Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EH164_RS03470 Genome accession   NZ_CP034225
Coordinates   747133..748113 (+) Length   326 a.a.
NCBI ID   WP_124967303.1    Uniprot ID   -
Organism   Kosakonia sp. CCTCC M2018092     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742133..753113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EH164_RS03440 (EH164_03440) wzz(fepE) 742621..743727 (+) 1107 WP_124967298.1 LPS O-antigen length regulator Wzz(fepE) -
  EH164_RS03445 (EH164_03445) hemW 743808..744944 (-) 1137 WP_124967299.1 radical SAM family heme chaperone HemW -
  EH164_RS03450 (EH164_03450) - 744937..745530 (-) 594 WP_124967300.1 XTP/dITP diphosphatase -
  EH164_RS03455 (EH164_03455) yggU 745539..745829 (-) 291 WP_042713571.1 DUF167 family protein YggU -
  EH164_RS03460 (EH164_03460) - 745826..746392 (-) 567 WP_124967301.1 YggT family protein -
  EH164_RS03465 (EH164_03465) - 746411..747115 (-) 705 WP_124967302.1 YggS family pyridoxal phosphate-dependent enzyme -
  EH164_RS03470 (EH164_03470) pilT 747133..748113 (+) 981 WP_124967303.1 type IV pilus twitching motility protein PilT Machinery gene
  EH164_RS03475 (EH164_03475) - 748110..750368 (-) 2259 WP_124967304.1 FAD-dependent oxidoreductase -
  EH164_RS03480 (EH164_03480) - 750436..751128 (+) 693 WP_124967305.1 IclR family transcriptional regulator -
  EH164_RS03485 (EH164_03485) ruvX 751260..751676 (-) 417 WP_023480823.1 Holliday junction resolvase RuvX -
  EH164_RS03490 (EH164_03490) - 751676..752239 (-) 564 WP_124967306.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35240.10 Da        Isoelectric Point: 7.2887

>NTDB_id=329880 EH164_RS03470 WP_124967303.1 747133..748113(+) (pilT) [Kosakonia sp. CCTCC M2018092]
MDIEEIVALSVKHNVSDLHLCSGSPPRWRQHGQLTAAPFEPPDIIALLSGQLTSLQQQQWRETRQVDFALTTAGGRRLRA
GAFARHGGISLALRLLPQRCPQLSDISAPAALTELLARENGLILVTGATGSGKSTTLAAMVQHLNQQAGGHIVTLEDPIE
FVHQSGNSLIQQREIGQHCASFADGLRAALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLQAVLAQRLEADKQGGRVALFELLINTPAVANLIREGKTHQLPGVMQTGQQQGMQTFAQSQQQR
VREGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=329880 EH164_RS03470 WP_124967303.1 747133..748113(+) (pilT) [Kosakonia sp. CCTCC M2018092]
ATGGATATAGAAGAAATAGTGGCGCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGTGGTTCTCCACCGCG
CTGGCGACAGCACGGGCAGCTTACCGCTGCGCCTTTTGAGCCGCCCGACATCATCGCACTGCTCAGCGGGCAGCTTACCT
CCCTGCAACAGCAGCAGTGGCGCGAAACGAGACAGGTGGATTTTGCGCTCACCACAGCTGGCGGCAGGCGACTGCGCGCA
GGCGCGTTTGCCCGCCACGGCGGCATATCATTAGCGCTGCGCCTGTTGCCACAGCGCTGCCCGCAGCTTAGCGATATCTC
TGCTCCGGCGGCGCTGACTGAACTGCTGGCGCGGGAGAACGGTCTGATCCTCGTCACCGGCGCGACCGGTAGCGGTAAAT
CGACAACCCTTGCGGCGATGGTGCAACACCTCAATCAACAGGCCGGGGGGCATATTGTGACGCTGGAAGATCCCATTGAG
TTCGTGCACCAGAGCGGCAACAGCCTGATCCAGCAGCGCGAGATAGGTCAACACTGCGCCTCGTTTGCCGACGGGCTGCG
TGCAGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGCGAGTTGCGCGATAGCGAGACGATCCGCCTGGCCCTGACCG
CCGCCGAAACCGGGCATCTGGTGCTGGCGACCCTGCACACGCGCGGCGCGGCGCAGGCCGTCGAGCGGCTGGTGGACAGC
TTTCCGGCACAAGAAAAGGAGCCGGTGCGCAGCCAGCTGGCGGGCAGCTTGCAGGCGGTGCTGGCGCAGAGGCTGGAGGC
GGATAAGCAGGGCGGGCGCGTGGCGCTGTTTGAGTTGCTGATTAACACCCCGGCGGTGGCGAACCTTATTCGCGAAGGCA
AAACCCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAGCAGGGGATGCAGACCTTCGCGCAGAGCCAGCAGCAGCGG
GTGCGCGAAGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

46.789

100

0.469
  pilT Legionella pneumophila strain ERS1305867

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.468

96.933

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.912

97.546

0.448
  pilT Acinetobacter baylyi ADP1

44.343

100

0.445
  pilU Vibrio cholerae strain A1552

40.122

100

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386

Multiple sequence alignment