Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ELK40_RS19030 Genome accession   NZ_CP034769
Coordinates   3829417..3830397 (-) Length   326 a.a.
NCBI ID   WP_127732269.1    Uniprot ID   -
Organism   Enterobacter sp. N18-03635     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3824417..3835397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELK40_RS19000 (ELK40_19000) - 3825294..3825791 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  ELK40_RS19005 (ELK40_19005) endA 3825886..3826593 (+) 708 WP_115876242.1 deoxyribonuclease I -
  ELK40_RS19010 (ELK40_19010) rsmE 3826645..3827376 (+) 732 WP_127732265.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ELK40_RS19015 (ELK40_19015) gshB 3827396..3828343 (+) 948 WP_047173570.1 glutathione synthase -
  ELK40_RS19020 (ELK40_19020) - 3828431..3828991 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  ELK40_RS19025 (ELK40_19025) ruvX 3828991..3829407 (+) 417 WP_127732267.1 Holliday junction resolvase RuvX -
  ELK40_RS19030 (ELK40_19030) pilT 3829417..3830397 (-) 981 WP_127732269.1 type IV pilus twitching motility protein PilT Machinery gene
  ELK40_RS19035 (ELK40_19035) - 3830415..3831116 (+) 702 WP_115876245.1 YggS family pyridoxal phosphate-dependent enzyme -
  ELK40_RS19040 (ELK40_19040) - 3831138..3831704 (+) 567 WP_111963226.1 YggT family protein -
  ELK40_RS19045 (ELK40_19045) yggU 3831701..3831997 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  ELK40_RS19050 (ELK40_19050) - 3832001..3832594 (+) 594 WP_127732271.1 XTP/dITP diphosphatase -
  ELK40_RS19055 (ELK40_19055) hemW 3832587..3833729 (+) 1143 WP_127732273.1 radical SAM family heme chaperone HemW -
  ELK40_RS19060 (ELK40_19060) - 3833938..3834654 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  ELK40_RS19065 (ELK40_19065) - 3834711..3835037 (-) 327 WP_127732275.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35510.46 Da        Isoelectric Point: 6.4990

>NTDB_id=332950 ELK40_RS19030 WP_127732269.1 3829417..3830397(-) (pilT) [Enterobacter sp. N18-03635]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGTWWANGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLATLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETVRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGLQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=332950 ELK40_RS19030 WP_127732269.1 3829417..3830397(-) (pilT) [Enterobacter sp. N18-03635]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCTGACGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAATGGACAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGTCTGCGCGGG
AGTGCCTTTAAGCAGATGCGCGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTACGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CTACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATTGGCCAGCACAGTCCGTCATTTGCCGACGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGGTCCGCCTGGCGCTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTCCGGGCAGTGCTGGCGCAGAAGCTGCTTCC
CGATCTGCAGGACGGCCGCGTCGCGCTGTATGAACTGCTGGTGAACACCGCTGCGGCGGCAAATCTTATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAAACCGGTCTGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment