Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   D3A95_RS09825 Genome accession   NZ_CP032152
Coordinates   1990213..1992225 (-) Length   670 a.a.
NCBI ID   WP_181494855.1    Uniprot ID   -
Organism   Thermosynechococcus sichuanensis E542     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1985213..1997225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3A95_RS09795 (D3A95_09925) - 1985249..1986400 (+) 1152 WP_181494849.1 cysteine desulfurase family protein -
  D3A95_RS09800 (D3A95_09930) - 1986693..1987046 (+) 354 WP_181494850.1 hypothetical protein -
  D3A95_RS09805 (D3A95_09935) - 1987043..1987315 (-) 273 WP_181494851.1 DUF3143 domain-containing protein -
  D3A95_RS09810 (D3A95_09940) - 1987312..1987785 (-) 474 WP_181494852.1 J domain-containing protein -
  D3A95_RS09815 (D3A95_13305) - 1987794..1989014 (-) 1221 WP_181494853.1 type II secretion system F family protein -
  D3A95_RS09820 (D3A95_09950) pilT 1989050..1990144 (-) 1095 WP_181494854.1 type IV pilus twitching motility protein PilT Machinery gene
  D3A95_RS09825 (D3A95_09955) pilB/pilB1 1990213..1992225 (-) 2013 WP_181494855.1 GspE/PulE family protein Machinery gene
  D3A95_RS09830 (D3A95_09960) - 1992487..1992699 (+) 213 WP_181494856.1 hypothetical protein -
  D3A95_RS09835 (D3A95_09965) - 1992700..1992897 (+) 198 WP_181494857.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  D3A95_RS09840 (D3A95_09970) - 1992945..1994435 (+) 1491 WP_181494858.1 right-handed parallel beta-helix repeat-containing protein -
  D3A95_RS09845 (D3A95_09975) - 1994432..1995418 (-) 987 WP_181494859.1 SPOR domain-containing protein -
  D3A95_RS09850 (D3A95_09980) - 1995528..1996466 (+) 939 WP_181494860.1 glycosyltransferase -
  D3A95_RS09855 (D3A95_09985) leuD 1996459..1997049 (-) 591 WP_181494861.1 3-isopropylmalate dehydratase small subunit -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74057.32 Da        Isoelectric Point: 5.3339

