Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D0N50_RS04010 Genome accession   NZ_CP031695
Coordinates   875886..876893 (+) Length   335 a.a.
NCBI ID   WP_151036615.1    Uniprot ID   -
Organism   Erwinia billingiae strain TH88     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 870886..881893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0N50_RS03975 (D0N50_03995) trmB 870933..871652 (+) 720 WP_013203693.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  D0N50_RS03980 (D0N50_04000) - 871652..871978 (+) 327 WP_013203692.1 YggL family protein -
  D0N50_RS03985 (D0N50_04005) - 872076..872789 (+) 714 WP_151036612.1 DUF2884 domain-containing protein -
  D0N50_RS03990 (D0N50_04010) hemW 872843..873991 (-) 1149 WP_151036613.1 radical SAM family heme chaperone HemW -
  D0N50_RS03995 (D0N50_04015) - 873984..874577 (-) 594 WP_105760127.1 XTP/dITP diphosphatase -
  D0N50_RS04000 (D0N50_04020) - 874599..875153 (-) 555 WP_013203688.1 YggT family protein -
  D0N50_RS04005 (D0N50_04025) - 875162..875869 (-) 708 WP_151036614.1 YggS family pyridoxal phosphate-dependent enzyme -
  D0N50_RS04010 (D0N50_04030) pilT 875886..876893 (+) 1008 WP_151036615.1 type IV pilus twitching motility protein PilT Machinery gene
  D0N50_RS04015 (D0N50_04035) ruvX 876910..877332 (-) 423 WP_191907175.1 Holliday junction resolvase RuvX -
  D0N50_RS04020 (D0N50_04040) - 877332..877895 (-) 564 WP_151036617.1 YqgE/AlgH family protein -
  D0N50_RS04025 (D0N50_04045) gshB 877990..878934 (-) 945 WP_151036618.1 glutathione synthase -
  D0N50_RS04030 (D0N50_04050) rsmE 878974..879705 (-) 732 WP_013203682.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D0N50_RS04035 (D0N50_04055) endA 879780..880493 (-) 714 WP_151036619.1 deoxyribonuclease I -
  D0N50_RS04040 (D0N50_04060) - 880599..881123 (-) 525 WP_151036620.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36323.51 Da        Isoelectric Point: 6.5608

>NTDB_id=310442 D0N50_RS04010 WP_151036615.1 875886..876893(+) (pilT) [Erwinia billingiae strain TH88]
MDIEDMVALSVKHNASDLHLCSGHSPFWRRCGQLEPIGALPVVSSAMLESHARHWLTDAQQAAFAEAGQLDFALTMADGT
RLRANLFRQRQGHSLALRAIASESPDMATLKLPEITRSLLQLQAGLVLVTGATGSGKSTTLASMIAAINQQQARHILTLE
DPIEFLHHSQRSLIQQREIGTHCASFSEGLRSALREDPDVILLGELRDGDTIQQALTAAETGHLVLATLHTRGATQAVDR
LVDVFPGAEKNLIRSQLAGSLKAVIAQRLEPEGEGRIALFEVLVNTPAVANLIREGKTHQLGGLLETGSQVGMQTFAQCL
RQRQQAGMLLQLAWE

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=310442 D0N50_RS04010 WP_151036615.1 875886..876893(+) (pilT) [Erwinia billingiae strain TH88]
ATGGATATTGAGGACATGGTGGCCCTTAGTGTAAAGCATAACGCCTCGGATCTGCACCTTTGCAGTGGTCATTCTCCATT
CTGGCGCCGTTGCGGCCAACTGGAGCCGATCGGCGCTTTACCGGTGGTCAGCAGCGCGATGCTGGAAAGCCATGCGCGGC
ACTGGCTGACCGACGCCCAGCAGGCGGCATTCGCAGAGGCCGGCCAGCTGGACTTTGCCCTGACCATGGCGGACGGAACG
CGGTTGCGCGCCAACCTGTTCCGCCAGCGGCAGGGGCATTCACTGGCGTTGCGGGCGATCGCAAGCGAAAGCCCGGATAT
GGCCACGCTGAAGTTGCCGGAAATTACCCGCTCGCTGCTTCAGCTGCAGGCCGGGCTGGTGCTGGTCACCGGGGCAACCG
GCAGCGGAAAGTCCACCACGCTGGCGTCAATGATTGCCGCCATCAACCAACAGCAGGCGCGGCATATTCTGACGCTGGAA
GATCCCATCGAGTTTCTGCATCACAGCCAACGTTCGCTGATTCAGCAGCGCGAAATCGGCACGCATTGCGCATCCTTCAG
CGAAGGGTTGCGCAGCGCGCTGCGTGAAGACCCCGATGTCATCCTGCTGGGTGAACTGCGCGACGGCGATACCATTCAGC
AGGCATTAACCGCTGCGGAAACCGGGCATCTGGTGCTGGCAACGCTGCATACGCGAGGCGCAACGCAGGCCGTGGATCGG
CTGGTGGATGTGTTTCCGGGAGCGGAGAAAAACCTGATCCGCAGCCAGCTGGCCGGGAGCCTGAAAGCGGTGATCGCGCA
ACGGCTGGAGCCGGAAGGCGAGGGCCGCATCGCACTGTTCGAAGTGTTGGTTAATACGCCAGCGGTCGCGAACCTGATCC
GTGAGGGCAAAACCCATCAGCTTGGCGGATTACTGGAAACGGGATCGCAGGTCGGCATGCAAACCTTTGCTCAGTGCCTC
AGACAAAGGCAACAAGCGGGAATGCTGCTTCAGCTGGCATGGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.64

98.806

0.481
  pilT Neisseria gonorrhoeae MS11

48.338

98.806

0.478
  pilT Vibrio cholerae strain A1552

47.988

96.418

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.988

96.418

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.667

98.507

0.46
  pilT Acinetobacter baylyi ADP1

45.758

98.507

0.451
  pilT Pseudomonas aeruginosa PAK

45.758

98.507

0.451
  pilT Legionella pneumophila strain Lp02

46.44

96.418

0.448
  pilT Legionella pneumophila strain ERS1305867

46.44

96.418

0.448
  pilT Acinetobacter nosocomialis M2

45.963

96.119

0.442
  pilT Acinetobacter baumannii D1279779

45.963

96.119

0.442
  pilT Acinetobacter baumannii strain A118

45.963

96.119

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.031

96.119

0.433
  pilU Vibrio cholerae strain A1552

42.308

93.134

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.994

96.119

0.394
  pilU Acinetobacter baylyi ADP1

39.009

96.418

0.376

Multiple sequence alignment