Detailed information NTG0030810

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: EMIN_RS09295
• Genome accession: NC_010644
• Coordinates: 84711..85265 (-)
• Length: 184 a.a.
• NCBI ID: WP_202943288.1
• Uniprot ID: -
• Organism: Elusimicrobium minutum Pei191
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 79711..90265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EMIN_RS00380 (Emin_0075)	lpxD	79856..80881 (+)	1026	WP_012414257.1	UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase	-
EMIN_RS00385 (Emin_0076)	lpxC	80897..81730 (+)	834	WP_012414258.1	UDP-3-O-acyl-N-acetylglucosamine deacetylase	-
EMIN_RS00390 (Emin_0077)	-	81723..82199 (+)	477	WP_012414259.1	3-hydroxyacyl-ACP dehydratase FabZ family protein	-
EMIN_RS00395 (Emin_0078)	lpxA	82276..83079 (+)	804	WP_012414260.1	acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase	-
EMIN_RS00400 (Emin_0079)	-	83079..83900 (+)	822	WP_012414261.1	LpxI family protein	-
EMIN_RS00405 (Emin_0080)	-	83920..84588 (+)	669	WP_012414262.1	hypothetical protein	-
EMIN_RS09295	pilT	84711..85265 (-)	555	WP_202943288.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
EMIN_RS09300	-	85244..85780 (-)	537	WP_202943286.1	type IV pilus twitching motility protein PilT	-
EMIN_RS00415 (Emin_0082)	-	85825..87702 (-)	1878	WP_012414263.1	hypothetical protein	-
EMIN_RS00420 (Emin_0083)	gltX	87725..89191 (-)	1467	WP_012414264.1	glutamate--tRNA ligase	-

Sequence

Protein

• Length: 184 a.a.
• Molecular weight: 20608.60 Da
• Isoelectric Point: 6.3307

>NTDB_id=30810 EMIN_RS09295 WP_202943288.1 84711..85265(-) (pilT) [Elusimicrobium minutum Pei191]
MVNQREVGADTKDFGIALRHVLRQDPDVILVGEMRDFETIQAALNIAETGHLVFATLHTSDAAQSVNRIVDVFPPHQQEQ
ARMQLSFVLEAVFCQQLLPTADGKGRIMAAEVLLANSAARSIIRDKKSEQLINVMQTNRAAGMITMNQALGDLYMQKKIT
YQDAVFHCTDANDFKNYLQQQMSK

Nucleotide

• Length: 555 bp

>NTDB_id=30810 EMIN_RS09295 WP_202943288.1 84711..85265(-) (pilT) [Elusimicrobium minutum Pei191]
ATTGTTAACCAACGTGAAGTAGGCGCGGACACAAAGGATTTTGGCATAGCTTTAAGACACGTTCTGCGTCAAGACCCCGA
CGTTATTTTAGTAGGCGAAATGAGAGATTTTGAAACCATTCAAGCAGCCTTAAATATCGCCGAAACAGGCCACTTGGTTT
TTGCCACCCTTCACACGTCAGACGCGGCCCAAAGCGTTAACCGTATTGTTGACGTATTCCCGCCGCACCAGCAGGAACAG
GCTAGAATGCAGCTTTCTTTCGTTTTAGAAGCGGTTTTTTGCCAACAGCTTTTACCTACAGCGGATGGCAAAGGGCGTAT
TATGGCTGCGGAAGTTTTGCTTGCTAACTCGGCCGCCAGAAGTATTATAAGAGACAAAAAATCCGAGCAGCTTATCAACG
TAATGCAAACAAACAGAGCTGCGGGCATGATAACCATGAACCAAGCCTTGGGAGATTTATATATGCAAAAGAAAATCACC
TACCAAGACGCTGTTTTTCACTGCACGGACGCTAATGACTTTAAAAATTATCTGCAACAGCAAATGTCAAAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (2-76)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.322	94.565	0.533
pilT	Neisseria meningitidis 8013	54.268	89.13	0.484
pilT	Neisseria gonorrhoeae MS11	53.659	89.13	0.478
pilU	Pseudomonas stutzeri DSM 10701	47.429	95.109	0.451
pilU	Acinetobacter baylyi ADP1	44.565	100	0.446
pilT	Acinetobacter baylyi ADP1	48.171	89.13	0.429
pilT	Pseudomonas stutzeri DSM 10701	48.171	89.13	0.429
pilT	Legionella pneumophila strain ERS1305867	48.171	89.13	0.429
pilT	Acinetobacter baumannii D1279779	48.171	89.13	0.429
pilT	Acinetobacter baumannii strain A118	48.171	89.13	0.429
pilT	Acinetobacter nosocomialis M2	48.171	89.13	0.429
pilT	Legionella pneumophila strain Lp02	48.171	89.13	0.429
pilT	Vibrio cholerae strain A1552	53.378	80.435	0.429
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	53.378	80.435	0.429
pilT	Pseudomonas aeruginosa PAK	46.951	89.13	0.418
pilU	Vibrio cholerae strain A1552	42.541	98.37	0.418