Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D9842_RS13440 Genome accession   NZ_CP033043
Coordinates   2684355..2685395 (-) Length   346 a.a.
NCBI ID   WP_121662982.1    Uniprot ID   -
Organism   Metabacillus litoralis strain Bac94     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2679355..2690395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9842_RS13410 - 2679372..2680151 (-) 780 WP_121662976.1 pilus assembly protein PilO -
  D9842_RS13415 - 2680148..2680732 (-) 585 WP_121662977.1 PilN domain-containing protein -
  D9842_RS13420 pilM 2680739..2681734 (-) 996 WP_121662978.1 type IV pilus biogenesis protein PilM -
  D9842_RS13425 - 2681751..2682524 (-) 774 WP_121662979.1 A24 family peptidase -
  D9842_RS13430 - 2682592..2683053 (-) 462 WP_121662980.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D9842_RS13435 - 2683147..2684355 (-) 1209 WP_121662981.1 type II secretion system F family protein -
  D9842_RS13440 pilT 2684355..2685395 (-) 1041 WP_121662982.1 type IV pilus twitching motility protein PilT Machinery gene
  D9842_RS13445 - 2685405..2687066 (-) 1662 WP_121662983.1 GspE/PulE family protein -
  D9842_RS13450 - 2687091..2688407 (-) 1317 WP_121662984.1 VanW family protein -
  D9842_RS13455 - 2688424..2689215 (-) 792 WP_121662985.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37817.61 Da        Isoelectric Point: 7.9662

>NTDB_id=320614 D9842_RS13440 WP_121662982.1 2684355..2685395(-) (pilT) [Metabacillus litoralis strain Bac94]
MKAKIDSILKLAYEKQASDIHLSVGVPPVIRINGELVRIGENMLMPADTEEMAKGMISSAKWSLFEEKGELDFSYGIPGV
SRFRVNAFRQRSCISLAVRIVPRNIPSIDDLQLPSILKSIVEKPHGLILVTGPTGSGKSTTLASMISFMNQTMKRHVITL
EDPIEYLHKHQKCIVDQREVGFDTGSFASGLKAALRQDPDVILVGELRDLETIHTAITAAETGHLVLGTLHTSSAASTVD
RIIDVFPANQQPQIRLQLASVLVGVISQRLFPTVDKSGRRAVTEILLNNSAVASLIRNEKTYQIPSVIQTSRAQGMQTFE
SSLSEYVKAGIISSEAALPYKQGTTD

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=320614 D9842_RS13440 WP_121662982.1 2684355..2685395(-) (pilT) [Metabacillus litoralis strain Bac94]
TTGAAAGCAAAAATTGACTCTATATTAAAGCTTGCTTATGAAAAGCAAGCTTCAGACATTCATTTGTCAGTTGGTGTGCC
TCCCGTTATACGAATTAACGGCGAGTTAGTTCGAATTGGCGAAAACATGTTAATGCCTGCTGATACAGAAGAAATGGCAA
AGGGAATGATTTCTTCAGCAAAATGGTCATTGTTTGAAGAAAAAGGAGAGCTTGATTTTTCTTACGGAATTCCAGGTGTA
TCTCGCTTTCGTGTTAATGCATTTCGTCAGCGCTCTTGTATCTCTCTAGCTGTACGAATTGTTCCAAGAAATATTCCATC
AATTGACGATTTGCAGTTGCCATCGATTTTAAAATCAATTGTGGAAAAGCCCCATGGACTTATCTTAGTAACTGGACCAA
CCGGCAGTGGAAAATCAACAACACTTGCGTCGATGATTTCCTTTATGAATCAAACGATGAAGCGCCATGTTATTACACTT
GAGGATCCGATTGAATATTTGCATAAACATCAAAAATGTATTGTTGATCAACGTGAAGTTGGTTTTGATACAGGTAGCTT
TGCATCAGGTTTAAAAGCAGCACTAAGACAGGATCCAGATGTGATTTTAGTTGGAGAGCTTCGCGATTTAGAAACAATTC
ACACAGCAATAACTGCAGCAGAAACAGGACATCTTGTTCTTGGTACACTTCACACGTCGTCTGCTGCATCAACAGTTGAT
AGGATCATCGATGTGTTTCCGGCTAATCAGCAGCCACAAATCCGACTTCAGCTTGCTTCCGTGTTAGTAGGGGTTATATC
ACAAAGGCTTTTCCCGACAGTTGATAAATCAGGGCGCCGTGCTGTAACTGAAATTTTATTAAATAACTCGGCAGTTGCCA
GCTTAATACGAAATGAAAAAACATACCAAATTCCGAGCGTGATTCAAACAAGTCGAGCACAAGGAATGCAAACCTTTGAA
AGCTCTCTTTCAGAGTATGTAAAAGCAGGCATTATTTCTTCTGAAGCAGCTTTACCTTACAAACAAGGGACGACAGACTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.898

96.532

0.491
  pilT Neisseria gonorrhoeae MS11

48.503

96.532

0.468
  pilT Neisseria meningitidis 8013

48.204

96.532

0.465
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.64

95.665

0.465
  pilT Vibrio cholerae strain A1552

48.64

95.665

0.465
  pilT Acinetobacter nosocomialis M2

47.447

96.243

0.457
  pilT Legionella pneumophila strain ERS1305867

47.447

96.243

0.457
  pilT Legionella pneumophila strain Lp02

47.447

96.243

0.457
  pilT Acinetobacter baumannii D1279779

47.447

96.243

0.457
  pilT Acinetobacter baumannii strain A118

47.447

96.243

0.457
  pilT Pseudomonas stutzeri DSM 10701

46.847

96.243

0.451
  pilT Pseudomonas aeruginosa PAK

46.847

96.243

0.451
  pilT Acinetobacter baylyi ADP1

46.201

95.087

0.439
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.133

0.419
  pilU Acinetobacter baylyi ADP1

41.543

97.399

0.405
  pilU Vibrio cholerae strain A1552

40.785

95.665

0.39

Multiple sequence alignment