Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DTUR_RS04445 Genome accession   NC_011661
Coordinates   902352..903434 (+) Length   360 a.a.
NCBI ID   WP_012583239.1    Uniprot ID   B8E072
Organism   Dictyoglomus turgidum DSM 6724     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 897352..908434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTUR_RS04425 (Dtur_0870) - 897840..898175 (+) 336 WP_012583235.1 DUF1292 domain-containing protein -
  DTUR_RS04430 (Dtur_0871) mltG 898093..899187 (+) 1095 WP_242603758.1 endolytic transglycosylase MltG -
  DTUR_RS04435 (Dtur_0872) - 899184..899717 (+) 534 WP_242603760.1 YqeG family HAD IIIA-type phosphatase -
  DTUR_RS04440 (Dtur_0873) gspE 899719..902334 (+) 2616 WP_012583238.1 type II secretion system ATPase GspE -
  DTUR_RS04445 (Dtur_0874) pilT 902352..903434 (+) 1083 WP_012583239.1 type IV pilus twitching motility protein PilT Machinery gene
  DTUR_RS04450 (Dtur_0875) - 903443..904672 (+) 1230 WP_012583240.1 type II secretion system F family protein -
  DTUR_RS04455 (Dtur_0876) - 904696..905376 (+) 681 WP_242603762.1 sigma-70 family RNA polymerase sigma factor -
  DTUR_RS04460 (Dtur_0877) - 905472..905900 (+) 429 WP_012583242.1 type II secretion system protein -
  DTUR_RS04465 (Dtur_0878) - 906062..906490 (+) 429 WP_012583243.1 type II secretion system protein -
  DTUR_RS04470 (Dtur_0879) - 906583..907008 (+) 426 WP_012583244.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DTUR_RS04475 (Dtur_0880) - 907014..907436 (+) 423 WP_012583245.1 DUF47 family protein -
  DTUR_RS04480 (Dtur_0881) radC 907417..908103 (+) 687 WP_012583246.1 DNA repair protein RadC -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40177.38 Da        Isoelectric Point: 7.6060

>NTDB_id=31995 DTUR_RS04445 WP_012583239.1 902352..903434(+) (pilT) [Dictyoglomus turgidum DSM 6724]
MRDDLSIIEILQNALQKEASDVHITSGIPPVFRIKGHLIPQTQYPVLTPQLTYRLVFSLLTEEQKQKLQENKELDFSVGI
SGEGRFRVNAFWQRGSLAAVFRLIPWKIPTIEELRLPLVLKDLAALPRGLILVTGPTGSGKSTTLASMIDYINSNFSRHI
ITIEDPIEYLHTHKKSIVNQREVGSDTYSFANALRSALREDPDVILVGEMRDLETTAIAITAAETGHLVMATLHTVDAVQ
SIERIVDQFPAHQQQQIRLQLSGVIQGIISQQLLPRRDGTGRIVATEILIGIPAVRSLIREGKTPQIYSVIQTGGRYGMQ
TMDQSLADLVKKGLISQEVAFERAVNREELIRLLGGEVRR

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=31995 DTUR_RS04445 WP_012583239.1 902352..903434(+) (pilT) [Dictyoglomus turgidum DSM 6724]
ATGAGAGATGATCTTTCCATAATTGAAATTCTTCAAAACGCTCTTCAAAAAGAGGCTTCCGACGTACATATAACTTCTGG
AATTCCTCCAGTTTTCCGTATAAAAGGACATTTAATACCTCAAACCCAATATCCTGTTTTAACTCCTCAGCTAACTTATA
GACTTGTCTTTAGCCTTTTGACCGAAGAGCAAAAACAAAAATTACAAGAAAATAAGGAATTAGATTTTTCAGTGGGAATT
AGTGGGGAGGGAAGATTTAGAGTAAATGCTTTTTGGCAAAGAGGAAGTCTTGCTGCTGTATTTAGATTGATACCTTGGAA
AATTCCAACTATAGAAGAGCTGAGACTTCCTCTTGTTTTAAAAGATTTAGCTGCTCTCCCAAGGGGATTAATCCTTGTTA
CTGGCCCTACAGGAAGTGGTAAGTCCACAACCCTCGCTTCTATGATTGATTATATAAACTCAAACTTCAGCAGACATATT
ATTACCATTGAAGATCCTATTGAATACTTGCATACCCATAAAAAATCTATTGTCAATCAGAGAGAAGTAGGAAGTGATAC
TTATTCCTTTGCGAATGCTTTGAGATCTGCATTAAGAGAGGATCCTGATGTGATTCTTGTGGGTGAGATGAGAGATTTAG
AAACTACAGCTATAGCCATAACTGCAGCAGAGACAGGACATTTGGTTATGGCAACTCTACACACTGTGGATGCGGTCCAA
AGTATAGAAAGAATAGTAGACCAATTTCCTGCCCATCAACAGCAACAAATAAGGCTTCAACTTTCAGGAGTCATACAAGG
TATTATATCTCAACAGCTTTTACCAAGAAGAGATGGTACAGGAAGAATTGTTGCCACAGAGATTTTAATTGGAATTCCAG
CGGTAAGGAGCCTCATAAGAGAAGGCAAGACTCCCCAAATATATAGTGTGATTCAAACGGGTGGAAGATATGGAATGCAG
ACTATGGATCAAAGTCTTGCAGATCTTGTGAAAAAGGGACTTATCTCTCAAGAAGTAGCCTTTGAAAGAGCGGTAAATAG
GGAAGAATTGATACGTCTTTTGGGGGGAGAAGTGAGGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8E072

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.091

98.056

0.55
  pilT Neisseria meningitidis 8013

52.89

96.111

0.508
  pilT Neisseria gonorrhoeae MS11

52.312

96.111

0.503
  pilT Pseudomonas aeruginosa PAK

52.941

94.444

0.5
  pilT Pseudomonas stutzeri DSM 10701

51.445

96.111

0.494
  pilT Acinetobacter baylyi ADP1

51.603

95.278

0.492
  pilT Acinetobacter baumannii D1279779

52.381

93.333

0.489
  pilT Acinetobacter nosocomialis M2

52.381

93.333

0.489
  pilT Acinetobacter baumannii strain A118

52.381

93.333

0.489
  pilT Legionella pneumophila strain Lp02

51.02

95.278

0.486
  pilT Legionella pneumophila strain ERS1305867

51.02

95.278

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.333

0.467
  pilT Vibrio cholerae strain A1552

50

93.333

0.467
  pilU Pseudomonas stutzeri DSM 10701

45.322

95

0.431
  pilU Vibrio cholerae strain A1552

43.064

96.111

0.414
  pilU Acinetobacter baylyi ADP1

43.917

93.611

0.411

Multiple sequence alignment