Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D3A95_RS09820 Genome accession   NZ_CP032152
Coordinates   1989050..1990144 (-) Length   364 a.a.
NCBI ID   WP_181494854.1    Uniprot ID   -
Organism   Thermosynechococcus sichuanensis E542     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1984050..1995144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3A95_RS09780 (D3A95_09910) - 1984442..1984666 (+) 225 WP_181494847.1 spore coat protein U domain-containing protein -
  D3A95_RS09785 (D3A95_09915) - 1984663..1985034 (-) 372 WP_181494848.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  D3A95_RS09795 (D3A95_09925) - 1985249..1986400 (+) 1152 WP_181494849.1 cysteine desulfurase family protein -
  D3A95_RS09800 (D3A95_09930) - 1986693..1987046 (+) 354 WP_181494850.1 hypothetical protein -
  D3A95_RS09805 (D3A95_09935) - 1987043..1987315 (-) 273 WP_181494851.1 DUF3143 domain-containing protein -
  D3A95_RS09810 (D3A95_09940) - 1987312..1987785 (-) 474 WP_181494852.1 J domain-containing protein -
  D3A95_RS09815 (D3A95_13305) - 1987794..1989014 (-) 1221 WP_181494853.1 type II secretion system F family protein -
  D3A95_RS09820 (D3A95_09950) pilT 1989050..1990144 (-) 1095 WP_181494854.1 type IV pilus twitching motility protein PilT Machinery gene
  D3A95_RS09825 (D3A95_09955) pilB/pilB1 1990213..1992225 (-) 2013 WP_181494855.1 GspE/PulE family protein Machinery gene
  D3A95_RS09830 (D3A95_09960) - 1992487..1992699 (+) 213 WP_181494856.1 hypothetical protein -
  D3A95_RS09835 (D3A95_09965) - 1992700..1992897 (+) 198 WP_181494857.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  D3A95_RS09840 (D3A95_09970) - 1992945..1994435 (+) 1491 WP_181494858.1 right-handed parallel beta-helix repeat-containing protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40285.39 Da        Isoelectric Point: 6.7533

>NTDB_id=314126 D3A95_RS09820 WP_181494854.1 1989050..1990144(-) (pilT) [Thermosynechococcus sichuanensis E542]
MELMIEDLMEQVVANGGSDLHISAGLPPYIRISGKLTPTDYEPLTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMCERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVMGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIITYEAAMSKSSRQDELQRLIGAGTTAAAR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=314126 D3A95_RS09820 WP_181494854.1 1989050..1990144(-) (pilT) [Thermosynechococcus sichuanensis E542]
ATGGAATTAATGATTGAAGACCTAATGGAGCAAGTGGTGGCCAATGGTGGCTCCGATTTGCACATCTCCGCTGGCTTACC
CCCCTACATTCGCATTAGTGGCAAATTAACACCGACGGACTACGAGCCACTCACCCCAGAGCAATGCCAACGCCTGATCT
TCAGCATGCTCAACAACACCCAACGCAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGTGTACGGGGTCTA
GCCCGCTTCCGCGTCAATGTCTATAAAGACCGGGGTACCTATGCGGCCTGCTTACGAGCACTCAGTTCCAAAATTCCGAC
GTTTGAGCAGTTGGGATTGCCCAATATTGTCCGCGAGATGTGTGAGCGACCCCGAGGCCTGATTCTGGTCACTGGACCGA
CGGGATCAGGGAAAACCACGACTTTGGCGGCGATGATTGATCTGATCAACAAAACCCGTGCCGAGCATATTTTGACGATT
GAGGACCCGATTGAATTTGTTTACGAACCGATCAAGAGCCTGATCCACCAACGGCAGGTGGGGGAAGACACCAAGAGCTT
TGCCAATGCCCTGCGGGCAGCACTGCGGGAAGACCCGGACATTATCCTAGTGGGTGAGATGCGTGACTTGGAAACCATTC
AATTAGCCATTTCGGCAGCGGAAACGGGTCACTTGGTGATGGGAACCCTGCACACGAGTTCAGCCGCCCAAACCGTTGAC
CGCATGGTGGATGTGTTCCCGCCAGAGCAGCAGCAGCAAATTCGCGTCCAGTTATCCAACTCTCTAGTGGCGGTCTTTAG
CCAAACACTGGTGCCCAAGAAAAATCCCAAGCCCGGTGAATTTGGGCGGATTATGGCGCAAGAGATTATGGTGGTGACCC
CTGCTATTTCCAACCTGATTCGCGAAGGCAAGACCTCGCAGATCTATTCAGCCATTCAAACGGGGGGCAAGCTGGGAATG
CAGACTCTGGAAAAAGTCTTGGCCGATTATTATCGTGCCGGCATCATTACCTATGAAGCAGCAATGTCTAAATCCTCGCG
TCAGGATGAGTTGCAACGTCTCATTGGTGCCGGTACAACTGCGGCGGCGCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.839

93.681

0.514
  pilT Legionella pneumophila strain Lp02

53.913

94.78

0.511
  pilT Legionella pneumophila strain ERS1305867

53.913

94.78

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.644

94.231

0.505
  pilT Acinetobacter baylyi ADP1

54.599

92.582

0.505
  pilT Acinetobacter baumannii strain A118

54.006

92.582

0.5
  pilT Acinetobacter nosocomialis M2

54.006

92.582

0.5
  pilT Acinetobacter baumannii D1279779

54.006

92.582

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.571

96.154

0.486
  pilT Neisseria gonorrhoeae MS11

48.997

95.879

0.47
  pilT Neisseria meningitidis 8013

48.997

95.879

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.407

0.467
  pilT Vibrio cholerae strain A1552

50

93.407

0.467
  pilU Pseudomonas stutzeri DSM 10701

39.155

97.527

0.382
  pilU Acinetobacter baylyi ADP1

37.607

96.429

0.363

Multiple sequence alignment