Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HY04AAS1_RS01250 Genome accession   NC_011126
Coordinates   218271..219350 (-) Length   359 a.a.
NCBI ID   WP_012513294.1    Uniprot ID   -
Organism   Hydrogenobaculum sp. Y04AAS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 213271..224350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HY04AAS1_RS01225 (HY04AAS1_0239) - 213623..213838 (-) 216 WP_012513288.1 hypothetical protein -
  HY04AAS1_RS01230 (HY04AAS1_0240) - 213835..214746 (-) 912 WP_012513289.1 MoxR family ATPase -
  HY04AAS1_RS01235 (HY04AAS1_0241) - 214767..216689 (-) 1923 WP_012513290.1 GGDEF domain-containing protein -
  HY04AAS1_RS01240 (HY04AAS1_0243) - 216986..217282 (+) 297 WP_337954080.1 hypothetical protein -
  HY04AAS1_RS01245 (HY04AAS1_0244) recR 217272..217943 (+) 672 WP_012513293.1 recombination mediator RecR -
  HY04AAS1_RS01250 (HY04AAS1_0245) pilT 218271..219350 (-) 1080 WP_012513294.1 type IV pilus twitching motility protein PilT Machinery gene
  HY04AAS1_RS01255 (HY04AAS1_0246) - 219379..221061 (-) 1683 WP_012513295.1 GspE/PulE family protein -
  HY04AAS1_RS08410 (HY04AAS1_0247) - 221064..221309 (-) 246 WP_337954098.1 DNA topoisomerase -
  HY04AAS1_RS01260 (HY04AAS1_0249) hpt 221582..222085 (-) 504 WP_012513296.1 hypoxanthine phosphoribosyltransferase -
  HY04AAS1_RS01280 (HY04AAS1_0250) tuf 222513..223730 (+) 1218 WP_012513297.1 elongation factor Tu -
  HY04AAS1_RS08350 (HY04AAS1_0251) rpmG 223741..223890 (+) 150 WP_012513298.1 50S ribosomal protein L33 -
  HY04AAS1_RS01290 (HY04AAS1_0252) secE 223990..224190 (+) 201 WP_012513299.1 preprotein translocase subunit SecE -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 40128.40 Da        Isoelectric Point: 7.2023

>NTDB_id=31342 HY04AAS1_RS01250 WP_012513294.1 218271..219350(-) (pilT) [Hydrogenobaculum sp. Y04AAS1]
MQLDIGTLTEILVKNKGSDIHIVAGSKPAIRADGKIKFLEEYNTLTPEDTQHLAYGIMLEKYKKVFEEKNAVDFSIGVHG
LGRFRVNVFRQRDSVSMVLRYLTSEIKDITKLGLDTKVLELTKRSMGLILVTGPTGSGKSTTLASMIQYILDNEPVHVIT
VEDPIEYLFKHSKGIVNQRELGQDVPSFSDALRSALREDPDVILVGEMRDRETIEMALRAAETGHLVFGTLHTNTAISTI
NRIVDVFSAEEKDQIRTQLSISLQGIISQRLLPKINGGRVLAYELLIPNTGIRNLIKENKLPQAYALMQSGQAQTGMQTM
NQHLRKLYGMGLITKEDALKYSPDPKELARLMGVAYEEM

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=31342 HY04AAS1_RS01250 WP_012513294.1 218271..219350(-) (pilT) [Hydrogenobaculum sp. Y04AAS1]
ATGCAACTTGATATAGGGACGTTAACAGAAATACTGGTAAAAAACAAAGGAAGTGATATTCACATAGTGGCAGGCTCTAA
ACCTGCTATAAGAGCAGATGGCAAAATAAAGTTTTTAGAAGAATACAATACATTAACACCTGAAGATACACAGCATTTAG
CCTACGGCATTATGCTTGAGAAATATAAAAAAGTATTTGAAGAGAAAAATGCAGTAGATTTTTCTATCGGAGTACACGGT
TTAGGCAGATTTCGCGTAAATGTATTTAGACAAAGAGACTCTGTAAGTATGGTTTTAAGATACTTAACCAGCGAGATAAA
AGATATTACAAAATTAGGGTTGGACACAAAGGTCTTAGAACTTACCAAAAGGAGTATGGGACTTATTCTAGTAACAGGTC
CAACAGGTAGCGGTAAATCCACGACCTTAGCATCGATGATACAGTATATACTTGATAATGAGCCTGTACATGTTATAACA
GTGGAAGATCCGATAGAATATCTTTTTAAACATAGTAAAGGTATTGTAAACCAGCGCGAGTTAGGACAAGATGTACCAAG
TTTTTCCGATGCTTTAAGAAGCGCTTTAAGAGAAGACCCAGATGTAATACTAGTAGGTGAAATGAGAGATAGAGAAACTA
TAGAAATGGCTTTAAGAGCAGCTGAAACAGGGCACTTGGTATTTGGAACACTTCATACAAATACCGCTATATCCACTATA
AATAGAATTGTAGATGTATTTAGTGCAGAAGAAAAGGATCAAATAAGGACACAGCTCTCTATCTCTTTGCAAGGTATTAT
ATCTCAAAGGCTTTTACCCAAAATAAACGGTGGTAGAGTGTTAGCTTACGAGCTTTTAATACCAAACACAGGTATAAGGA
ATTTGATAAAAGAAAATAAGCTACCACAAGCTTACGCTTTGATGCAATCAGGTCAAGCTCAAACAGGTATGCAAACCATG
AATCAACACTTAAGAAAACTATACGGTATGGGGCTTATAACAAAAGAAGATGCTCTAAAATACAGTCCAGATCCAAAAGA
ATTGGCAAGGTTAATGGGTGTAGCTTATGAAGAGATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

51.786

93.593

0.485
  pilT Legionella pneumophila strain Lp02

51.786

93.593

0.485
  pilT Acinetobacter baumannii D1279779

49.133

96.379

0.474
  pilT Acinetobacter baumannii strain A118

49.133

96.379

0.474
  pilT Acinetobacter nosocomialis M2

49.133

96.379

0.474
  pilT Acinetobacter baylyi ADP1

49.133

96.379

0.474
  pilT Pseudomonas aeruginosa PAK

48.555

96.379

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.714

97.493

0.465
  pilT Pseudomonas stutzeri DSM 10701

47.977

96.379

0.462
  pilT Neisseria meningitidis 8013

45.977

96.936

0.446
  pilT Neisseria gonorrhoeae MS11

45.69

96.936

0.443
  pilT Vibrio cholerae strain A1552

46.866

93.315

0.437
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.866

93.315

0.437
  pilU Pseudomonas stutzeri DSM 10701

41.954

96.936

0.407
  pilU Acinetobacter baylyi ADP1

39.08

96.936

0.379
  pilU Vibrio cholerae strain A1552

38.304

95.265

0.365

Multiple sequence alignment