Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EIY89_RS06290 Genome accession   NZ_CP034246
Coordinates   1404476..1405513 (+) Length   345 a.a.
NCBI ID   WP_025009461.1    Uniprot ID   A0A1S2TIJ8
Organism   Shewanella algae strain VGH117     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1399476..1410513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EIY89_RS06255 (EIY89_06255) hemW 1399476..1400612 (-) 1137 WP_144159112.1 radical SAM family heme chaperone HemW -
  EIY89_RS06260 (EIY89_06260) rdgB 1400612..1401214 (-) 603 WP_025009467.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  EIY89_RS06265 (EIY89_06265) - 1401434..1401868 (-) 435 WP_025887551.1 DUF4426 domain-containing protein -
  EIY89_RS06270 (EIY89_06270) yggU 1401931..1402218 (-) 288 WP_037467122.1 DUF167 family protein YggU -
  EIY89_RS06275 (EIY89_06275) - 1402218..1402769 (-) 552 WP_044733985.1 YggT family protein -
  EIY89_RS06280 (EIY89_06280) proC 1402793..1403611 (-) 819 WP_144159109.1 pyrroline-5-carboxylate reductase -
  EIY89_RS06285 (EIY89_06285) - 1403744..1404439 (-) 696 WP_190535113.1 YggS family pyridoxal phosphate-dependent enzyme -
  EIY89_RS06290 (EIY89_06290) pilT 1404476..1405513 (+) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  EIY89_RS06295 (EIY89_06295) pilU 1405523..1406635 (+) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EIY89_RS06300 (EIY89_06300) - 1406760..1407869 (+) 1110 WP_052678495.1 hypothetical protein -
  EIY89_RS06305 (EIY89_06305) - 1407909..1408490 (+) 582 WP_190535116.1 glutathione peroxidase -
  EIY89_RS06310 (EIY89_06310) hemH 1408525..1409538 (+) 1014 WP_025009459.1 ferrochelatase -
  EIY89_RS06315 (EIY89_06315) - 1409714..1410448 (+) 735 WP_071237900.1 tryptophan 2,3-dioxygenase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.97 Da        Isoelectric Point: 6.8739

>NTDB_id=330189 EIY89_RS06290 WP_025009461.1 1404476..1405513(+) (pilT) [Shewanella algae strain VGH117]
MEITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINVPALDHQGVHRLVYDIMNDKQRKDYEEFLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILTLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QNLVNRGLISREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=330189 EIY89_RS06290 WP_025009461.1 1404476..1405513(+) (pilT) [Shewanella algae strain VGH117]
ATGGAAATCACTGAATTATTGGCCTTTAGTGTAAAGCATAACGCTTCGGATCTGCACCTGTCGGCCGGTGTGCCTCCCAT
GATACGCGTCGACGGCGAGGTCAGAAAGATTAATGTCCCTGCGCTTGATCACCAAGGGGTTCACAGACTTGTCTATGACA
TAATGAATGACAAGCAGCGTAAGGATTATGAAGAGTTTCTGGAAATCGACTTTTCCTTTGAAGTTCCCAATCTGGCGCGT
TTTCGTGTCAATGCCTTCAATCAGTCCCGCGGCGCCGCCGCAGTTTTTCGTACTATTCCCAGCGACATTTTGACTTTGGA
ACAACTGGGCGCGCCGGAAATCTTCAAGAAAATCGCTTCATATCCTCGCGGCCTGGTGTTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACCACCCTGGCGGCGATGATAGATTACGTCAATGAGAACCGCCACGATCATATTCTCACCATTGAAGAC
CCTATCGAATTCGTGCACCAAAACAAGCAATGCCTTATCAACCAGCGGGAAGTGCACCGCCATACCCACAGCTTTAACGC
TGCCCTCAGAAGCGCCCTGCGTGAAGACCCCGATGTCATCCTGGTCGGTGAGATGCGGGATTTGGAAACCATACGCCTGG
CGATGACCGCGGCAGAAACCGGCCACCTGGTGTTCGGTACCTTGCACACCACCTCGGCGGCCAAGACCATTGACCGTGTG
GTTGACGTATTCCCCGCAGGCGAGAAGGACATGGTGCGTACCATGTTGTCTGAGTCGTTGCAGGCGGTTATCTCCCAGAC
CCTGATCAAGAAAGTTGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGATGGGAACCCCGGCTATCCGAAACCTTATCC
GTGAGGACAAGGTGGCACAGATGTATTCTGCTATCCAAACCGGTATGGCCCACGGCATGCAAACCTTGGAGCAGAGCCTG
CAGAACCTGGTGAATCGCGGCTTGATCAGTCGGGAAGATGCCATGGCCAAGAGCTCCAACAAACAAGCAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2TIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.871

99.13

0.812
  pilT Acinetobacter baumannii D1279779

81.871

99.13

0.812
  pilT Acinetobacter baumannii strain A118

81.871

99.13

0.812
  pilT Acinetobacter baylyi ADP1

78.426

99.42

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment