Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DWG18_RS00310 Genome accession   NZ_CP031413
Coordinates   56013..57122 (-) Length   369 a.a.
NCBI ID   WP_115644538.1    Uniprot ID   A0A345ZGK6
Organism   Lysobacter sp. TY2-98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 51013..62122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWG18_RS00280 (DWG18_00280) rplM 52295..52723 (+) 429 WP_115644533.1 50S ribosomal protein L13 -
  DWG18_RS00285 (DWG18_00285) rpsI 52736..53128 (+) 393 WP_115644534.1 30S ribosomal protein S9 -
  DWG18_RS00295 (DWG18_00295) - 53368..54087 (+) 720 WP_205289377.1 2OG-Fe dioxygenase family protein -
  DWG18_RS00300 (DWG18_00300) - 54166..55152 (+) 987 WP_115644536.1 fumarylacetoacetate hydrolase family protein -
  DWG18_RS00305 (DWG18_00305) maiA 55236..55913 (+) 678 WP_115644537.1 maleylacetoacetate isomerase -
  DWG18_RS00310 (DWG18_00310) pilU 56013..57122 (-) 1110 WP_115644538.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DWG18_RS00315 (DWG18_00315) - 57216..58490 (+) 1275 WP_115644539.1 OmpA family protein -
  DWG18_RS00320 (DWG18_00320) - 58738..58956 (+) 219 WP_115644540.1 YdcH family protein -
  DWG18_RS00325 (DWG18_00325) - 59059..60435 (+) 1377 WP_115644541.1 pyridoxal-phosphate dependent enzyme -
  DWG18_RS00330 (DWG18_00330) - 60432..61346 (+) 915 WP_115644542.1 2OG-Fe(II) oxygenase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40795.01 Da        Isoelectric Point: 6.0911

>NTDB_id=306556 DWG18_RS00310 WP_115644538.1 56013..57122(-) (pilU) [Lysobacter sp. TY2-98]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLQPLGNTGLPSGMVKKIAYSLMDEGQVPEFETNLELNMAYALPDSGR
FRVNVFKQRGEVGMVIRAIKSVIPSIEELQLPACLKDIITSPRGLVLIVGSTGSGKSTTLAAMIDHRNTNMAGHILTIED
PIEFLHKHKKSIVNQREVGLDTHSFHNALKNAMREAPDVILIGEILDATTMEAAISFAETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVVGVDGRRLPATEVLINTPHVRDLMRRGQVHEIKQAMEESLEDGMETFDQCL
FRLQKEGRIEMEAALRAADSRDGLALKFRLSEGAGAEHDPYADMYSTTI

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=306556 DWG18_RS00310 WP_115644538.1 56013..57122(-) (pilU) [Lysobacter sp. TY2-98]
ATGGACATCGGCTACTTCCTGAAGCTGATGACGGAGAAGAATGCGTCGGACATGTTTCTGACGACGGGGGCTCCGGTCTA
CATCAAGGTGGAAGGCAAGCTCCAGCCGCTGGGCAATACGGGCCTGCCGTCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAAGGCCAGGTGCCGGAGTTCGAAACCAACCTCGAACTCAACATGGCGTATGCACTGCCGGACTCGGGACGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCAAGAGCGTGATCCCCTCGATCGA
GGAGCTGCAGCTTCCCGCATGCCTGAAGGACATCATCACCTCGCCGCGCGGCCTGGTGCTGATCGTCGGCAGTACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCATCGCAATACGAACATGGCCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTTCCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGCCTCGACACGCACAGCTTCCACAA
CGCGCTGAAGAATGCGATGCGCGAAGCGCCGGACGTCATCCTGATCGGCGAGATCCTTGACGCGACGACGATGGAAGCCG
CGATCTCGTTCGCGGAAACCGGCCATCTCTGCCTGGCGACGCTGCACTCGAACAACGCGGATCAGACGCTCGAGCGCATC
CTGAACTTTTTCCCGGAATCGGCGCACAAGAACGTGCTGATGAACCTCGCGCTCAACCTGCGCGCGGTGGTGAGCCAGCG
CCTCGTTGTCGGTGTCGACGGGCGCCGCCTGCCGGCGACCGAAGTGCTGATCAACACGCCGCACGTGCGTGACCTGATGC
GTCGCGGACAGGTACACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGACGGCATGGAGACGTTCGACCAGTGCCTG
TTCCGCCTGCAGAAGGAAGGTCGCATCGAGATGGAGGCCGCGCTGCGTGCCGCCGACTCGCGCGACGGCCTCGCGCTCAA
GTTCCGCCTCTCCGAGGGCGCGGGCGCCGAGCACGATCCGTACGCGGACATGTACAGCACCACGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345ZGK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

97.29

0.507
  pilU Acinetobacter baylyi ADP1

49.02

96.748

0.474
  pilU Vibrio cholerae strain A1552

49.153

95.935

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.027

98.916

0.455
  pilT Neisseria gonorrhoeae MS11

41.499

94.038

0.39
  pilT Neisseria meningitidis 8013

41.21

94.038

0.388
  pilT Acinetobacter baylyi ADP1

41.765

92.141

0.385
  pilT Acinetobacter baumannii strain A118

42.012

91.599

0.385
  pilT Acinetobacter nosocomialis M2

42.012

91.599

0.385
  pilT Acinetobacter baumannii D1279779

42.012

91.599

0.385
  pilT Pseudomonas aeruginosa PAK

42.012

91.599

0.385
  pilT Legionella pneumophila strain ERS1305867

41.42

91.599

0.379
  pilT Legionella pneumophila strain Lp02

41.42

91.599

0.379
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.599

0.379
  pilT Vibrio cholerae strain A1552

41.566

89.973

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.566

89.973

0.374

Multiple sequence alignment