Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EEA47_RS06700 Genome accession   NZ_CP033457
Coordinates   1416796..1417902 (-) Length   368 a.a.
NCBI ID   WP_213908005.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain SNU_SP1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1411796..1422902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEA47_RS06670 (EEA47_06920) - 1412428..1412925 (+) 498 WP_054866486.1 SprT family zinc-dependent metalloprotease -
  EEA47_RS06675 (EEA47_06925) endA 1413076..1413771 (+) 696 WP_053306786.1 deoxyribonuclease I -
  EEA47_RS06680 (EEA47_06930) rsmE 1413885..1414616 (+) 732 WP_053304141.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EEA47_RS06685 (EEA47_06935) gshB 1414631..1415581 (+) 951 WP_005381021.1 glutathione synthase -
  EEA47_RS06690 (EEA47_06940) - 1415710..1416273 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  EEA47_RS06695 (EEA47_06945) ruvX 1416309..1416734 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  EEA47_RS06700 (EEA47_06950) pilU 1416796..1417902 (-) 1107 WP_213908005.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EEA47_RS06705 (EEA47_06955) pilT 1417933..1418973 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  EEA47_RS06710 (EEA47_06960) - 1419002..1419703 (+) 702 WP_005384521.1 YggS family pyridoxal phosphate-dependent enzyme -
  EEA47_RS06715 (EEA47_06965) proC 1419863..1420681 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  EEA47_RS06720 (EEA47_06970) - 1420734..1421291 (+) 558 WP_005381012.1 YggT family protein -
  EEA47_RS06725 (EEA47_06975) yggU 1421291..1421581 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  EEA47_RS06730 (EEA47_06980) - 1421711..1422142 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  EEA47_RS06735 (EEA47_06985) - 1422261..1422863 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41268.39 Da        Isoelectric Point: 6.5943

>NTDB_id=323800 EEA47_RS06700 WP_213908005.1 1416796..1417902(-) (pilU) [Vibrio alginolyticus strain SNU_SP1]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREIGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=323800 EEA47_RS06700 WP_213908005.1 1416796..1417902(-) (pilU) [Vibrio alginolyticus strain SNU_SP1]
ATGGATCTAGATAAATTTCTCGAAGGCATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTCACCGAGAGTGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATCCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAATTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCCTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCAAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.923

88.315

0.397
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.39

89.946

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment