Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ATHE_RS09450 Genome accession   NC_012034
Coordinates   1990342..1991397 (-) Length   351 a.a.
NCBI ID   WP_015908266.1    Uniprot ID   B9MKW3
Organism   Caldicellulosiruptor bescii DSM 6725     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1985342..1996397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATHE_RS09415 (Athe_1876) - 1985892..1986464 (-) 573 WP_015908259.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ATHE_RS09420 (Athe_1877) - 1986461..1987015 (-) 555 WP_015908260.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ATHE_RS09425 (Athe_1878) - 1987005..1987295 (-) 291 WP_013429858.1 late competence development ComFB family protein -
  ATHE_RS09430 (Athe_1879) - 1987303..1988079 (-) 777 WP_015908262.1 A24 family peptidase -
  ATHE_RS09435 (Athe_1880) - 1988132..1988569 (-) 438 WP_015908263.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ATHE_RS09440 (Athe_1881) - 1988603..1989019 (-) 417 WP_041727170.1 type II secretion system protein -
  ATHE_RS09445 (Athe_1882) - 1989060..1990271 (-) 1212 WP_015908265.1 type II secretion system F family protein -
  ATHE_RS09450 (Athe_1883) pilT 1990342..1991397 (-) 1056 WP_015908266.1 type IV pilus twitching motility protein PilT Machinery gene
  ATHE_RS09455 (Athe_1884) - 1991451..1993163 (-) 1713 WP_015908267.1 GspE/PulE family protein -
  ATHE_RS09460 (Athe_1885) - 1993184..1996384 (-) 3201 WP_015908268.1 response regulator -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39695.80 Da        Isoelectric Point: 7.2029

>NTDB_id=32972 ATHE_RS09450 WP_015908266.1 1990342..1991397(-) (pilT) [Caldicellulosiruptor bescii DSM 6725]
MDINSILKEAFLKEASDIHITPGVPPIYRIHGRLVRTDDSILTPEMVEEFVRQITNENQFKILEQKGEIDFSYGIKGVSR
FRVNVYKQRGSYSIAFRIIPVNIPPFETLGLPPVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIINKERDVHIITLED
PIEYLHRHNKSIINQREIGSDTLSFADALRAALREDPDVILVGEMRDLETIAIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPYQQQQIRIQLSTVLQGVVSQQLLTRRDGKGRVVATEVMIVNPAIRNLIREAKTYQIQSIIQTHQRQGMITMEQS
LIDLYKRGFITREDAFNYATDFDFMQRLLSA

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=32972 ATHE_RS09450 WP_015908266.1 1990342..1991397(-) (pilT) [Caldicellulosiruptor bescii DSM 6725]
ATGGATATTAATTCAATTCTCAAAGAAGCATTTCTCAAGGAGGCTTCAGATATACATATTACACCAGGTGTTCCTCCAAT
TTATCGAATACACGGCAGATTAGTACGGACAGATGATTCAATTCTGACCCCTGAGATGGTGGAGGAGTTCGTGCGACAGA
TCACCAACGAAAACCAGTTTAAAATTCTTGAGCAGAAAGGTGAGATAGACTTTTCTTACGGCATAAAAGGTGTAAGCAGA
TTTAGAGTAAATGTTTATAAACAAAGAGGGTCATATTCTATAGCTTTTAGAATAATTCCAGTAAATATACCACCATTTGA
GACACTTGGTCTTCCACCAGTTTTGAAAGAATTTACAAAATTGAATAAAGGGCTTGTTTTAGTTACAGGTCCAACCGGTT
CGGGTAAGTCAACAACACTAGCATCGCTGATTGACATAATCAACAAAGAAAGAGATGTGCATATAATCACATTAGAGGAT
CCAATAGAGTATTTGCATAGACACAACAAGAGTATTATCAATCAAAGAGAGATAGGTAGTGACACGCTCAGCTTTGCAGA
CGCGCTGAGGGCGGCTTTGAGAGAAGATCCTGACGTAATCCTTGTTGGTGAGATGAGAGATTTGGAGACAATTGCAATAG
CTTTAACAGCTGCTGAAACAGGACACCTAGTGTTTTCCACACTTCACACAATTGGAGCGGCAAAGACAATAGACCGTATC
ATTGACGTTTTTCCACCGTATCAACAGCAGCAGATAAGAATTCAGCTATCAACTGTTTTGCAGGGAGTAGTGTCTCAGCA
GCTTTTGACCCGTCGTGATGGTAAAGGCAGGGTTGTTGCAACAGAGGTAATGATAGTAAATCCGGCCATAAGAAACCTCA
TTAGGGAGGCTAAAACGTATCAGATTCAATCAATTATTCAGACGCATCAGCGACAGGGCATGATAACAATGGAGCAGTCA
CTCATAGACTTGTACAAACGAGGGTTTATTACCCGTGAAGATGCGTTCAACTATGCTACTGACTTTGATTTTATGCAAAG
ACTGCTCAGTGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9MKW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.724

99.145

0.513
  pilT Neisseria meningitidis 8013

51.156

98.575

0.504
  pilT Acinetobacter nosocomialis M2

51.917

96.581

0.501
  pilT Acinetobacter baumannii D1279779

51.917

96.581

0.501
  pilT Acinetobacter baumannii strain A118

51.917

96.581

0.501
  pilT Legionella pneumophila strain ERS1305867

51.622

96.581

0.499
  pilT Legionella pneumophila strain Lp02

51.622

96.581

0.499
  pilT Neisseria gonorrhoeae MS11

50.435

98.291

0.496
  pilT Acinetobacter baylyi ADP1

52.424

94.017

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.796

95.157

0.493
  pilT Vibrio cholerae strain A1552

51.796

95.157

0.493
  pilT Pseudomonas aeruginosa PAK

51.045

95.442

0.487
  pilT Pseudomonas stutzeri DSM 10701

50.746

95.442

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.36

100

0.416
  pilU Acinetobacter baylyi ADP1

40.882

96.866

0.396
  pilU Vibrio cholerae strain A1552

38.395

99.43

0.382

Multiple sequence alignment