Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D7D81_RS14410 Genome accession   NZ_CP032760
Coordinates   2987130..2988200 (-) Length   356 a.a.
NCBI ID   WP_125992226.1    Uniprot ID   -
Organism   Halocella sp. SP3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982130..2993200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7D81_RS14380 (D7D81_14430) - 2983087..2983626 (-) 540 WP_164522271.1 type II secretion system protein -
  D7D81_RS14385 (D7D81_14435) - 2983623..2984042 (-) 420 WP_125990755.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D7D81_RS14390 (D7D81_14440) - 2984059..2984478 (-) 420 WP_125990756.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D7D81_RS14395 (D7D81_14445) - 2984502..2985251 (-) 750 WP_286169751.1 prepilin peptidase -
  D7D81_RS14400 (D7D81_14450) - 2985359..2985754 (-) 396 WP_125990757.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D7D81_RS14405 (D7D81_14455) - 2985893..2987104 (-) 1212 WP_125990758.1 type II secretion system F family protein -
  D7D81_RS14410 (D7D81_14460) pilT 2987130..2988200 (-) 1071 WP_125992226.1 type IV pilus twitching motility protein PilT Machinery gene
  D7D81_RS14415 (D7D81_14465) gspE 2988223..2989899 (-) 1677 WP_125990759.1 type II secretion system ATPase GspE -
  D7D81_RS14420 (D7D81_14470) - 2990058..2990921 (-) 864 WP_125990760.1 shikimate dehydrogenase -
  D7D81_RS14425 (D7D81_14475) - 2990914..2991804 (-) 891 WP_125990761.1 DMT family transporter -
  D7D81_RS14430 (D7D81_14480) - 2991820..2992512 (-) 693 WP_125990762.1 Crp/Fnr family transcriptional regulator -
  D7D81_RS14435 (D7D81_14485) - 2992522..2992977 (-) 456 WP_125992228.1 transcriptional repressor -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39669.66 Da        Isoelectric Point: 6.4163

>NTDB_id=318584 D7D81_RS14410 WP_125992226.1 2987130..2988200(-) (pilT) [Halocella sp. SP3-1]
MDIIGLLKSVVEDANISDLHLTVNSQAVVRTTGVLRAYEQYSHKFTFADLIEICRYLMDEDQWKAFQEQGELDFSYSVPG
FSRFRVNAYRQRGAVSLALRIIPTEIPTIDDLGLPEILKKMAVQRRGLVLCTGPTGSGKSTTLASMINEINQERKCHILT
LEDPIEYLHQHNNSIVHQREVGIDTRSFAAGLRAALRQDPDVILVGEMRDLETISIALESAETGHLVLATLHTTDAPKTV
DRIIDVFPAHQQQQVRIQLSSVLNGIIAQQLLPRADMEGMVAGLEVLIGTPAVRNIIREGKSSQLESIMQTGAKYGMIVM
NNYLISLYEKGLISMETAVRRSTNPEYIKKGITRLG

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=318584 D7D81_RS14410 WP_125992226.1 2987130..2988200(-) (pilT) [Halocella sp. SP3-1]
CTGGATATAATAGGATTATTAAAGAGTGTTGTTGAAGATGCTAATATCTCTGATCTCCATTTAACGGTAAATTCCCAGGC
TGTTGTCAGGACTACCGGGGTATTAAGGGCTTATGAACAATATTCTCATAAATTTACTTTTGCTGACCTGATTGAGATAT
GTCGGTATTTGATGGATGAGGATCAGTGGAAGGCCTTTCAGGAACAAGGGGAACTTGATTTTTCTTATAGTGTTCCCGGT
TTTTCCCGTTTCAGGGTAAATGCCTATCGCCAGAGGGGGGCTGTCAGTCTGGCCCTGCGAATTATTCCGACAGAGATACC
GACAATTGATGACCTGGGGCTGCCGGAAATACTCAAAAAAATGGCGGTTCAGCGCAGAGGCTTGGTCTTATGTACTGGGC
CAACCGGTAGTGGTAAATCAACTACCCTGGCCTCAATGATCAATGAGATTAATCAAGAGAGGAAATGTCATATCCTGACC
CTGGAGGACCCGATTGAATACCTCCATCAACATAATAATTCAATTGTCCATCAAAGGGAGGTAGGGATTGATACCAGATC
ATTTGCTGCAGGACTGCGGGCTGCCCTGCGTCAGGACCCGGATGTAATATTGGTAGGTGAGATGCGTGACCTGGAGACTA
TTTCTATTGCTCTGGAATCTGCTGAGACCGGTCATCTGGTGCTGGCTACTCTCCATACAACTGATGCCCCCAAGACTGTT
GATAGAATTATTGATGTTTTTCCAGCTCATCAACAACAGCAGGTTCGAATCCAGTTATCTTCTGTACTTAACGGGATTAT
TGCTCAACAGCTTTTGCCCCGGGCAGATATGGAGGGAATGGTGGCCGGGCTGGAGGTGTTAATTGGTACTCCAGCTGTAC
GCAATATTATTCGCGAGGGTAAAAGTTCACAACTGGAATCAATTATGCAGACAGGTGCCAAATACGGTATGATAGTGATG
AATAATTACCTGATATCACTTTACGAAAAGGGATTGATCAGTATGGAAACTGCAGTCAGGCGTTCTACTAATCCTGAATA
TATCAAAAAGGGCATCACCAGGCTTGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

98.315

0.486
  pilT Legionella pneumophila strain Lp02

49.133

97.191

0.478
  pilT Legionella pneumophila strain ERS1305867

49.133

97.191

0.478
  pilT Pseudomonas stutzeri DSM 10701

48.276

97.753

0.472
  pilT Pseudomonas aeruginosa PAK

47.989

97.753

0.469
  pilT Acinetobacter nosocomialis M2

47.989

97.753

0.469
  pilT Acinetobacter baumannii D1279779

47.989

97.753

0.469
  pilT Acinetobacter baumannii strain A118

47.989

97.753

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.702

94.382

0.469
  pilT Vibrio cholerae strain A1552

49.702

94.382

0.469
  pilT Acinetobacter baylyi ADP1

47.11

97.191

0.458
  pilT Neisseria meningitidis 8013

49.096

93.258

0.458
  pilT Neisseria gonorrhoeae MS11

48.795

93.258

0.455
  pilU Pseudomonas stutzeri DSM 10701

44.838

95.225

0.427
  pilU Acinetobacter baylyi ADP1

39.429

98.315

0.388
  pilU Vibrio cholerae strain A1552

41.486

90.73

0.376

Multiple sequence alignment