Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THEYE_RS10295 Genome accession   NC_011296
Coordinates   1986317..1987396 (-) Length   359 a.a.
NCBI ID   WP_012546105.1    Uniprot ID   B5YJ00
Organism   Thermodesulfovibrio yellowstonii DSM 11347     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1981317..1992396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEYE_RS10270 (THEYE_A2082) - 1981692..1982204 (-) 513 WP_012546721.1 flagellar basal body-associated protein FliL -
  THEYE_RS10275 (THEYE_A2083) mutL 1982512..1984146 (-) 1635 WP_012546175.1 DNA mismatch repair endonuclease MutL -
  THEYE_RS10280 (THEYE_A2084) - 1984153..1984704 (-) 552 WP_012545495.1 Slp family lipoprotein -
  THEYE_RS10285 (THEYE_A2085) - 1984750..1985145 (-) 396 WP_012546370.1 response regulator transcription factor -
  THEYE_RS10290 (THEYE_A2086) - 1985157..1986320 (-) 1164 WP_012545245.1 PilT/PilU family type 4a pilus ATPase -
  THEYE_RS10295 (THEYE_A2087) pilT 1986317..1987396 (-) 1080 WP_012546105.1 type IV pilus twitching motility protein PilT Machinery gene
  THEYE_RS10300 (THEYE_A2088) - 1987458..1988102 (+) 645 WP_012545723.1 HAD family hydrolase -
  THEYE_RS10305 (THEYE_A2089) - 1988104..1988847 (+) 744 WP_012546853.1 carbon monoxide dehydrogenase accessory protein CooC -
  THEYE_RS10310 (THEYE_A2090) mazG 1988840..1989610 (+) 771 WP_012545835.1 nucleoside triphosphate pyrophosphohydrolase -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 40076.43 Da        Isoelectric Point: 7.0295

>NTDB_id=31627 THEYE_RS10295 WP_012546105.1 1986317..1987396(-) (pilT) [Thermodesulfovibrio yellowstonii DSM 11347]
MARIDAFFKLMLENKASDLHLVAGSQPVLRIHGELVRVQYKVLDNDELRKFLYEITPEEKIKIFEETGDLDFAHEIPGVA
RFRVNYFMHKEGIGAAFRHIPNEIKTIDQLGLPVVLKKFAMLKKGIVLVTGPTGSGKSTTLAAIIDYANSNRKERIITIE
DPIEFVHPNKGCVISYREVGTHTESFAKALRAALREDPDIVLVGEMRDMETIQLALEAAATGHLVFSTLHTSSAIKTVDR
IIDVFPPEQQAQVRTSLSESLQAVVAQTLLKRADGKGRVAACEILINTYAVANLIREGKTHQILSIMQTSKKIGMQTMDD
TLLQLLQEGKIAPEDAYERATDKQKFVKFLKEVPQEGLT

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=31627 THEYE_RS10295 WP_012546105.1 1986317..1987396(-) (pilT) [Thermodesulfovibrio yellowstonii DSM 11347]
ATGGCTCGGATAGATGCCTTTTTTAAACTAATGCTTGAAAACAAAGCAAGTGATCTTCATCTTGTTGCTGGAAGTCAGCC
AGTTTTAAGAATTCACGGAGAGCTTGTCAGGGTTCAGTATAAAGTTCTTGATAATGATGAACTTAGGAAGTTTCTTTATG
AAATTACACCAGAGGAAAAAATCAAAATATTTGAGGAAACAGGAGATCTTGATTTTGCTCATGAAATACCTGGTGTTGCT
CGTTTTCGTGTTAACTATTTTATGCATAAGGAAGGAATTGGCGCAGCATTCAGGCACATTCCTAATGAAATAAAAACAAT
TGATCAGCTTGGGTTGCCAGTTGTATTAAAAAAGTTCGCAATGCTTAAAAAGGGAATTGTTCTTGTTACAGGTCCTACAG
GTTCAGGAAAATCAACAACCCTTGCTGCTATTATTGATTATGCAAACAGTAACAGAAAAGAAAGAATAATAACTATTGAA
GACCCCATAGAGTTTGTTCATCCGAATAAAGGCTGCGTTATCTCTTACAGAGAAGTAGGAACTCATACAGAAAGCTTTGC
GAAAGCCTTAAGAGCTGCTCTTAGAGAAGACCCAGATATAGTTTTGGTTGGTGAGATGAGAGACATGGAGACAATTCAGC
TTGCTCTGGAAGCTGCTGCTACAGGACATCTTGTATTCAGTACACTTCATACTTCATCAGCCATTAAAACAGTTGATAGA
ATTATTGATGTTTTTCCACCTGAACAGCAGGCGCAGGTAAGGACATCTCTAAGTGAGTCTTTGCAAGCAGTTGTTGCTCA
GACACTTTTAAAAAGGGCTGATGGTAAGGGAAGAGTTGCAGCATGTGAGATTTTGATTAACACCTATGCTGTTGCAAATC
TTATAAGAGAGGGAAAAACCCATCAAATACTCTCAATTATGCAGACAAGCAAAAAAATAGGCATGCAGACAATGGATGAT
ACTTTATTGCAACTTCTTCAGGAAGGCAAAATTGCACCAGAGGATGCCTATGAAAGAGCAACGGACAAGCAGAAATTTGT
TAAATTTCTTAAAGAAGTTCCTCAAGAAGGACTTACATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B5YJ00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

53.061

95.543

0.507
  pilT Legionella pneumophila strain ERS1305867

54.328

93.315

0.507
  pilT Legionella pneumophila strain Lp02

54.328

93.315

0.507
  pilT Acinetobacter nosocomialis M2

52.616

95.822

0.504
  pilT Acinetobacter baumannii D1279779

52.616

95.822

0.504
  pilT Acinetobacter baumannii strain A118

52.616

95.822

0.504
  pilT Pseudomonas aeruginosa PAK

54.079

92.201

0.499
  pilT Pseudomonas stutzeri DSM 10701

54.079

92.201

0.499
  pilT Neisseria meningitidis 8013

53.293

93.036

0.496
  pilT Neisseria gonorrhoeae MS11

52.994

93.036

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

97.493

0.482
  pilT Vibrio cholerae strain A1552

52.744

91.365

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.744

91.365

0.482
  pilU Pseudomonas stutzeri DSM 10701

41.62

99.721

0.415
  pilU Vibrio cholerae strain A1552

40.805

96.936

0.396
  pilU Acinetobacter baylyi ADP1

42.388

93.315

0.396

Multiple sequence alignment