Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HORE_RS03180 Genome accession   NC_011899
Coordinates   650679..651752 (+) Length   357 a.a.
NCBI ID   WP_041605819.1    Uniprot ID   -
Organism   Halothermothrix orenii H 168     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645679..656752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HORE_RS03155 (Hore_05930) - 645951..646223 (+) 273 WP_012635538.1 FeoA family protein -
  HORE_RS03160 (Hore_05940) - 646371..647066 (+) 696 WP_012635539.1 Crp/Fnr family transcriptional regulator -
  HORE_RS03165 (Hore_05950) - 647078..647959 (+) 882 WP_012635540.1 DMT family transporter -
  HORE_RS03170 (Hore_05960) - 647961..648830 (+) 870 WP_012635541.1 shikimate dehydrogenase -
  HORE_RS03175 (Hore_05970) gspE 648991..650667 (+) 1677 WP_012635542.1 type II secretion system ATPase GspE -
  HORE_RS03180 (Hore_05980) pilT 650679..651752 (+) 1074 WP_041605819.1 type IV pilus twitching motility protein PilT Machinery gene
  HORE_RS03185 (Hore_05990) gspF 651872..653077 (+) 1206 WP_012635544.1 type II secretion system inner membrane protein GspF -
  HORE_RS12340 (Hore_06000) - 653254..653685 (+) 432 WP_012635545.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HORE_RS03195 (Hore_06010) - 653764..654504 (+) 741 WP_012635546.1 A24 family peptidase -
  HORE_RS03200 (Hore_06020) - 654733..656022 (+) 1290 WP_012635547.1 hypothetical protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40411.54 Da        Isoelectric Point: 9.3547

>NTDB_id=32641 HORE_RS03180 WP_041605819.1 650679..651752(+) (pilT) [Halothermothrix orenii H 168]
MDIKEFLKKFVEDQHISDLHLTVNSKPVVRYDGKLKVFEEFSGRIGFEDMQNITKELMTDDQWQQFQEKGELDFSYSVPG
FSRFRVNAYYQRGAIALALRIIPTKIPTIEELGLPQILKNLALMRKGLFLCTGPTGSGKTTTLASMINIINKNRQAHILT
LEDPIEYLHRHNKSIVHQREVGIDTTSFKEGLRSALRQDPDVILVGEMRDHETISIALEAAETGHLVLATLHTNDAPQTI
DRIIDVFPPQQQRQVRVQLSLTLNGIVSQQLLPRKDGNGRIAALEVLIGTPAVRNVIRDGKSHQLYSSMQTGGKYGMITM
DNYILDLLSKSLINKDTALRRANDPEYIKKRINAVRV

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=32641 HORE_RS03180 WP_041605819.1 650679..651752(+) (pilT) [Halothermothrix orenii H 168]
ATGGATATCAAAGAATTTTTAAAAAAATTTGTAGAAGACCAACATATTTCTGATCTTCATCTTACAGTTAATTCTAAACC
TGTAGTCAGATATGACGGAAAACTCAAAGTATTTGAAGAATTTTCGGGTCGGATTGGGTTTGAGGATATGCAGAATATTA
CTAAAGAACTAATGACAGATGACCAGTGGCAACAATTTCAGGAAAAGGGTGAGCTTGATTTTTCCTATAGTGTTCCCGGT
TTTTCCCGGTTCCGGGTTAATGCTTATTACCAGAGGGGGGCTATTGCCCTGGCTTTGAGGATAATCCCGACAAAAATACC
AACCATCGAAGAGCTGGGTCTACCGCAAATTTTAAAAAACCTGGCCCTGATGAGAAAGGGACTGTTCCTGTGTACCGGCC
CTACTGGAAGCGGGAAGACAACAACCCTGGCTTCTATGATTAATATTATCAATAAAAATAGACAGGCCCACATCCTGACC
CTTGAAGACCCCATAGAATATTTACACCGCCATAATAAAAGTATTGTTCATCAAAGGGAAGTGGGGATTGATACTACAAG
TTTTAAAGAAGGGTTGAGATCTGCCCTGAGGCAGGACCCTGACGTTATTCTGGTTGGGGAGATGAGGGATCATGAAACAA
TATCCATTGCCTTAGAAGCAGCCGAGACCGGTCATCTTGTTCTGGCAACATTACATACGAATGATGCTCCCCAGACCATT
GATCGTATTATAGATGTTTTTCCTCCCCAACAGCAACGACAGGTCAGGGTGCAGTTATCACTTACTCTTAACGGGATTGT
TTCCCAGCAATTGCTGCCCCGGAAGGATGGTAATGGCAGGATAGCAGCCCTGGAGGTATTAATTGGAACCCCGGCTGTCA
GAAATGTCATCAGGGATGGAAAGAGTCATCAACTTTATTCCAGTATGCAGACCGGTGGGAAATACGGAATGATCACCATG
GACAACTATATACTTGATTTATTAAGTAAAAGTCTTATTAATAAGGATACTGCCCTGAGAAGGGCCAATGACCCGGAATA
TATCAAAAAGAGGATTAATGCGGTCAGGGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.704

99.44

0.504
  pilT Acinetobacter baylyi ADP1

50.142

98.319

0.493
  pilT Pseudomonas stutzeri DSM 10701

49.573

98.319

0.487
  pilT Pseudomonas aeruginosa PAK

48.718

98.319

0.479
  pilT Acinetobacter nosocomialis M2

49.422

96.919

0.479
  pilT Acinetobacter baumannii strain A118

49.133

96.919

0.476
  pilT Acinetobacter baumannii D1279779

49.133

96.919

0.476
  pilT Vibrio cholerae strain A1552

50

94.118

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.118

0.471
  pilT Legionella pneumophila strain Lp02

49.408

94.678

0.468
  pilT Legionella pneumophila strain ERS1305867

49.408

94.678

0.468
  pilT Neisseria meningitidis 8013

47.578

98.319

0.468
  pilT Neisseria gonorrhoeae MS11

47.293

98.319

0.465
  pilU Pseudomonas stutzeri DSM 10701

45.481

96.078

0.437
  pilU Acinetobacter baylyi ADP1

40.51

98.88

0.401
  pilU Vibrio cholerae strain A1552

41.108

96.078

0.395

Multiple sequence alignment