Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EKH84_RS11060 Genome accession   NZ_CP034675
Coordinates   2497503..2498567 (+) Length   354 a.a.
NCBI ID   WP_127066341.1    Uniprot ID   A0A5B9YDH6
Organism   Cellulosilyticum sp. WCF-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2492503..2503567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKH84_RS11050 (EKH84_11055) - 2493007..2495625 (+) 2619 WP_127066339.1 YfhO family protein -
  EKH84_RS11055 (EKH84_11060) - 2495806..2497488 (+) 1683 WP_127066340.1 GspE/PulE family protein -
  EKH84_RS11060 (EKH84_11065) pilT 2497503..2498567 (+) 1065 WP_127066341.1 type IV pilus twitching motility protein PilT Machinery gene
  EKH84_RS11065 (EKH84_11070) - 2498693..2499898 (+) 1206 WP_127066342.1 type II secretion system F family protein -
  EKH84_RS11070 (EKH84_11075) - 2499953..2500372 (+) 420 WP_127066343.1 type II secretion system protein -
  EKH84_RS11075 (EKH84_11080) - 2500447..2501223 (+) 777 WP_127066344.1 A24 family peptidase -
  EKH84_RS11080 (EKH84_11085) - 2501241..2501729 (+) 489 WP_190283303.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EKH84_RS11085 (EKH84_11090) - 2501742..2502353 (+) 612 WP_127066346.1 type II secretion system protein -
  EKH84_RS11090 (EKH84_11095) pilM 2502387..2503391 (+) 1005 WP_127066347.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39547.56 Da        Isoelectric Point: 6.8393

>NTDB_id=332413 EKH84_RS11060 WP_127066341.1 2497503..2498567(+) (pilT) [Cellulosilyticum sp. WCF-2]
MDINDLLEIVVQEKASDLHLTVGIPPTLRLHGQLIHLDLPPLKEADTRELISQLLEGKPIITERFREKGEADFSYALQDI
GRFRVNVYKQRGSWATALRLVGVEIPTMEELGLPRKILESLADNKRGLVLVTGPTGSGKSTTLATMIDYMNKKRQEHILT
LEDPIEYLHKHKNSMVNQREIGFDSNSYANGLRAALREDPDVILVGEMRDQETIATAITAAETGHLVLSTLHTIGAPQTI
DRIIDVFPPHQQQQIRVQIANILKGVISQQLLPRSDIKGRCVALEIMISNAAIANLVREEKTHQIYSQIQTGTLAGMITM
DASLANLYKKGAISLETAYQYAQNRDELKRILGR

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=332413 EKH84_RS11060 WP_127066341.1 2497503..2498567(+) (pilT) [Cellulosilyticum sp. WCF-2]
ATGGACATTAATGATTTGTTGGAAATTGTCGTACAGGAAAAAGCATCAGATTTACATCTAACAGTAGGAATTCCACCTAC
ATTAAGATTACATGGCCAATTAATCCACTTGGATTTACCACCATTAAAAGAAGCCGATACAAGAGAACTCATTAGTCAGC
TTTTAGAAGGAAAGCCTATTATTACAGAAAGATTTAGAGAAAAAGGTGAAGCGGACTTTTCCTATGCTTTACAAGATATA
GGGCGTTTTCGTGTAAATGTTTATAAACAAAGAGGAAGTTGGGCAACAGCATTACGATTGGTAGGCGTTGAAATACCTAC
TATGGAGGAATTAGGACTGCCTAGAAAGATACTGGAAAGCTTAGCAGATAATAAGAGAGGCTTAGTATTAGTCACAGGTC
CTACAGGTAGTGGCAAATCAACTACATTGGCTACTATGATAGATTATATGAATAAAAAAAGGCAAGAGCATATCTTAACA
TTAGAAGATCCGATAGAGTATTTACATAAACATAAAAATAGTATGGTTAATCAAAGGGAGATAGGTTTTGACAGCAATAG
CTATGCCAATGGCTTACGAGCTGCTTTGCGTGAAGACCCAGATGTTATTTTGGTAGGAGAAATGCGAGATCAAGAAACCA
TTGCTACAGCCATTACTGCAGCAGAGACAGGTCATTTAGTATTAAGTACATTACATACCATAGGAGCACCTCAAACGATT
GATAGAATTATAGATGTATTTCCACCCCATCAGCAGCAGCAAATTAGGGTTCAGATCGCTAATATCTTAAAAGGGGTTAT
CTCGCAGCAGTTATTGCCAAGAAGTGATATTAAGGGAAGATGTGTAGCATTAGAAATCATGATTAGTAACGCCGCCATAG
CTAATTTGGTTAGAGAGGAAAAGACTCATCAAATTTATTCCCAGATTCAAACAGGAACCTTAGCTGGTATGATTACCATG
GATGCTTCTCTAGCAAATCTTTATAAAAAAGGAGCAATCTCTTTAGAAACAGCCTATCAATATGCGCAAAATAGAGATGA
ACTTAAGCGAATTCTAGGAAGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B9YDH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.268

100

0.514
  pilT Acinetobacter baylyi ADP1

50.872

97.175

0.494
  pilT Legionella pneumophila strain ERS1305867

49.855

97.458

0.486
  pilT Legionella pneumophila strain Lp02

49.855

97.458

0.486
  pilT Acinetobacter nosocomialis M2

49.271

96.893

0.477
  pilT Acinetobacter baumannii D1279779

49.271

96.893

0.477
  pilT Acinetobacter baumannii strain A118

49.271

96.893

0.477
  pilT Pseudomonas aeruginosa PAK

48.688

96.893

0.472
  pilT Neisseria meningitidis 8013

47.564

98.588

0.469
  pilT Neisseria gonorrhoeae MS11

47.278

98.588

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.813

96.893

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.949

94.068

0.46
  pilT Vibrio cholerae strain A1552

48.949

94.068

0.46
  pilU Vibrio cholerae strain A1552

40.909

99.435

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.688

98.588

0.401
  pilU Acinetobacter baylyi ADP1

38.068

99.435

0.379

Multiple sequence alignment