Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VSAL_RS03100 Genome accession   NC_011312
Coordinates   598773..599879 (+) Length   368 a.a.
NCBI ID   WP_012549354.1    Uniprot ID   A0A839I3J9
Organism   Aliivibrio salmonicida LFI1238     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 593773..604879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSAL_RS03065 (VSAL_I0538) - 594117..594713 (-) 597 WP_012549347.1 XTP/dITP diphosphatase -
  VSAL_RS03070 (VSAL_I0539) - 594724..595155 (-) 432 WP_012549348.1 DUF4426 domain-containing protein -
  VSAL_RS03075 (VSAL_I0540) yggU 595184..595478 (-) 295 Protein_550 DUF167 family protein YggU -
  VSAL_RS03080 (VSAL_I0541) - 595507..596061 (-) 555 WP_012549350.1 YggT family protein -
  VSAL_RS03085 (VSAL_I0542) proC 596137..596955 (-) 819 WP_012549351.1 pyrroline-5-carboxylate reductase -
  VSAL_RS03090 (VSAL_I0543) - 596942..597709 (-) 768 WP_012549352.1 YggS family pyridoxal phosphate-dependent enzyme -
  VSAL_RS03095 (VSAL_I0544) pilT 597733..598770 (+) 1038 WP_012549353.1 type IV pilus twitching motility protein PilT Machinery gene
  VSAL_RS03100 (VSAL_I0545) pilU 598773..599879 (+) 1107 WP_012549354.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VSAL_RS03105 (VSAL_I0546) ruvX 599983..600408 (-) 426 WP_012549355.1 Holliday junction resolvase RuvX -
  VSAL_RS03110 (VSAL_I0547) - 600405..600968 (-) 564 WP_012549356.1 YqgE/AlgH family protein -
  VSAL_RS03115 (VSAL_I0548) gshB 601042..601998 (-) 957 WP_023604091.1 glutathione synthase -
  VSAL_RS03120 (VSAL_I0549) rsmE 602012..602743 (-) 732 WP_012549358.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VSAL_RS03125 (VSAL_I0550) - 602822..603526 (-) 705 WP_044583181.1 endonuclease -
  VSAL_RS03130 (VSAL_I0551) - 603626..604111 (-) 486 WP_012549360.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41027.09 Da        Isoelectric Point: 6.3470

>NTDB_id=31642 VSAL_RS03100 WP_012549354.1 598773..599879(+) (pilU) [Aliivibrio salmonicida LFI1238]
MNLDDLLNQMVKQKASDIYITVDAPCLLRVDGVLQATGAKQTVESVTALLAEMMNDDREKEFTQTLEANFAIVKESGRFR
VSAFWQREQPGAVIRRIETKIPSMDSLYLPSIMKDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNDHKSGHILTVEDPI
EFVHPHNKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRTRETMKYAMMFAETGHLCMATLHANNANQALERILH
LVPKENKEQFLFDLSMNLKGVIAQQLVPDAQGNGRHGVFEVLLNTPRIADLIRRGDLHELKGTMTRSRESGMQTFDQCLF
DLVIDGKITETEALHSADSANDLRLMLKTKRGDSFTTSKFDNVSISLD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=31642 VSAL_RS03100 WP_012549354.1 598773..599879(+) (pilU) [Aliivibrio salmonicida LFI1238]
ATGAATTTGGATGATTTATTGAATCAGATGGTGAAGCAGAAAGCCTCTGATATTTATATTACGGTGGATGCACCGTGTTT
ATTACGTGTTGATGGCGTTTTACAAGCGACAGGGGCAAAGCAAACGGTAGAGTCTGTAACCGCCCTGTTGGCTGAGATGA
TGAATGATGATCGAGAAAAAGAATTTACTCAAACATTAGAAGCCAATTTTGCCATCGTAAAAGAGAGTGGTCGTTTTCGT
GTGAGTGCATTTTGGCAGCGTGAACAACCGGGGGCCGTGATCCGTAGAATTGAAACTAAAATCCCATCCATGGATTCATT
GTATTTACCGAGTATCATGAAAGACTTGGCGGTAGCTAAACGAGGATTGGTTCTAGTTGTGGGAGCGACGGGATCAGGAA
AATCGACCACAATGGCTGCGATGACTGGGTACCGGAATGATCATAAATCGGGTCATATTTTGACGGTGGAAGATCCGATT
GAATTTGTTCATCCACATAATAAATGCATCGTGACTCAACGTGAAGTTGGGCTAGATACAGACAGTTATGAAGTTGCTTT
AAAAAATTCATTACGCCAAGCGCCCGATATGATTTTGATTGGTGAAATTCGAACCAGAGAAACGATGAAGTACGCGATGA
TGTTTGCTGAGACTGGGCATTTGTGTATGGCAACGTTACACGCAAACAATGCAAACCAAGCATTAGAGCGTATTTTACAT
TTGGTTCCAAAAGAGAATAAAGAGCAATTTTTATTTGATCTCTCAATGAATTTAAAAGGTGTTATTGCTCAACAATTGGT
TCCTGATGCTCAGGGAAATGGGCGTCACGGTGTGTTTGAAGTCTTACTCAATACTCCCCGAATCGCCGATTTAATTCGTA
GAGGTGATCTGCATGAATTAAAAGGGACAATGACACGCTCTCGTGAATCAGGAATGCAGACGTTTGATCAATGCTTATTT
GATTTAGTAATTGATGGCAAGATTACAGAAACAGAAGCGCTGCATAGTGCGGATTCTGCAAATGACTTAAGGCTGATGCT
GAAGACTAAGCGTGGGGATTCATTTACCACATCAAAATTTGATAATGTGTCGATATCGTTAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839I3J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

75

100

0.75
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

92.12

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.691

93.207

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Vibrio cholerae strain A1552

41.916

90.761

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.916

90.761

0.38
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Neisseria meningitidis 8013

39.42

93.75

0.37
  pilT Neisseria gonorrhoeae MS11

39.42

93.75

0.37
  pilT Acinetobacter baylyi ADP1

40.606

89.674

0.364

Multiple sequence alignment