Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TGR7_RS02270 Genome accession   NC_011901
Coordinates   490622..491692 (+) Length   356 a.a.
NCBI ID   WP_012637047.1    Uniprot ID   -
Organism   Thioalkalivibrio sulfidiphilus HL-EbGr7     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485622..496692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TGR7_RS02255 (Tgr7_0456) - 486562..487035 (+) 474 WP_012637043.1 hypothetical protein -
  TGR7_RS17710 (Tgr7_0457) - 487112..487780 (+) 669 WP_012637044.1 HEAT repeat domain-containing protein -
  TGR7_RS02260 (Tgr7_0458) - 487740..489521 (+) 1782 WP_012637045.1 HEAT repeat domain-containing protein -
  TGR7_RS02265 (Tgr7_0459) - 489521..490615 (+) 1095 WP_012637046.1 PilT/PilU family type 4a pilus ATPase -
  TGR7_RS02270 (Tgr7_0460) pilT 490622..491692 (+) 1071 WP_012637047.1 type IV pilus twitching motility protein PilT Machinery gene
  TGR7_RS02275 (Tgr7_0461) - 491783..492538 (-) 756 WP_012637048.1 hypothetical protein -
  TGR7_RS02280 (Tgr7_0462) - 492538..493095 (-) 558 WP_012637049.1 sigma-70 family RNA polymerase sigma factor -
  TGR7_RS02285 (Tgr7_0463) glnE 493712..496624 (+) 2913 WP_012637050.1 bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 38784.52 Da        Isoelectric Point: 7.2064

>NTDB_id=32734 TGR7_RS02270 WP_012637047.1 490622..491692(+) (pilT) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MKRVDALLTRMVEMDGSDLHMMAGDPPRVRVYGELKPIEEAPMSAESVSDALAEILPDHSRLVFEREDGADFAYEIPGLS
RFRVNMLRQINGIGAVFRGIPSRSLSLDDLGLPPVVSSLSMQRHGMVLVTGKTGSGKTTTLAAIVDDVNARRKGHIITIE
DPIEFIHERKRSLISQRQVGLHTPSFSSALRSALREDPDVILVGEMRDLETISLAVTAAETGILVLGTLHTSGAASTVDR
IINTFPAQKQAQIRTMLSTSLRGVISQQLARRADGKGRVAAIEILVNTPAVSNLIREGRTEQLESTMQSGALVGMQTMDS
ALRRLLDAGTITGREAYEKAISKAEFETVRHEGVKR

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=32734 TGR7_RS02270 WP_012637047.1 490622..491692(+) (pilT) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGAAACGCGTCGATGCGCTGCTGACCCGCATGGTGGAAATGGATGGCTCCGACCTGCACATGATGGCGGGCGATCCGCC
CCGGGTACGTGTCTACGGGGAGCTCAAGCCCATTGAAGAGGCACCCATGAGCGCCGAGTCAGTCTCCGATGCCCTGGCGG
AGATACTGCCGGATCATTCACGGCTTGTCTTCGAGCGGGAAGATGGCGCCGATTTCGCCTACGAGATCCCGGGGCTGTCA
CGATTCCGGGTCAACATGCTGCGTCAGATCAACGGCATCGGCGCGGTGTTTCGCGGCATCCCTTCCCGCTCCCTGAGCCT
GGATGATCTGGGGCTTCCACCGGTAGTCTCCAGCCTGAGCATGCAGCGACACGGCATGGTCCTGGTCACCGGCAAGACGG
GCTCGGGTAAGACCACCACGCTTGCGGCCATCGTCGATGACGTCAATGCCCGTCGCAAGGGCCACATCATCACCATTGAA
GACCCCATCGAGTTCATCCATGAACGCAAACGCTCGCTGATCAGCCAGCGCCAGGTGGGGCTGCACACGCCAAGCTTCTC
CAGTGCCTTGCGCTCGGCGCTGCGCGAGGATCCGGATGTCATTCTGGTGGGTGAGATGCGCGATCTCGAGACCATCAGCC
TGGCGGTGACTGCCGCCGAGACCGGTATCCTCGTGCTCGGAACCCTGCATACCAGCGGAGCGGCCTCCACGGTGGACCGC
ATCATCAACACCTTCCCGGCACAGAAACAGGCGCAGATCCGCACCATGCTCTCCACCTCCCTGCGTGGCGTCATCTCACA
GCAGCTGGCGCGGCGCGCCGACGGCAAGGGCCGCGTGGCGGCCATCGAGATCCTGGTGAACACCCCGGCGGTCTCGAATC
TCATTCGCGAGGGGCGTACCGAGCAGCTGGAGAGCACCATGCAGAGCGGTGCCCTGGTGGGCATGCAGACCATGGACTCG
GCCCTGCGCCGGCTGCTGGATGCGGGCACCATCACCGGGCGGGAGGCCTACGAGAAGGCCATCAGCAAGGCGGAGTTCGA
GACCGTGCGCCACGAGGGTGTAAAGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

52.187

96.348

0.503
  pilT Pseudomonas stutzeri DSM 10701

51.765

95.506

0.494
  pilT Pseudomonas aeruginosa PAK

51.929

94.663

0.492
  pilT Neisseria meningitidis 8013

51.479

94.944

0.489
  pilT Neisseria gonorrhoeae MS11

51.183

94.944

0.486
  pilT Legionella pneumophila strain ERS1305867

50.588

95.506

0.483
  pilT Legionella pneumophila strain Lp02

50.588

95.506

0.483
  pilT Acinetobacter baumannii D1279779

50.294

95.506

0.48
  pilT Acinetobacter nosocomialis M2

50.294

95.506

0.48
  pilT Acinetobacter baumannii strain A118

50.294

95.506

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.051

93.539

0.478
  pilT Vibrio cholerae strain A1552

51.051

93.539

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.182

92.697

0.447
  pilU Pseudomonas stutzeri DSM 10701

43.695

95.787

0.419
  pilU Vibrio cholerae strain A1552

41.593

95.225

0.396
  pilU Acinetobacter baylyi ADP1

40.95

94.663

0.388

Multiple sequence alignment