Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NAMH_RS05640 Genome accession   NC_012115
Coordinates   1059432..1060499 (-) Length   355 a.a.
NCBI ID   WP_012663611.1    Uniprot ID   B9LA81
Organism   Nautilia profundicola AmH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1046659..1090049 1059432..1060499 within 0


Gene organization within MGE regions


Location: 1046659..1090049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAMH_RS05580 (NAMH_1130) - 1048131..1049936 (-) 1806 WP_015902349.1 primosomal protein N' -
  NAMH_RS09005 (NAMH_1132) - 1049933..1050355 (-) 423 WP_015902077.1 type II secretion system protein -
  NAMH_RS09400 (NAMH_1133) - 1050402..1050908 (-) 507 WP_012663859.1 type II secretion system protein -
  NAMH_RS05595 (NAMH_1134) - 1051095..1051301 (-) 207 WP_015901927.1 hypothetical protein -
  NAMH_RS05600 (NAMH_1135) - 1051298..1051519 (-) 222 WP_015902682.1 hypothetical protein -
  NAMH_RS05605 (NAMH_1136) uvrB 1051587..1053617 (+) 2031 WP_015902464.1 excinuclease ABC subunit UvrB -
  NAMH_RS05610 (NAMH_1137) - 1053627..1054067 (+) 441 WP_012663995.1 hypothetical protein -
  NAMH_RS05615 (NAMH_1138) - 1054085..1054624 (+) 540 WP_012663737.1 gamma carbonic anhydrase family protein -
  NAMH_RS05620 (NAMH_1139) - 1054628..1056793 (+) 2166 WP_015902343.1 endonuclease MutS2 -
  NAMH_RS05625 (NAMH_1140) - 1057072..1057596 (-) 525 WP_015902684.1 peptidylprolyl isomerase -
  NAMH_RS05630 (NAMH_1141) trxC 1057874..1058314 (+) 441 WP_015901897.1 thioredoxin TrxC -
  NAMH_RS05635 (NAMH_1142) queA 1058370..1059413 (-) 1044 WP_049751153.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  NAMH_RS05640 (NAMH_1143) pilT 1059432..1060499 (-) 1068 WP_012663611.1 type IV pilus twitching motility protein PilT Machinery gene
  NAMH_RS05645 (NAMH_1144) gatC 1060510..1060791 (-) 282 WP_012663858.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  NAMH_RS05650 (NAMH_1145) hemL 1060844..1062106 (+) 1263 WP_015902126.1 glutamate-1-semialdehyde 2,1-aminomutase -
  NAMH_RS05655 (NAMH_1146) - 1062106..1062420 (+) 315 WP_015901760.1 AtpZ/AtpI family protein -
  NAMH_RS05660 (NAMH_1147) - 1062422..1063666 (-) 1245 WP_015902245.1 type II secretion system F family protein -
  NAMH_RS05665 (NAMH_1148) - 1063663..1065300 (-) 1638 WP_015902765.1 GspE/PulE family protein -
  NAMH_RS05670 (NAMH_1149) - 1065297..1065842 (-) 546 WP_015902174.1 hypothetical protein -
  NAMH_RS05675 (NAMH_1150) ilvC 1065917..1066936 (+) 1020 WP_012663874.1 ketol-acid reductoisomerase -
  NAMH_RS05680 (NAMH_1151) minD 1067004..1067813 (+) 810 WP_015902584.1 septum site-determining protein MinD -
  NAMH_RS05685 (NAMH_1152) minE 1067810..1068043 (+) 234 WP_015902219.1 cell division topological specificity factor MinE -
  NAMH_RS05690 (NAMH_1153) - 1068040..1069032 (+) 993 WP_012663857.1 divergent polysaccharide deacetylase family protein -
