Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EEY24_RS09015 Genome accession   NZ_CP033575
Coordinates   1742950..1743987 (+) Length   345 a.a.
NCBI ID   WP_025009461.1    Uniprot ID   A0A1S2TIJ8
Organism   Shewanella algae strain KC-Na-R1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737950..1748987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEY24_RS08980 (EEY24_08975) hemW 1737950..1739086 (-) 1137 WP_123115951.1 radical SAM family heme chaperone HemW -
  EEY24_RS08985 (EEY24_08980) rdgB 1739086..1739688 (-) 603 WP_025009467.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  EEY24_RS08990 (EEY24_08985) - 1739908..1740342 (-) 435 WP_025887551.1 DUF4426 domain-containing protein -
  EEY24_RS08995 (EEY24_08990) yggU 1740405..1740692 (-) 288 WP_037467122.1 DUF167 family protein YggU -
  EEY24_RS09000 (EEY24_08995) - 1740692..1741243 (-) 552 WP_025887553.1 YggT family protein -
  EEY24_RS09005 (EEY24_09000) proC 1741267..1742085 (-) 819 WP_123115952.1 pyrroline-5-carboxylate reductase -
  EEY24_RS09010 (EEY24_09005) - 1742218..1742913 (-) 696 WP_054744099.1 YggS family pyridoxal phosphate-dependent enzyme -
  EEY24_RS09015 (EEY24_09010) pilT 1742950..1743987 (+) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  EEY24_RS09020 (EEY24_09015) pilU 1743997..1745109 (+) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EEY24_RS09025 (EEY24_09020) - 1745234..1746343 (+) 1110 WP_062793317.1 hypothetical protein -
  EEY24_RS09030 (EEY24_09025) - 1746383..1746964 (+) 582 WP_123115953.1 glutathione peroxidase -
  EEY24_RS09035 (EEY24_09030) hemH 1746999..1748012 (+) 1014 WP_025009459.1 ferrochelatase -
  EEY24_RS09040 (EEY24_09035) - 1748188..1748922 (+) 735 WP_071237900.1 tryptophan 2,3-dioxygenase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.97 Da        Isoelectric Point: 6.8739

>NTDB_id=324000 EEY24_RS09015 WP_025009461.1 1742950..1743987(+) (pilT) [Shewanella algae strain KC-Na-R1]
MEITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINVPALDHQGVHRLVYDIMNDKQRKDYEEFLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILTLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QNLVNRGLISREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=324000 EEY24_RS09015 WP_025009461.1 1742950..1743987(+) (pilT) [Shewanella algae strain KC-Na-R1]
ATGGAAATCACTGAATTATTGGCCTTTAGTGTAAAGCATAACGCTTCGGATCTGCACCTGTCGGCCGGTGTGCCTCCCAT
GATACGCGTCGACGGCGAGGTCAGAAAGATTAATGTCCCTGCGCTTGATCACCAAGGGGTTCACAGACTTGTCTACGACA
TAATGAATGACAAGCAGCGTAAGGATTATGAAGAGTTTCTGGAAATCGACTTTTCCTTTGAAGTCCCCAATCTGGCGCGT
TTTCGTGTCAATGCCTTCAATCAGTCCCGCGGCGCCGCCGCGGTTTTTCGTACTATTCCCAGCGACATTTTGACTTTGGA
ACAACTGGGCGCACCGGAAATCTTCAAGAAAATTGCTTCATATCCTCGCGGCCTGGTGTTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACCACCCTGGCGGCGATGATAGATTACGTCAATGAGAACCGCCACGATCATATTCTCACCATTGAAGAC
CCTATCGAATTCGTGCACCAAAACAAGCAATGTCTTATCAACCAGCGGGAAGTGCATCGCCATACCCACAGCTTTAACGC
CGCCCTCAGAAGCGCCCTGCGTGAAGACCCCGATGTCATCCTGGTCGGTGAGATGCGGGATTTGGAAACCATACGCCTGG
CGATGACCGCGGCAGAAACCGGCCATTTGGTGTTCGGTACCTTGCACACCACCTCGGCGGCCAAGACCATTGACCGTGTG
GTTGACGTATTCCCCGCAGGCGAGAAGGACATGGTGCGTACCATGTTGTCTGAGTCGTTGCAGGCGGTTATCTCCCAGAC
CCTGATCAAGAAAGTTGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGATGGGAACCCCGGCTATCCGAAACCTTATCC
GTGAGGACAAGGTGGCACAGATGTATTCTGCTATCCAAACCGGTATGGCCCACGGCATGCAAACCTTGGAGCAGAGCCTG
CAGAACCTGGTGAATCGCGGCTTGATCAGTCGGGAAGATGCCATGGCCAAGAGCTCCAACAAACAAGCGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2TIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.871

99.13

0.812
  pilT Acinetobacter baumannii D1279779

81.871

99.13

0.812
  pilT Acinetobacter baumannii strain A118

81.871

99.13

0.812
  pilT Acinetobacter baylyi ADP1

78.426

99.42

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment