Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EFER_RS14535 Genome accession   NC_011740
Coordinates   2955536..2956516 (-) Length   326 a.a.
NCBI ID   WP_015953696.1    Uniprot ID   B7LPR7
Organism   Escherichia fergusonii ATCC 35469     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2950536..2961516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFER_RS14505 (EFER_2883) - 2951368..2951865 (+) 498 WP_015953694.1 SprT family zinc-dependent metalloprotease -
  EFER_RS14510 (EFER_2884) endA 2951960..2952667 (+) 708 WP_000286513.1 deoxyribonuclease I -
  EFER_RS14515 (EFER_2885) rsmE 2952747..2953478 (+) 732 WP_015953695.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EFER_RS14520 (EFER_2886) gshB 2953491..2954441 (+) 951 WP_000593298.1 glutathione synthase -
  EFER_RS14525 (EFER_2887) - 2954550..2955113 (+) 564 WP_002431379.1 YqgE/AlgH family protein -
  EFER_RS14530 (EFER_2888) ruvX 2955113..2955529 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  EFER_RS14535 (EFER_2889) pilT 2955536..2956516 (-) 981 WP_015953696.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EFER_RS14540 (EFER_2890) yggS 2956534..2957238 (+) 705 WP_000997798.1 pyridoxal phosphate homeostasis protein -
  EFER_RS14545 (EFER_2891) yggT 2957256..2957822 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  EFER_RS14550 (EFER_2892) yggU 2957819..2958109 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  EFER_RS14555 (EFER_2893) - 2958117..2958710 (+) 594 WP_001174755.1 XTP/dITP diphosphatase -
  EFER_RS14560 (EFER_2894) hemW 2958703..2959839 (+) 1137 WP_000239968.1 radical SAM family heme chaperone HemW -
  EFER_RS14565 (EFER_2895) - 2960153..2961139 (+) 987 WP_000783994.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36001.23 Da        Isoelectric Point: 5.6101

>NTDB_id=32166 EFER_RS14535 WP_015953696.1 2955536..2956516(-) (pilT) [Escherichia fergusonii ATCC 35469]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSYCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YFYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQLR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=32166 EFER_RS14535 WP_015953696.1 2955536..2956516(-) (pilT) [Escherichia fergusonii ATCC 35469]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTGGAAGAGCTATTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAACGGTCAGTTAGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGTACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGAAAAT
CTACCACGCTGGCAGCGATGGTTGGTTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATTTCTATGCCAGCCAGCGGTGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTGGCGACCTTACATACACGCGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGAGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCTGCGG
GTGGGGGAAGGACGTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LPR7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

41.27

96.626

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.344

100

0.383
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368

Multiple sequence alignment