  NAMH_RS05695 (NAMH_1154) - 1069029..1069736 (+) 708 WP_015902100.1 DNA-processing protein DprA -
  NAMH_RS05700 (NAMH_1155) - 1069753..1070271 (+) 519 WP_012663546.1 DNA-deoxyinosine glycosylase -
  NAMH_RS05705 (NAMH_1157) ruvX 1070244..1070636 (+) 393 WP_012663981.1 Holliday junction resolvase RuvX -
  NAMH_RS09015 (NAMH_1156) - 1070610..1072022 (-) 1413 WP_015902619.1 GGDEF domain-containing protein -
  NAMH_RS05715 (NAMH_1158) - 1072015..1072842 (-) 828 WP_015902710.1 ketopantoate reductase family protein -
  NAMH_RS05720 (NAMH_1159) trmA 1072988..1074085 (-) 1098 WP_015901975.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
  NAMH_RS05725 (NAMH_1160) fliP 1074142..1074870 (-) 729 WP_015902261.1 flagellar type III secretion system pore protein FliP -
  NAMH_RS05730 (NAMH_1161) - 1074878..1075654 (-) 777 WP_012663690.1 OmpA family protein -
  NAMH_RS05735 (NAMH_1162) - 1075663..1076430 (-) 768 WP_015902365.1 motility protein A -
  NAMH_RS05740 (NAMH_1163) glmU 1076478..1077761 (+) 1284 WP_015901740.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  NAMH_RS05745 (NAMH_1164) coaBC 1077758..1078900 (+) 1143 WP_015902183.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  NAMH_RS05750 (NAMH_1165) - 1078881..1079576 (+) 696 WP_012663678.1 hypothetical protein -
  NAMH_RS05755 (NAMH_1166) - 1079578..1080240 (+) 663 WP_012664008.1 di-trans,poly-cis-decaprenylcistransferase -
  NAMH_RS05760 (NAMH_1167) - 1080291..1081037 (+) 747 WP_015902507.1 prepilin peptidase -
  NAMH_RS05765 (NAMH_1168) - 1081030..1082001 (+) 972 WP_015902432.1 LptF/LptG family permease -
  NAMH_RS05770 (NAMH_1170) truA 1082010..1082711 (+) 702 WP_228368714.1 tRNA pseudouridine(38-40) synthase TruA -
  NAMH_RS05775 (NAMH_1169) - 1082677..1083378 (-) 702 WP_015901805.1 polysaccharide lyase family 7 protein -
  NAMH_RS05780 (NAMH_1171) - 1083505..1083843 (-) 339 WP_015902517.1 hypothetical protein -
  NAMH_RS05785 (NAMH_1172) - 1083861..1084148 (-) 288 WP_012663757.1 hypothetical protein -
  NAMH_RS05790 (NAMH_1173) - 1084225..1085130 (+) 906 WP_015902294.1 tyrosine-type recombinase/integrase -
  NAMH_RS05795 (NAMH_1174) - 1085232..1085555 (+) 324 WP_012663653.1 hypothetical protein -
  NAMH_RS05800 (NAMH_1175) - 1085555..1085779 (+) 225 WP_015902102.1 hypothetical protein -
  NAMH_RS05805 (NAMH_1176) - 1085841..1086026 (+) 186 WP_012663768.1 hypothetical protein -
  NAMH_RS05810 (NAMH_1177) - 1086033..1086455 (+) 423 WP_015902226.1 hypothetical protein -
  NAMH_RS05815 (NAMH_1178) - 1086733..1088247 (+) 1515 WP_012663644.1 hypothetical protein -
  NAMH_RS05820 (NAMH_1179) - 1088247..1088669 (+) 423 WP_015902020.1 hypothetical protein -
  NAMH_RS05825 (NAMH_1180) - 1088671..1089840 (+) 1170 WP_015902735.1 zonular occludens toxin domain-containing protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39907.21 Da        Isoelectric Point: 7.2368

