Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NX80_RS04215 Genome accession   NZ_CP034653
Coordinates   940285..942018 (+) Length   577 a.a.
NCBI ID   WP_047699621.1    Uniprot ID   -
Organism   Xanthomonas vasicola pv. arecae strain NCPPB 2649     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 935285..947018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX80_RS23105 - 935520..935786 (-) 267 WP_193588613.1 hypothetical protein -
  NX80_RS04190 (NX80_004190) coaE 936182..936793 (-) 612 WP_017117847.1 dephospho-CoA kinase -
  NX80_RS04195 (NX80_004195) - 936807..937670 (-) 864 WP_017117848.1 A24 family peptidase -
  NX80_RS04200 (NX80_004200) pilC 937677..938933 (-) 1257 WP_026113135.1 type II secretion system F family protein Machinery gene
  NX80_RS04205 (NX80_004205) pilA/pilAI 939297..939713 (+) 417 WP_010375211.1 pilin Machinery gene
  NX80_RS04210 (NX80_004210) - 939720..940220 (+) 501 WP_322788741.1 pilin -
  NX80_RS04215 (NX80_004215) pilB 940285..942018 (+) 1734 WP_047699621.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NX80_RS04220 (NX80_004220) pilR 942488..943942 (-) 1455 WP_047699622.1 sigma-54 dependent transcriptional regulator Regulator
  NX80_RS04225 (NX80_004225) - 944207..945820 (-) 1614 WP_017117852.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62789.88 Da        Isoelectric Point: 5.6039

>NTDB_id=332126 NX80_RS04215 WP_047699621.1 940285..942018(+) (pilB) [Xanthomonas vasicola pv. arecae strain NCPPB 2649]
MNVSASANLVGITGIARRLVQDGALEESVARSAMDQAASAKIPLPQWFSEKKLVSAAQLAAANAVEFGMPLIDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDDEMGDLDVSTGDEDMGAGGDSGVDARGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPEQIANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=332126 NX80_RS04215 WP_047699621.1 940285..942018(+) (pilB) [Xanthomonas vasicola pv. arecae strain NCPPB 2649]
ATGAACGTGAGTGCATCTGCAAATTTAGTTGGTATTACCGGGATTGCGCGTCGTCTAGTCCAAGATGGCGCCCTTGAAGA
GTCTGTAGCACGGTCCGCTATGGATCAAGCTGCCTCGGCGAAAATTCCACTTCCACAGTGGTTTTCTGAAAAGAAGCTAG
TCTCAGCTGCTCAGTTGGCTGCTGCCAACGCGGTCGAGTTCGGCATGCCGTTGATCGACGTATCAGTGTTCGATGCTAGC
CAGAACGCCGTCAAGCTGGTCAGCGAAGAGTTGCTCCAAAAGCACCAAGTGCTGCCGCTGTTCAAGCGTGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCAACCCAAACCCGGGCGCTGGATGACATTAAGTTCCATACGAACTTGGTAGTTGAGGCCA
TTCTTGTTGACGAGGATCAGATCCGTCGGACACTAGAACAGTGGCAGGCCAGCAACGCGTCTTTGGGTTCGTCGCTTGGT
AATGACGACGATGAGATGGGGGACTTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAGGGGCGACGACACGCCGGTGGTGAAGTTCGTCAATAAGATGTTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGATTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCAAAGGCGCCG
GTGAAGCTCAATCAGCGCATCGCGGCGCGGTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGAGTCAGCACCTTGCCCACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCTGACCAGCAGAAG
CTGTTCCTGGATGCGATCCATAAGCCGTATGGCATGGTGCTGGTGACCGGGCCTACGGGCTCGGGTAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGGAACATTTCCACGGCAGAGGATCCGGTCGAAATTCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTTGCCGCTGCGCTGCGTTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCATATGGT
GCTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAATATGGGGATCGCGCCTTACAACATCA
CCTCGTCGGTAACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGTCGACGTTGCCG
GACAACGCATTGCTGGCGGAAGGTTTCACGCCCGAGCAAATTGCGAACATGGAGCTCTACGAAGCCGTAGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCGGCGCAGAAAATCGGTATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCACGGCGTGACCAGCCTGGCTGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.44

0.549
  pilB Acinetobacter baylyi ADP1

54.766

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio cholerae strain A1552

49.002

95.494

0.468
  pilB Vibrio campbellii strain DS40M4

46.397

98.614

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444

Multiple sequence alignment