Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A2CP1_RS03275 Genome accession   NC_011891
Coordinates   731557..732657 (+) Length   366 a.a.
NCBI ID   WP_012632054.1    Uniprot ID   B8JCY6
Organism   Anaeromyxobacter dehalogenans 2CP-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726557..737657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2CP1_RS03250 (A2cp1_0652) - 726950..727798 (+) 849 WP_012632049.1 purine-nucleoside phosphorylase -
  A2CP1_RS03255 (A2cp1_0653) - 727846..728199 (+) 354 WP_012632050.1 PilZ domain-containing protein -
  A2CP1_RS03260 (A2cp1_0654) - 728204..728608 (+) 405 WP_012632051.1 cytidine deaminase -
  A2CP1_RS03265 (A2cp1_0655) ribF 728682..729653 (+) 972 WP_012632052.1 riboflavin biosynthesis protein RibF -
  A2CP1_RS03270 (A2cp1_0656) pilB 729837..731543 (+) 1707 WP_012632053.1 type IV-A pilus assembly ATPase PilB -
  A2CP1_RS03275 (A2cp1_0657) pilT 731557..732657 (+) 1101 WP_012632054.1 type IV pilus twitching motility protein PilT Machinery gene
  A2CP1_RS03280 (A2cp1_0658) - 732726..733979 (+) 1254 WP_012632055.1 type II secretion system F family protein -
  A2CP1_RS03285 (A2cp1_0659) - 733990..735567 (+) 1578 WP_012632056.1 ATP-binding protein -
  A2CP1_RS03290 (A2cp1_0660) - 735580..736965 (+) 1386 WP_012632057.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40106.50 Da        Isoelectric Point: 9.2896

>NTDB_id=32598 A2CP1_RS03275 WP_012632054.1 731557..732657(+) (pilT) [Anaeromyxobacter dehalogenans 2CP-1]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLS
RFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELAKKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIE
DPIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPRAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQAKFGMQTFN
QSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGPPQGAAR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=32598 A2CP1_RS03275 WP_012632054.1 731557..732657(+) (pilT) [Anaeromyxobacter dehalogenans 2CP-1]
TTGGCGAACCTGCACCAGCTCCTGAAGGCGATGGTCGAGAAGGGGGCCTCCGACCTCCACGTCACCACCGGCTCCCCGCC
CCAGCTCCGCATCGACGGCAAGCTGGTGCCGCTGAAGACCCCGCCGCTCTCGCCCGTCGAGACGAAGCAGCTCTGCTACT
CGATCCTCACCGACGCGCAGAAGCACAAGTTCGAGGAGGAGAGCGAGCTCGACCTCTCCTTCGGCGTGAAGGGGCTCTCG
CGCTTCCGCGCGAACGTCTTCATGCAGCGCGGCGCGGTGGCCGGCGCGTTCCGCACCATCCCGTTCAAGATCCTCACCTT
CCAGGAGCTGGGCCTGCCGCCCATCGTGGCCGAGCTGGCCAAGAAGCCGCGCGGCCTGGTGCTCGTCACCGGCCCCACCG
GCTCCGGCAAGTCCACCACGCTCGCGTCGATCATCGACAAGATCAACACGGACCGCCACGAGCACATCATCACGATCGAG
GATCCGATCGAGTACCTGCACCCGCACAAGAACTGCGTGGTGAACCAGCGCGAGGTCGGGGCGGACACCGCCTCGTTCAA
GAAGGCGCTGAAGTACATCCTCCGCCAGGATCCCGACGTCGTGCTCGTCGGCGAGATGCGCGATCTCGAGACCATCGAGG
CGGCGCTGGTGATCTCCGAGACGGGCCACCTCGCCTTCGCGACGCTGCACACCAACAGCGCGGTCCAGACGATCAACCGC
ATCCTGGACGTGTTCCCGCCCTACCAGCAGCCGCAGGTCCGCGCGCAGCTCTCCTTCGTGCTGGAGGGCGTGCTCACCCA
GAACCTGCTGCCCAGGGCCGGCGGCCCCGGCCGCGTGCTCATCATCGAGGTGATGGTCCCGAACCCCGCGATCCGGAACC
TCATCCGCGAGGACAAGGTGCACCAGGTCTACTCGCAGATGCAGGTCGGCCAGGCCAAGTTCGGCATGCAGACCTTCAAC
CAGAGCCTGGCGATGGCGTTCGCGAAGCGCCTCATCACGCTGGAGGAGGCGCTCGGCCGGTCCTCCGACGCGGAGGAGCT
GAAGAACATCATCGCGAGCGGCGCCGCGCTGCGCGGGCCGCCGCAAGGTGCGGCGCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8JCY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.26

96.721

0.505
  pilT Legionella pneumophila strain Lp02

52.77

93.716

0.495
  pilT Legionella pneumophila strain ERS1305867

52.77

93.716

0.495
  pilT Acinetobacter baylyi ADP1

52.239

91.53

0.478
  pilT Pseudomonas aeruginosa PAK

51.642

91.53

0.473
  pilT Acinetobacter baumannii D1279779

51.045

91.53

0.467
  pilT Acinetobacter baumannii strain A118

51.045

91.53

0.467
  pilT Acinetobacter nosocomialis M2

50.746

91.53

0.464
  pilT Pseudomonas stutzeri DSM 10701

50.746

91.53

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.701

91.257

0.454
  pilT Vibrio cholerae strain A1552

49.701

91.257

0.454
  pilT Neisseria gonorrhoeae MS11

46.921

93.169

0.437
  pilT Neisseria meningitidis 8013

46.921

93.169

0.437
  pilU Pseudomonas stutzeri DSM 10701

42.135

97.268

0.41
  pilU Vibrio cholerae strain A1552

41.16

98.907

0.407
  pilU Acinetobacter baylyi ADP1

39.481

94.809

0.374

Multiple sequence alignment