Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EFZ62_RS18370 Genome accession   NZ_CP033623
Coordinates   3859276..3860298 (-) Length   340 a.a.
NCBI ID   WP_060422659.1    Uniprot ID   -
Organism   Serratia marcescens strain N10A28     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3854276..3865298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFZ62_RS18340 (EFZ62_18415) - 3855077..3855589 (+) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -
  EFZ62_RS18345 (EFZ62_18420) endA 3855691..3856386 (+) 696 WP_038879275.1 deoxyribonuclease I -
  EFZ62_RS18350 (EFZ62_18425) rsmE 3856456..3857187 (+) 732 WP_060430995.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EFZ62_RS18355 (EFZ62_18430) gshB 3857198..3858148 (+) 951 WP_047729854.1 glutathione synthase -
  EFZ62_RS18360 (EFZ62_18435) - 3858294..3858857 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  EFZ62_RS18365 (EFZ62_18440) ruvX 3858857..3859279 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  EFZ62_RS18370 (EFZ62_18445) pilT 3859276..3860298 (-) 1023 WP_060422659.1 type IV pilus twitching motility protein PilT Machinery gene
  EFZ62_RS18375 (EFZ62_18450) - 3860319..3861026 (+) 708 WP_060422654.1 YggS family pyridoxal phosphate-dependent enzyme -
  EFZ62_RS18380 (EFZ62_18455) proC 3861046..3861867 (+) 822 WP_060422650.1 pyrroline-5-carboxylate reductase -
  EFZ62_RS18385 (EFZ62_18460) - 3861899..3862453 (+) 555 WP_019456092.1 YggT family protein -
  EFZ62_RS18390 (EFZ62_18465) yggU 3862450..3862740 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  EFZ62_RS18395 (EFZ62_18470) - 3862758..3863351 (+) 594 WP_060422645.1 XTP/dITP diphosphatase -
  EFZ62_RS18400 (EFZ62_18475) hemW 3863344..3864486 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  EFZ62_RS18405 (EFZ62_18480) - 3864524..3864958 (-) 435 WP_100397087.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36473.82 Da        Isoelectric Point: 7.2889

>NTDB_id=324541 EFZ62_RS18370 WP_060422659.1 3859276..3860298(-) (pilT) [Serratia marcescens strain N10A28]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQRAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDTALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=324541 EFZ62_RS18370 WP_060422659.1 3859276..3860298(-) (pilT) [Serratia marcescens strain N10A28]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAACGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCACTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATTGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGTCGGCGATCGCTGATTCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGTTGCGCGCTGCGTTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATTGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Neisseria meningitidis 8013

50

98.824

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

50

97.647

0.488
  pilT Pseudomonas aeruginosa PAK

49.697

97.059

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Pseudomonas stutzeri DSM 10701

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Vibrio cholerae strain A1552

40.184

95.882

0.385
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.198

95.294

0.374

Multiple sequence alignment