Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TGR7_RS14640 Genome accession   NC_011901
Coordinates   3062255..3063289 (-) Length   344 a.a.
NCBI ID   WP_012639454.1    Uniprot ID   B8GP71
Organism   Thioalkalivibrio sulfidiphilus HL-EbGr7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3057255..3068289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TGR7_RS14620 (Tgr7_2914) - 3057360..3058640 (+) 1281 WP_012639450.1 dihydroorotase -
  TGR7_RS14625 (Tgr7_2915) - 3058726..3059595 (+) 870 WP_012639451.1 dihydroorotate dehydrogenase electron transfer subunit -
  TGR7_RS14630 (Tgr7_2916) - 3059694..3060902 (-) 1209 WP_012639452.1 PilT/PilU family type 4a pilus ATPase -
  TGR7_RS14635 (Tgr7_2917) pilU 3061023..3062189 (-) 1167 WP_012639453.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TGR7_RS14640 (Tgr7_2918) pilT 3062255..3063289 (-) 1035 WP_012639454.1 type IV pilus twitching motility protein PilT Machinery gene
  TGR7_RS14645 (Tgr7_2919) - 3063522..3064232 (+) 711 WP_012639455.1 YggS family pyridoxal phosphate-dependent enzyme -
  TGR7_RS14650 (Tgr7_2920) proC 3064270..3065097 (+) 828 WP_012639456.1 pyrroline-5-carboxylate reductase -
  TGR7_RS14655 (Tgr7_2921) - 3065381..3065935 (+) 555 WP_012639457.1 YggT family protein -
  TGR7_RS14660 (Tgr7_2922) - 3066075..3066524 (+) 450 WP_012639458.1 DUF4426 domain-containing protein -
  TGR7_RS14665 (Tgr7_2923) - 3066565..3067242 (-) 678 WP_012639459.1 sulfite exporter TauE/SafE family protein -
  TGR7_RS14670 (Tgr7_2924) - 3067408..3067764 (+) 357 WP_012639460.1 HU family DNA-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38024.76 Da        Isoelectric Point: 6.8890

>NTDB_id=32753 TGR7_RS14640 WP_012639454.1 3062255..3063289(-) (pilT) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MDITQLLAFAVKNGASDLHLSAGLPPMIRVDGDVRRINVPALDHKAVHGMVYDIMNDKQRKDYEEFLETDFSFEIPGLAR
FRVNAFNHNRGAGAVFRTIPSKILTLEELNCPKIFEQISDYPRGIVLVTGPTGSGKSTTLAAMINHKNESEYGHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRAMLSESLRAVISQTLLKRIGGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTSQGMGMQTLDMSL
KELLAKGIISKEDARRKAANPDAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=32753 TGR7_RS14640 WP_012639454.1 3062255..3063289(-) (pilT) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGGACATTACGCAGCTGCTTGCGTTTGCGGTCAAGAATGGCGCCTCGGACCTGCACCTCTCCGCCGGGCTGCCGCCCAT
GATCCGCGTCGACGGCGACGTGCGCAGGATCAACGTACCCGCCCTGGACCACAAGGCTGTCCACGGCATGGTGTACGACA
TCATGAACGACAAGCAGCGCAAGGACTACGAGGAGTTCCTGGAAACGGACTTCTCCTTCGAGATCCCCGGCCTGGCGCGC
TTCCGTGTCAACGCCTTCAACCACAACCGCGGCGCCGGCGCGGTGTTCCGAACCATCCCGAGCAAGATCCTCACCCTCGA
AGAACTCAACTGCCCGAAGATCTTCGAGCAGATCTCCGACTACCCGCGCGGCATCGTGCTGGTCACCGGGCCCACCGGCT
CCGGCAAGTCCACCACCCTGGCCGCCATGATCAACCACAAGAACGAATCCGAGTACGGGCACATCCTCACCGTGGAGGAC
CCGATCGAGTTCGTGCACGAGAGCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCAACGA
GGCCCTGCGCTCGGCCCTGCGTGAGGACCCGGACACCATCCTGGTGGGCGAGATGCGCGATCTGGAGACCATCCGACTGG
CCCTGACCGCCGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCCGCCAAGACCATCGACCGTATC
GTGGACGTGTTCCCCGCCGCGGAGAAGGACATGGTGCGCGCCATGCTGTCGGAGTCCTTGCGGGCGGTGATCTCCCAGAC
CCTGCTCAAGCGCATCGGCGGCGGGCGCGTGGCGGCCCACGAGATCATGCTGGGCACCCCGGCCATCCGCAACCTGATCC
GTGAAAACAAGATCGCCCAGATGTACTCGGCCATCCAGACCAGCCAGGGCATGGGCATGCAGACCCTGGACATGAGTCTC
AAGGAGCTGCTCGCCAAGGGCATCATCAGCAAGGAAGACGCACGCCGCAAGGCCGCCAACCCGGACGCCCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8GP71

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.287

99.419

0.808
  pilT Acinetobacter baumannii D1279779

80.814

100

0.808
  pilT Acinetobacter baumannii strain A118

80.814

100

0.808
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

79.942

100

0.799
  pilT Pseudomonas stutzeri DSM 10701

80.409

99.419

0.799
  pilT Legionella pneumophila strain Lp02

73.824

98.837

0.73
  pilT Legionella pneumophila strain ERS1305867

73.824

98.837

0.73
  pilT Neisseria meningitidis 8013

68.406

100

0.686
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.585

99.419

0.503
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395

Multiple sequence alignment