Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DICTH_RS03450 Genome accession   NC_011297
Coordinates   731433..732515 (+) Length   360 a.a.
NCBI ID   WP_012547666.1    Uniprot ID   A0A7C3KPN7
Organism   Dictyoglomus thermophilum H-6-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726433..737515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DICTH_RS03430 (DICTH_0718) - 726944..727249 (+) 306 WP_012547627.1 DUF1292 domain-containing protein -
  DICTH_RS03435 (DICTH_0719) mltG 727176..728261 (+) 1086 WP_236608281.1 endolytic transglycosylase MltG -
  DICTH_RS03440 (DICTH_0720) - 728270..728791 (+) 522 WP_012547241.1 YqeG family HAD IIIA-type phosphatase -
  DICTH_RS03445 (DICTH_0721) gspE 728793..731414 (+) 2622 WP_012548064.1 type II secretion system ATPase GspE -
  DICTH_RS03450 (DICTH_0722) pilT 731433..732515 (+) 1083 WP_012547666.1 type IV pilus twitching motility protein PilT Machinery gene
  DICTH_RS03455 (DICTH_0723) - 732524..733753 (+) 1230 WP_012548647.1 type II secretion system F family protein -
  DICTH_RS03460 (DICTH_0724) - 733750..734457 (+) 708 WP_012547556.1 sigma-70 family RNA polymerase sigma factor -
  DICTH_RS10345 (DICTH_0725) - 734551..735006 (+) 456 WP_012548544.1 type IV pilin protein -
  DICTH_RS03470 (DICTH_0726) - 735098..735520 (+) 423 WP_012547898.1 type IV pilin protein -
  DICTH_RS03475 (DICTH_0727) - 735541..735963 (+) 423 WP_012547792.1 DUF47 family protein -
  DICTH_RS03480 (DICTH_0728) radC 735944..736630 (+) 687 WP_012548066.1 DNA repair protein RadC -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40138.38 Da        Isoelectric Point: 7.6055

>NTDB_id=31629 DICTH_RS03450 WP_012547666.1 731433..732515(+) (pilT) [Dictyoglomus thermophilum H-6-12]
MKEELSIIEILQSALQKEASDVHITSGIPPVFRIKGHLIPQTQYPTLTPQLTYRLIFSLLTEEQKQKLQENKELDFSVGI
SGEGRFRVNAFWQRGSLAAVFRLIPWKIPTIEELRLPLVLKDLAALPRGLILVTGPTGSGKSTTLASMIDYINSNFSRHI
ITIEDPIEYLHTHKKSIVNQREVGSDTYSFANALRSALREDPDVILVGEMRDLETTAIAITAAETGHLVMATLHTVDAVQ
SIERIVDQFPAHQQQQIRLQLSGVIQGIISQQLLPRKDGTGRIVATEILIGIPAVRSLIREGKTPQIYSVIQTGGRYGMQ
TMDQSLADLVKKGLISQEVAFERAVNREELIRLLGGEVRR

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=31629 DICTH_RS03450 WP_012547666.1 731433..732515(+) (pilT) [Dictyoglomus thermophilum H-6-12]
ATGAAAGAAGAACTTTCGATTATTGAGATTCTCCAAAGTGCTCTTCAAAAGGAAGCCTCAGATGTGCATATTACATCAGG
AATTCCCCCAGTTTTTCGTATAAAGGGACATTTAATACCTCAAACCCAATATCCTACACTTACGCCTCAATTAACTTATA
GACTTATATTTAGCCTTTTGACTGAAGAGCAAAAGCAAAAATTACAGGAAAATAAAGAGTTGGATTTCTCAGTAGGAATC
AGCGGAGAGGGTAGGTTTAGAGTAAATGCTTTTTGGCAAAGAGGAAGCCTTGCAGCAGTATTTAGATTAATACCTTGGAA
AATTCCAACAATAGAGGAGTTAAGGTTACCATTAGTTTTGAAAGATTTGGCTGCTCTTCCAAGAGGACTAATACTAGTAA
CTGGACCTACAGGAAGTGGTAAGTCTACTACTCTTGCTTCTATGATTGACTATATAAACTCAAATTTTAGTAGGCATATT
ATTACTATTGAGGATCCTATTGAGTATTTACATACTCACAAAAAGTCTATTGTTAACCAAAGAGAAGTAGGAAGCGATAC
CTATTCTTTTGCAAATGCTTTAAGGTCAGCTTTAAGAGAGGATCCTGATGTAATCCTCGTGGGTGAGATGCGAGACTTAG
AAACCACAGCTATAGCCATAACGGCAGCAGAGACAGGACACTTAGTAATGGCTACTCTGCATACTGTAGATGCTGTACAA
AGTATAGAAAGAATTGTAGACCAATTCCCTGCCCATCAGCAGCAACAGATAAGATTGCAACTTTCTGGAGTTATACAAGG
TATCATATCTCAACAACTTTTACCAAGAAAGGATGGCACAGGAAGAATTGTTGCAACAGAGATTTTAATTGGAATTCCAG
CTGTAAGAAGTTTGATAAGAGAAGGAAAAACCCCACAAATATATAGTGTAATTCAAACGGGGGGAAGATATGGAATGCAA
ACAATGGATCAAAGTCTTGCAGATCTTGTGAAAAAAGGACTTATTTCTCAAGAAGTAGCCTTTGAAAGGGCAGTGAATAG
AGAGGAATTAATCCGTCTTTTGGGGGGAGAAGTAAGGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7C3KPN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.091

98.056

0.55
  pilT Neisseria meningitidis 8013

52.601

96.111

0.506
  pilT Pseudomonas aeruginosa PAK

53.529

94.444

0.506
  pilT Neisseria gonorrhoeae MS11

52.023

96.111

0.5
  pilT Pseudomonas stutzeri DSM 10701

52.023

96.111

0.5
  pilT Acinetobacter baylyi ADP1

52.187

95.278

0.497
  pilT Legionella pneumophila strain Lp02

51.312

95.278

0.489
  pilT Acinetobacter baumannii strain A118

52.381

93.333

0.489
  pilT Legionella pneumophila strain ERS1305867

51.312

95.278

0.489
  pilT Acinetobacter baumannii D1279779

52.381

93.333

0.489
  pilT Acinetobacter nosocomialis M2

52.381

93.333

0.489
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.298

93.333

0.469
  pilT Vibrio cholerae strain A1552

50.298

93.333

0.469
  pilU Pseudomonas stutzeri DSM 10701

45.029

95

0.428
  pilU Vibrio cholerae strain A1552

43.353

96.111

0.417
  pilU Acinetobacter baylyi ADP1

44.214

93.611

0.414

Multiple sequence alignment