Detailed information NTG0032633

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: CCEL_RS12185
• Genome accession: NC_011898
• Coordinates: 2907017..2908072 (-)
• Length: 351 a.a.
• NCBI ID: WP_015925832.1
• Uniprot ID: B8I5K7
• Organism: Ruminiclostridium cellulolyticum H10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2902017..2913072

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CCEL_RS12160 (Ccel_2408)	-	2902796..2903320 (-)	525	WP_015925827.1	hypothetical protein	-
CCEL_RS12165 (Ccel_2409)	pilM	2903360..2904415 (-)	1056	WP_015925828.1	type IV pilus assembly protein PilM	-
CCEL_RS12170 (Ccel_2410)	-	2904446..2905243 (-)	798	WP_015925829.1	A24 family peptidase	-
CCEL_RS12175 (Ccel_2411)	-	2905248..2905634 (-)	387	WP_015925830.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CCEL_RS12180 (Ccel_2412)	-	2905673..2906890 (-)	1218	WP_015925831.1	type II secretion system F family protein	-
CCEL_RS12185 (Ccel_2413)	pilT	2907017..2908072 (-)	1056	WP_015925832.1	type IV pilus twitching motility protein PilT	Machinery gene
CCEL_RS12190 (Ccel_2414)	-	2908131..2909825 (-)	1695	WP_015925833.1	GspE/PulE family protein	-

Sequence

Protein

• Length: 351 a.a.
• Molecular weight: 38987.15 Da
• Isoelectric Point: 7.3991

>NTDB_id=32633 CCEL_RS12185 WP_015925832.1 2907017..2908072(-) (pilT) [Ruminiclostridium cellulolyticum H10]
MLSLEDLLKKTLEFGASDLHLTVGIPPTIRKNGRLSTIGEEKLMPSDTETFVRSMLNEEQWRKYQDTGELDLSFALKGMG
RFRVNVYKQRGTCCAAIRMVNLVIPSIEELGLPNIIMDMSRKTKGMILVTGPTGSGKSTTLALLIDLINKNRDCHILTLE
DPIEYLHKHNKSIVNQREIGNDSQSYSAALRAALREDPDVILVGEMRDTETIAIAVTAAETGHLVLSTLHTIGAANTIDR
IIDVFPPYQQQQIKVQLSTVIQSVISQQLLPRKDKLGRVPAIEIMVATPAVRNLIREGKTYQINSQIQTGAKFGMQAMDS
SLASLYKKGIISQEDAMTYAMDPDNIIRYMG

Nucleotide

• Length: 1056 bp

>NTDB_id=32633 CCEL_RS12185 WP_015925832.1 2907017..2908072(-) (pilT) [Ruminiclostridium cellulolyticum H10]
ATGCTTTCATTAGAGGATTTGCTAAAAAAAACTTTGGAATTTGGTGCTTCTGACTTACATTTAACAGTAGGGATTCCCCC
AACTATCAGAAAGAACGGCAGACTTTCTACCATAGGTGAGGAAAAACTCATGCCCTCGGATACTGAAACGTTTGTCAGAA
GTATGCTCAATGAAGAACAGTGGAGGAAATATCAGGATACAGGAGAACTTGATCTTTCATTTGCACTTAAAGGTATGGGA
AGATTCAGAGTTAATGTTTACAAGCAAAGAGGTACCTGCTGTGCTGCTATAAGAATGGTAAACCTTGTTATTCCTTCAAT
TGAGGAACTTGGATTGCCAAATATAATTATGGATATGAGCAGGAAAACCAAGGGAATGATTTTAGTTACCGGCCCTACCG
GAAGTGGAAAGTCAACGACTCTGGCTTTGCTTATTGATTTGATAAACAAAAATAGGGACTGCCATATACTTACTTTGGAG
GATCCCATAGAGTATCTTCACAAACACAATAAATCCATTGTTAATCAAAGAGAAATAGGAAATGATTCGCAATCCTATTC
TGCTGCACTTAGAGCGGCTTTAAGAGAAGACCCTGATGTTATTCTGGTGGGAGAGATGCGAGATACCGAAACTATAGCTA
TAGCTGTTACTGCTGCCGAAACAGGACACTTGGTTCTGTCTACTCTGCATACTATCGGAGCGGCTAATACAATTGACAGA
ATAATAGATGTTTTCCCCCCATACCAGCAGCAGCAGATAAAAGTTCAGCTTTCTACGGTTATTCAGTCTGTTATTTCGCA
GCAGCTTCTTCCGAGAAAAGATAAGCTCGGAAGAGTGCCCGCTATTGAAATTATGGTAGCTACTCCTGCGGTAAGAAATT
TAATCCGTGAGGGTAAAACATATCAGATTAATTCTCAGATTCAGACAGGTGCAAAATTCGGAATGCAGGCAATGGACTCG
AGTCTGGCAAGTTTGTATAAAAAAGGTATCATAAGTCAGGAAGATGCAATGACTTATGCCATGGACCCAGACAATATTAT
CAGATACATGGGGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (81-270)

3D structure

• AlphaFold DB: B8I5K7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	52.161	98.86	0.516
pilT	Acinetobacter baylyi ADP1	49.565	98.291	0.487
pilT	Acinetobacter nosocomialis M2	49.133	98.575	0.484
pilT	Acinetobacter baumannii D1279779	49.133	98.575	0.484
pilT	Acinetobacter baumannii strain A118	49.133	98.575	0.484
pilT	Neisseria meningitidis 8013	49.275	98.291	0.484
pilT	Neisseria gonorrhoeae MS11	48.986	98.291	0.481
pilT	Legionella pneumophila strain Lp02	48.974	97.151	0.476
pilT	Legionella pneumophila strain ERS1305867	48.974	97.151	0.476
pilT	Pseudomonas aeruginosa PAK	48.116	98.291	0.473
pilT	Pseudomonas stutzeri DSM 10701	47.246	98.291	0.464
pilT	Vibrio cholerae strain A1552	48.071	96.011	0.462
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.071	96.011	0.462
pilU	Pseudomonas stutzeri DSM 10701	39.828	99.43	0.396
pilU	Vibrio cholerae strain A1552	38.395	99.43	0.382
pilU	Acinetobacter baylyi ADP1	37.572	98.575	0.37