>NTDB_id=314127 D3A95_RS09825 WP_181494855.1 1990213..1992225(-) (pilB/pilB1) [Thermosynechococcus sichuanensis E542]
MVFSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEMTGTTMPPDVLRQYHKQQLFELKVIY
GVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHPEADPPYLLVAMVDPDNLQAIDNLTRLLRAHSLTLKRMV
ITLEDYQRLIDPILNKQVAETAASKNAPAAIGEINIEEDIEAIGGLEELEGEQEVDLAEALRGAEDAPIIALVNKILAKA
LTEGVSDIHVEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDFRVNT
LPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMIKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNISTAED
PIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLSEM
GIEPFMVSASLIGVVAQRLMRRVCTECRIPYQPTREELARFGLSASETSNITFYKANTLTPEQIQAAKASGQPICSKCGG
LGYKGRCGVYEIMRVTERLQSLITEGAPTERIKEAAVEEGMKTLLAYSLNLVKEGVTTLEEVERVTFTDTGLESELKAKR
KSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=314127 D3A95_RS09825 WP_181494855.1 1990213..1992225(-) (pilB/pilB1) [Thermosynechococcus sichuanensis E542]
ATGGTATTTTCCTCTCGCAAAGCCCTTACCGTACGTGGCAGAGCCGCCAGCCCCACAGAGCGAGCCATTGTTGCCTCCGG
TTACGCCAGCATTGATCAAGTGCGGGAAGCCATGAACACCGCCCGCAAAACAGGCAAGTCCCTCGTCACCGTCTTGCAGG
AGATGACGGGCACCACCATGCCCCCCGATGTGTTGCGCCAGTACCACAAGCAACAGCTTTTTGAACTCAAGGTTATCTAC
GGTGTCGATTGTCTCGATCCCGAACTCAATCGCTTCCCCACCGAGCAAATCGAGGAACTGATCAACACGATTGTACCGAT
TGACACCTGTCGCACTTACCAAGTCATTCCCATTGCTAAGCACCCTGAGGCTGACCCCCCCTATCTCCTCGTGGCGATGG
TGGATCCCGATAACCTTCAAGCCATTGACAACCTCACCCGCTTGCTCCGCGCCCACAGCCTCACCCTCAAGCGGATGGTG
ATCACCCTAGAGGACTACCAACGCCTCATTGATCCCATCCTCAACAAACAGGTGGCGGAAACTGCTGCCAGCAAAAATGC
CCCCGCTGCGATTGGTGAAATCAACATTGAAGAAGATATTGAAGCCATAGGGGGACTCGAAGAGCTAGAAGGAGAGCAGG
AGGTTGACCTTGCTGAAGCTCTGAGGGGGGCAGAAGACGCACCGATTATTGCCCTTGTGAATAAAATTTTGGCCAAAGCG
CTCACCGAAGGCGTTTCCGATATTCACGTTGAACCGCAAGAGGAATACCTACGGATTCGTTTCCGCAAAGATGGGGTGCT
CCACCAAGCCTTTGATCCCCTGCCGAAGAAGATCGTGCCCGCCGTGGTTTCCCGCTTCAAAATCTTGGCTGACCTCGATA
TTGCCGAGCGGCGTGCCCCCCAAGATGGTCGTATTCGCAAAATGTTCCAAGGGCGACGGGTGGACTTTCGGGTGAATACC
CTGCCCAGCCGCTGGGGCGAAAAAGTGGTACTGCGGATTCTCGATAACTCCGCTACCCAACTGGGTCTCGACAAACTCAT
TACCGACCCAGAGAGCCTTGCCATTGTTCGTGAGATGATCAAGCGTCCCTTTGGGTTGATCCTCGTTACAGGACCGACGG
GTTCAGGGAAAACCACCACACTGTACTCGGCGCTGGCCGAATGTAATAGCCCCGGTGTCAACATCAGTACAGCGGAAGAC
CCCATTGAATACACGCTCCCCGGCCTAACACAGGTGCAGGTCATTCGCGAAAAGGGCATGGACTTTGCCTCCATTCTGCG
TGCCTTTCTCCGCCAAGACCCCGATGTGATTCTCGTGGGGGAAACTCGCGACAAGGAAACCGCCAAAACCGCCATTGAAG
CGGCGTTGACAGGTCACTTGGTGTTGACCACCCTACACACCAACGATGCGGCCAGTGCCGTTGCTCGTCTCTCGGAAATG
GGGATCGAACCCTTCATGGTCTCCGCTTCCTTAATTGGCGTCGTTGCCCAGCGACTCATGCGGCGAGTTTGCACAGAATG
CCGTATTCCTTATCAACCGACCCGTGAAGAGTTAGCTCGTTTTGGTCTGTCCGCTTCGGAAACGAGCAATATTACCTTTT
ACAAAGCAAATACATTAACACCCGAGCAAATTCAGGCGGCTAAGGCCAGTGGTCAACCCATTTGTAGTAAATGCGGAGGA
TTGGGCTACAAGGGTCGCTGTGGGGTCTATGAAATTATGCGGGTGACGGAGCGCCTGCAAAGCCTGATTACTGAAGGTGC
CCCCACCGAGCGGATCAAGGAAGCGGCGGTTGAAGAGGGGATGAAAACCCTCTTGGCCTATAGCCTGAACTTGGTTAAGG
AAGGCGTCACCACCCTCGAAGAAGTCGAGCGCGTCACCTTTACGGACACGGGTCTCGAATCAGAACTCAAGGCCAAACGT
AAGAGTTCTTTAACTTGCCGTACCTGTGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTGCATGACACCACG
CTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.701

97.91

0.643

Multiple sequence alignment