Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NX80_RS04205 Genome accession   NZ_CP034653
Coordinates   939297..939713 (+) Length   138 a.a.
NCBI ID   WP_010375211.1    Uniprot ID   -
Organism   Xanthomonas vasicola pv. arecae strain NCPPB 2649     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934297..944713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX80_RS23105 - 935520..935786 (-) 267 WP_193588613.1 hypothetical protein -
  NX80_RS04190 (NX80_004190) coaE 936182..936793 (-) 612 WP_017117847.1 dephospho-CoA kinase -
  NX80_RS04195 (NX80_004195) - 936807..937670 (-) 864 WP_017117848.1 A24 family peptidase -
  NX80_RS04200 (NX80_004200) pilC 937677..938933 (-) 1257 WP_026113135.1 type II secretion system F family protein Machinery gene
  NX80_RS04205 (NX80_004205) pilA/pilAI 939297..939713 (+) 417 WP_010375211.1 pilin Machinery gene
  NX80_RS04210 (NX80_004210) - 939720..940220 (+) 501 WP_322788741.1 pilin -
  NX80_RS04215 (NX80_004215) pilB 940285..942018 (+) 1734 WP_047699621.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NX80_RS04220 (NX80_004220) pilR 942488..943942 (-) 1455 WP_047699622.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14370.67 Da        Isoelectric Point: 8.4815

>NTDB_id=332125 NX80_RS04205 WP_010375211.1 939297..939713(+) (pilA/pilAI) [Xanthomonas vasicola pv. arecae strain NCPPB 2649]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQMAAALAEITPGKTQYEVMVNEGKAASITADLIGLKTSNRCTI
STPNPGETGAITCALLNSSPKLGSASISWTRAADGTWTCSTTLATADKLKYAPSTCQG

Nucleotide


Download         Length: 417 bp        

>NTDB_id=332125 NX80_RS04205 WP_010375211.1 939297..939713(+) (pilA/pilAI) [Xanthomonas vasicola pv. arecae strain NCPPB 2649]
ATGAAGAAGCAGCAAGGTTTTACATTGATCGAACTGATGATTGTGGTCGCCATCATTGCCATTCTGGCAGCGATTGCGCT
GCCGGCGTATCAAAACTATGTGGCCAAATCGCAAATGGCTGCAGCCTTGGCTGAGATCACTCCCGGCAAGACTCAGTACG
AAGTGATGGTTAACGAAGGCAAGGCGGCCTCTATCACTGCGGATCTCATCGGTCTCAAGACATCCAACCGTTGCACGATT
AGCACCCCGAATCCGGGTGAGACTGGCGCTATCACGTGTGCCTTGTTGAATTCCAGTCCGAAACTTGGCAGCGCTTCTAT
CTCCTGGACCCGAGCTGCGGATGGTACGTGGACATGTTCGACCACGTTGGCCACTGCTGATAAGCTTAAGTATGCTCCTT
CGACCTGCCAAGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

49.275

100

0.493
  pilA Acinetobacter baumannii strain A118

47.183

100

0.486
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.985

99.275

0.457
  pilA Pseudomonas aeruginosa PAK

43.796

99.275

0.435
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.69

100

0.428
  pilA Vibrio cholerae strain A1552

40.69

100

0.428
  pilA Vibrio cholerae C6706

40.69

100

0.428
  comP Acinetobacter baylyi ADP1

38.411

100

0.42
  pilA/pilA1 Eikenella corrodens VA1

36.667

100

0.399
  pilA Vibrio parahaemolyticus RIMD 2210633

40.909

95.652

0.391
  pilA2 Legionella pneumophila strain ERS1305867

36.806

100

0.384
  pilA2 Legionella pneumophila str. Paris

37.063

100

0.384
  pilE Neisseria gonorrhoeae MS11

32.704

100

0.377

Multiple sequence alignment