>NTDB_id=33008 NAMH_RS05640 WP_012663611.1 1059432..1060499(-) (pilT) [Nautilia profundicola AmH]
MALTYSLEQLLRSIKDYKASDLHLNVNAEPMLRIDGKLTPLNLPKLTKEDVIELCYSVLTEKQKAALEDQLELDFSFEIP
KVARFRANYYFERENLAAAYRIIPEKPFTLEELHAPAIFKRIVRREKGLILVTGPTGSGKSTTLAAMINEINEAFSKHII
TIEDPIEFVHEHKKSLISQREVGRDTKAFLNALRASLREDPDVILIGEMRDKETIGAAITAAETGHLVFATLHTNSAVQT
INRIVNVFPANEQDQIRTQLSMAILAVISQSLLPKIGGGRIAAHEIMINNPAIANLIRENKIPQIYSQMQLNQGATGMQT
MNQVLAEYVRRHIIDKETAMAYTTKPEELRKLIGV

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=33008 NAMH_RS05640 WP_012663611.1 1059432..1060499(-) (pilT) [Nautilia profundicola AmH]
ATGGCACTAACATATTCACTTGAACAACTTTTAAGAAGTATAAAAGATTACAAAGCATCGGATTTGCATCTTAATGTAAA
TGCTGAACCGATGCTAAGAATTGATGGTAAGTTAACACCACTAAACCTTCCTAAACTTACCAAAGAAGATGTAATTGAGC
TTTGTTATTCTGTTTTAACTGAAAAACAAAAGGCTGCATTAGAAGATCAATTAGAACTTGACTTTTCTTTTGAAATTCCG
AAAGTTGCGAGATTTAGGGCAAATTACTATTTTGAAAGAGAAAATTTAGCTGCTGCATACAGAATTATTCCTGAAAAACC
TTTTACATTAGAAGAACTTCACGCACCTGCAATATTTAAAAGAATAGTAAGAAGGGAAAAAGGGCTTATTCTTGTAACGG
GTCCAACAGGTAGCGGTAAATCTACAACACTTGCTGCAATGATAAATGAAATAAACGAAGCTTTTTCAAAACATATTATT
ACAATTGAAGATCCTATTGAGTTTGTGCATGAGCATAAAAAATCACTTATTTCCCAAAGAGAAGTCGGAAGAGATACAAA
AGCGTTTTTAAACGCTCTAAGAGCTTCACTTAGGGAAGACCCGGATGTGATCTTAATCGGAGAGATGAGAGATAAAGAAA
CTATCGGTGCAGCAATTACTGCAGCTGAAACAGGGCACCTTGTATTTGCTACATTGCATACTAACTCTGCCGTTCAGACA
ATCAACAGAATCGTAAACGTTTTCCCTGCAAACGAACAGGATCAAATAAGAACTCAGCTTTCTATGGCTATACTTGCAGT
TATTTCACAAAGCTTACTTCCTAAAATAGGAGGAGGTAGAATTGCCGCTCATGAAATTATGATTAATAACCCGGCAATTG
CAAACCTTATCCGTGAAAATAAAATTCCTCAGATTTATTCGCAAATGCAGCTTAATCAAGGTGCAACCGGAATGCAGACT
ATGAATCAGGTTCTCGCTGAATATGTTAGACGCCATATTATAGATAAAGAAACTGCAATGGCTTATACTACTAAACCGGA
AGAGCTTAGAAAACTTATAGGCGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9LA81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

55.685

96.62

0.538
  pilT Acinetobacter baumannii strain A118

55.685

96.62

0.538
  pilT Acinetobacter nosocomialis M2

55.685

96.62

0.538
  pilT Acinetobacter baylyi ADP1

56.156

93.803

0.527
  pilT Neisseria meningitidis 8013

57.585

90.986

0.524
  pilT Neisseria gonorrhoeae MS11

57.276

90.986

0.521
  pilT Pseudomonas aeruginosa PAK

54.985

93.239

0.513
  pilT Legionella pneumophila strain Lp02

54.819

93.521

0.513
  pilT Legionella pneumophila strain ERS1305867

54.819

93.521

0.513
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.381

93.239

0.507
  pilT Vibrio cholerae strain A1552

54.381

93.239

0.507
  pilT Pseudomonas stutzeri DSM 10701

53.776

93.239

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.875

99.437

0.476
  pilU Acinetobacter baylyi ADP1

41.691

96.62

0.403
  pilU Vibrio cholerae strain A1552

39.943

98.028

0.392
  pilU Pseudomonas stutzeri DSM 10701

38.793

98.028

0.38

Multiple sequence alignment