Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ANAEK_RS03270 Genome accession   NC_011145
Coordinates   740089..741189 (+) Length   366 a.a.
NCBI ID   WP_012524726.1    Uniprot ID   B4UCS2
Organism   Anaeromyxobacter sp. K     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 735089..746189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANAEK_RS03245 (AnaeK_0652) - 735483..736331 (+) 849 WP_012524721.1 purine-nucleoside phosphorylase -
  ANAEK_RS03250 (AnaeK_0653) - 736379..736732 (+) 354 WP_012524722.1 PilZ domain-containing protein -
  ANAEK_RS03255 (AnaeK_0654) - 736737..737141 (+) 405 WP_012524723.1 cytidine deaminase -
  ANAEK_RS03260 (AnaeK_0655) ribF 737215..738186 (+) 972 WP_012524724.1 riboflavin biosynthesis protein RibF -
  ANAEK_RS03265 (AnaeK_0656) pilB 738369..740075 (+) 1707 WP_012524725.1 type IV-A pilus assembly ATPase PilB -
  ANAEK_RS03270 (AnaeK_0657) pilT 740089..741189 (+) 1101 WP_012524726.1 type IV pilus twitching motility protein PilT Machinery gene
  ANAEK_RS03275 (AnaeK_0658) - 741258..742511 (+) 1254 WP_012524727.1 type II secretion system F family protein -
  ANAEK_RS03280 (AnaeK_0659) - 742522..744099 (+) 1578 WP_012524728.1 ATP-binding protein -
  ANAEK_RS03285 (AnaeK_0660) - 744112..745494 (+) 1383 WP_012524729.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40078.49 Da        Isoelectric Point: 9.2811

>NTDB_id=31430 ANAEK_RS03270 WP_012524726.1 740089..741189(+) (pilT) [Anaeromyxobacter sp. K]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRIDGKLVPLKTPPLSPVETKQLCYSILTDAQKHKFEEESELDLSFGVKGLS
RFRANVFMQRGAVAGAFRTIPFKILTFQELGLPPIVAELAKKPRGLVLVTGPTGSGKSTTLASIIDKINTDRHEHIITIE
DPIEYLHPHKNCVVNQREVGADTASFKKALKYILRQDPDVVLVGEMRDLETIEAALVISETGHLAFATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVLTQNLLPKAGGPGRVLIIEVMVPNPAIRNLIREDKVHQVYSQMQVGQAKFGMQTFN
QSLAMAFAKRLITLEEALGRSSDAEELKNIIASGAALRGPPQGAAR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=31430 ANAEK_RS03270 WP_012524726.1 740089..741189(+) (pilT) [Anaeromyxobacter sp. K]
TTGGCGAACCTGCACCAGCTCCTGAAGGCGATGGTCGAGAAGGGGGCCTCCGACCTCCACGTCACCACCGGCTCCCCGCC
CCAGCTCCGCATCGACGGCAAGCTGGTGCCGCTGAAGACCCCGCCGCTCTCGCCGGTGGAGACGAAGCAGCTCTGCTACT
CGATCCTCACCGACGCGCAGAAGCACAAGTTCGAGGAGGAGAGCGAGCTCGACCTCTCCTTCGGCGTGAAGGGGCTCTCG
CGCTTCCGCGCGAACGTCTTCATGCAGCGCGGCGCGGTGGCCGGCGCGTTCCGCACCATCCCGTTCAAGATCCTCACCTT
CCAGGAGCTGGGCCTGCCGCCCATCGTGGCCGAGCTGGCCAAGAAGCCGCGCGGCCTGGTGCTCGTCACGGGCCCCACCG
GCTCCGGCAAGTCCACCACGCTCGCGTCGATCATCGACAAGATCAACACCGACCGGCACGAGCACATCATCACGATCGAG
GATCCGATCGAGTACCTGCACCCGCACAAGAACTGCGTGGTGAACCAGCGCGAGGTCGGGGCGGACACCGCCTCGTTCAA
GAAGGCGCTGAAGTACATCCTCCGCCAGGATCCCGACGTCGTGCTCGTCGGCGAGATGCGCGACCTCGAGACCATCGAGG
CGGCGCTGGTGATCTCCGAGACCGGCCACCTCGCCTTCGCGACGCTGCACACCAACAGCGCGGTCCAGACGATCAACCGC
ATCCTGGACGTGTTCCCGCCCTACCAGCAGCCGCAGGTCCGCGCGCAGCTCTCGTTCGTGCTGGAGGGCGTGCTCACCCA
GAACCTGCTGCCCAAGGCCGGCGGCCCCGGCCGCGTGCTCATCATCGAGGTGATGGTCCCGAACCCCGCGATCCGGAACC
TCATCCGCGAGGACAAGGTGCACCAGGTCTACTCGCAGATGCAGGTCGGCCAGGCCAAGTTCGGCATGCAGACCTTCAAC
CAGAGCCTGGCGATGGCGTTCGCGAAGCGCCTCATCACGCTGGAGGAGGCGCTCGGCCGGTCCTCCGACGCGGAGGAGCT
GAAGAACATCATCGCGAGCGGCGCCGCGCTGCGCGGCCCGCCACAAGGTGCGGCGCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4UCS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.977

96.721

0.503
  pilT Legionella pneumophila strain Lp02

53.061

93.716

0.497
  pilT Legionella pneumophila strain ERS1305867

53.061

93.716

0.497
  pilT Acinetobacter baylyi ADP1

52.537

91.53

0.481
  pilT Pseudomonas aeruginosa PAK

51.94

91.53

0.475
  pilT Acinetobacter baumannii D1279779

51.343

91.53

0.47
  pilT Acinetobacter baumannii strain A118

51.343

91.53

0.47
  pilT Acinetobacter nosocomialis M2

51.045

91.53

0.467
  pilT Pseudomonas stutzeri DSM 10701

51.045

91.53

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

91.257

0.451
  pilT Vibrio cholerae strain A1552

49.401

91.257

0.451
  pilT Neisseria gonorrhoeae MS11

46.921

93.169

0.437
  pilT Neisseria meningitidis 8013

46.921

93.169

0.437
  pilU Pseudomonas stutzeri DSM 10701

42.135

97.268

0.41
  pilU Vibrio cholerae strain A1552

41.16

98.907

0.407
  pilU Acinetobacter baylyi ADP1

39.481

94.809

0.374

Multiple sequence alignment