Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EJ065_RS30155 Genome accession   NZ_CP034669
Coordinates   7523573..7524685 (-) Length   370 a.a.
NCBI ID   WP_128798854.1    Uniprot ID   -
Organism   Corallococcus coralloides strain B035     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7518573..7529685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ065_RS30140 (EJ065_5990) - 7519237..7520670 (-) 1434 WP_128798852.1 sigma-54 dependent transcriptional regulator -
  EJ065_RS30145 (EJ065_5991) - 7520667..7522235 (-) 1569 WP_205694658.1 ATP-binding protein -
  EJ065_RS30150 (EJ065_5992) - 7522264..7523517 (-) 1254 WP_128798853.1 type II secretion system F family protein -
  EJ065_RS30155 (EJ065_5993) pilT 7523573..7524685 (-) 1113 WP_128798854.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EJ065_RS30160 (EJ065_5994) pilB 7524734..7526434 (-) 1701 WP_128798855.1 type IV-A pilus assembly ATPase PilB -
  EJ065_RS30165 (EJ065_5995) - 7526654..7527580 (-) 927 WP_128798856.1 bifunctional riboflavin kinase/FAD synthetase -
  EJ065_RS30170 (EJ065_5996) - 7527662..7528282 (+) 621 WP_120620274.1 tRNA (guanine-N7)-methyltransferase -
  EJ065_RS30175 (EJ065_5997) - 7528458..7529501 (+) 1044 WP_128798857.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40477.68 Da        Isoelectric Point: 8.2875

>NTDB_id=332317 EJ065_RS30155 WP_128798854.1 7523573..7524685(-) (pilT) [Corallococcus coralloides strain B035]
MANLHQLLKAMVEKGSSDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDIVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQALVAKQGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLARRLISQDEAFGRSSDPEELRNILATGGGPGGAQRPAGGAGAR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=332317 EJ065_RS30155 WP_128798854.1 7523573..7524685(-) (pilT) [Corallococcus coralloides strain B035]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCTCTTCCGACCTCCACATCACCACCGGCTCGCCGCC
GCAGCTCCGCGTGGACGGTGAGCTCGTCCCGCTCAAGACGGCGCCGCTCACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAAGAGGAGAACGAGCTGGACCTGTCGTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCGAACATCTTCATGCAGCGCGGCGCCGTCGCCGGTGCGTTCCGCACGATTCCCTTCAAGATTCTGACGTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCCGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTGGGTGCGGACACGCGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACATCGTGCTCGTCGGTGAGCTCCGCGACCTGGAGACCATCGAAG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCCCCGTACCAGCAGCCCCAGGTCCGCGCGCAGCTGTCCTTCGTGCTGGAGGGCGTGATGAGCCA
GGCGCTCGTCGCCAAGCAGGGCGCCCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCCTCCATGCAGGTGGGGCAGGCGAAGTACGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCCCTTCTGGCGCGCCGGCTCATCTCCCAGGACGAGGCCTTCGGCCGTTCCTCCGACCCGGAGGAGCT
GCGCAACATCCTCGCCACGGGCGGTGGGCCGGGTGGAGCCCAGCGGCCGGCCGGGGGAGCGGGCGCCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

54.03

90.541

0.489
  pilT Legionella pneumophila strain Lp02

54.03

90.541

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.725

98.378

0.489
  pilT Pseudomonas stutzeri DSM 10701

51.884

93.243

0.484
  pilT Pseudomonas aeruginosa PAK

51.884

93.243

0.484
  pilT Acinetobacter baumannii strain A118

51.304

93.243

0.478
  pilT Acinetobacter baumannii D1279779

51.304

93.243

0.478
  pilT Acinetobacter baylyi ADP1

51.014

93.243

0.476
  pilT Acinetobacter nosocomialis M2

51.014

93.243

0.476
  pilT Vibrio cholerae strain A1552

48.204

90.27

0.435
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.204

90.27

0.435
  pilT Neisseria meningitidis 8013

44.282

92.162

0.408
  pilT Neisseria gonorrhoeae MS11

44.282

92.162

0.408
  pilU Vibrio cholerae strain A1552

42.776

95.405

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.449

96.216

0.389
  pilU Acinetobacter baylyi ADP1

39.769

93.784

0.373

Multiple sequence alignment