127973835.1 methionine biosynthesis protein MetW -
  ENC22_RS27890 (ENC22_27890) - 6277772..6278239 (+) 468 WP_241566901.1 DUF4426 domain-containing protein -
  ENC22_RS27895 (ENC22_27895) - 6278284..6278883 (+) 600 WP_127973836.1 XTP/dITP diphosphatase -
  ENC22_RS27900 (ENC22_27900) hemW 6278876..6280042 (+) 1167 WP_127973837.1 radical SAM family heme chaperone HemW -
  ENC22_RS27905 (ENC22_27905) - 6280102..6280629 (+) 528 WP_127973838.1 hypothetical protein -
  ENC22_RS27910 (ENC22_27910) trmB 6280664..6281350 (-) 687 WP_127973839.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ENC22_RS27915 (ENC22_27915) - 6281884..6282735 (+) 852 WP_127973840.1 hypothetical protein -
  ENC22_RS27920 (ENC22_27920) - 6283044..6284576 (-) 1533 WP_127973841.1 YifB family Mg chelatase-like AAA ATPase -
  ENC22_RS27925 (ENC22_27925) - 6284683..6284961 (-) 279 WP_127973842.1 accessory factor UbiK family protein -
  ENC22_RS27930 (ENC22_27930) glnK 6285373..6285711 (+) 339 WP_011400096.1 P-II family nitrogen regulator -
  ENC22_RS27935 (ENC22_27935) - 6285763..6287034 (+) 1272 WP_127973843.1 ammonium transporter -
  ENC22_RS27940 (ENC22_27940) - 6287212..6287463 (+) 252 WP_127973844.1 SemiSWEET transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38015.82 Da        Isoelectric Point: 6.3477

>NTDB_id=333355 ENC22_RS27850 WP_011400052.1 6269980..6271014(-) (pilT) [Hahella sp. KA22]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGQVFKDIALKPRGLCLVTGPTGSGKSTTLAAMIDFINDTRYDHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGAALGMQTLDQCL
HNLISKGLISREAAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=333355 ENC22_RS27850 WP_011400052.1 6269980..6271014(-) (pilT) [Hahella sp. KA22]
ATGGACATTACCGAGTTGCTTGCGTTCTCTGCTAAGCAGGGCGCTTCCGACTTACACCTATCTGCCGGGTTGCCTCCCAT
GATACGCGTCGACGGGGATGTCAGGCGAATTAACCTGCCCGCGATGGAGCATAAAGAAGTACACGCGCTGATTTACGATA
TCATGAACGATAAACAGCGTAAGGATTTCGAAGAATTTCTGGAAACGGATTTCTCGTTCGAAGTTCCCGGGGTCGCCCGT
TTCCGGGTTAACGCCTTCAACCAGAACCGCGGCGCAGGGGCTGTATTCCGGACCATCCCCTCCAAAGTGCTGACGATGGA
AGACTTGGGTATGGGGCAGGTGTTCAAGGATATCGCTCTGAAGCCTCGCGGACTCTGTCTGGTCACAGGACCGACCGGTT
CCGGTAAGAGTACGACGCTGGCGGCGATGATCGACTTTATCAACGACACCCGTTACGACCACATTCTCACTATCGAAGAC
CCTATCGAGTTCGTGCACGAAAGTAAGAAGTGTCTGGTGAACCAGCGCGAAGTGCACCGTGATACGTTAGGGTTCAGTGA
AGCGCTGCGTTCAGCCCTGCGGGAAGACCCGGATATTATCCTGGTTGGTGAGATGCGGGACTTGGAAACCATCCGTCTGG
CGCTGACTGCTGCGGAAACCGGACACTTGGTATTCGGCACCCTGCACACCACCTCCGCCGCGAAAACCATTGACCGGGTG
GTCGACGTGTTTCCCGCGGAAGAAAAAGCCATGGTAAGATCAATGCTCTCCGAATCGTTGCAGGCGGTCGTCTCGCAGAC
CCTTCTGAAGAAGGTCGGCGGCGGTCGTGTGGCGGCCCATGAAATCATGATCGGTACGCCGGCGATTCGTAACCTGATAC
GCGAAGACAAAGTCGCCCAGATGTACTCGTCTATCCAGACCGGCGCCGCGTTGGGTATGCAGACCCTTGACCAGTGTCTG
CACAATTTGATTTCTAAAGGGCTGATCAGCCGCGAAGCCGCGCGCGAAAAAGCCAAAATGCCGGAGAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

91.57

100

0.916
  pilT Pseudomonas aeruginosa PAK

90.988

100

0.91
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.837

0.672
  pilT Vibrio cholerae strain A1552

67.941

98.837

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.384

0.416
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment