Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AhaeINNSZ174_RS05250 Genome accession   NZ_CP031998
Coordinates   1097293..1098330 (-) Length   345 a.a.
NCBI ID   WP_160132419.1    Uniprot ID   -
Organism   Acinetobacter haemolyticus strain INNSZ174     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1092293..1103330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AhaeINNSZ174_RS05220 (AhaeINNSZ174_05220) - 1092947..1093402 (+) 456 WP_008940857.1 bacteriohemerythrin -
  AhaeINNSZ174_RS05225 (AhaeINNSZ174_05225) - 1093652..1094734 (+) 1083 WP_005091350.1 membrane protein -
  AhaeINNSZ174_RS05230 (AhaeINNSZ174_05230) - 1094734..1095054 (+) 321 WP_005091348.1 RnfH family protein -
  AhaeINNSZ174_RS05235 (AhaeINNSZ174_05235) - 1095111..1095509 (-) 399 WP_004638212.1 outer membrane protein assembly factor BamE -
  AhaeINNSZ174_RS05240 (AhaeINNSZ174_05240) fur 1095621..1096058 (+) 438 WP_004638213.1 ferric iron uptake transcriptional regulator -
  AhaeINNSZ174_RS05245 (AhaeINNSZ174_05245) pilU 1096146..1097264 (-) 1119 WP_005081584.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AhaeINNSZ174_RS05250 (AhaeINNSZ174_05250) pilT 1097293..1098330 (-) 1038 WP_160132419.1 type IV pilus twitching motility protein PilT Machinery gene
  AhaeINNSZ174_RS05255 (AhaeINNSZ174_05255) - 1098457..1099149 (+) 693 WP_005091345.1 YggS family pyridoxal phosphate-dependent enzyme -
  AhaeINNSZ174_RS05260 (AhaeINNSZ174_05260) - 1099156..1099827 (-) 672 WP_008940858.1 HAD family hydrolase -
  AhaeINNSZ174_RS05265 (AhaeINNSZ174_05265) - 1099899..1100171 (-) 273 WP_005091337.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38342.93 Da        Isoelectric Point: 6.3475

>NTDB_id=313251 AhaeINNSZ174_RS05250 WP_160132419.1 1097293..1098330(-) (pilT) [Acinetobacter haemolyticus strain INNSZ174]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGTIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHDIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KSLVSKGLISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=313251 AhaeINNSZ174_RS05250 WP_160132419.1 1097293..1098330(-) (pilT) [Acinetobacter haemolyticus strain INNSZ174]
ATGGATATCACAGAATTACTCGCCTTTTCAGTAAAAAATGGTGCATCGGATTTACACCTTTCGGCGGGAATGCCACCTAT
GATTCGTGTAGATGGTGAGGTTCGCCGAATTAATCTACCAGCTCTAGAGCATAAAGATGTCCATCGTCTTGTTTATGACA
TCATGAACGATAAGCAACGTCGTGATTTTGAGGAAGATCTTGAAACTGACTTCTCTTTTGAAGTACCCAATATCGCACGT
TTCCGTGTCAACGCATTCAACCAAAACCGTGGTGCGGGTGCCGTATTCCGTACCATTCCATCCAAAGTTTTAACCATGGA
AGACTTGGGTTTAGGCACAATTTTCAAAGAAATTTGTGATTATCCGCGAGGAATCGTATTGGTCACTGGACCTACAGGTT
CGGGTAAATCGACAACTCTCGCAGCAATGATTGATTACATCAATGAAAATCGTTACGACCATATCCTAACGGTTGAAGAT
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTGATTAACCAACGTGAAGTGCATCGTGATACCCATGGTTTTAACGA
AGCATTACGTTCTGCACTGCGTGAAGACCCTGACATTATTCTGGTCGGTGAGATGCGTGACTTAGAGACCATTCGCCTTG
CACTCACCGCAGCGGAAACAGGTCACCTTGTATTTGGTACACTGCATACCACCTCAGCAGCAAAAACCATTGACCGTGTG
ATCGACGTATTCCCAGCCGAGGAAAAAGACATGGTTCGTGCCATGCTCTCAGAATCATTACAAGCGGTAATTTCTCAAAC
CTTATTGAAAAAGAATGGCGGCGGTCGTGTGGCAGCACATGATATCATGATCGGGATTCCTGCGATCCGTAACCTCGTCC
GTGAAAACAAAGTCGCACAAATGTATTCTGCGATCCAAACAGGAGCGAACTATGGCATGACGACTTTAGATCAAAGTCTA
AAAAGTCTGGTATCTAAAGGTCTTATTAGTCCACAAACCGCACGTACAGTTGCAAAGCAACCAGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment