Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Smn45_RS20700 Genome accession   NZ_CP031316
Coordinates   4304076..4305098 (-) Length   340 a.a.
NCBI ID   WP_115436520.1    Uniprot ID   -
Organism   Serratia marcescens strain N4-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4299076..4310098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Smn45_RS20670 (SmN45_4232) - 4299876..4300388 (+) 513 WP_115436517.1 SprT family zinc-dependent metalloprotease -
  Smn45_RS20675 (SmN45_4233) endA 4300490..4301185 (+) 696 WP_115436518.1 deoxyribonuclease I -
  Smn45_RS20680 (SmN45_4234) rsmE 4301255..4301986 (+) 732 WP_115436519.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Smn45_RS20685 (SmN45_4235) gshB 4301997..4302947 (+) 951 WP_016930084.1 glutathione synthase -
  Smn45_RS20690 (SmN45_4236) - 4303094..4303657 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  Smn45_RS20695 (SmN45_4237) ruvX 4303657..4304079 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  Smn45_RS20700 (SmN45_4238) pilT 4304076..4305098 (-) 1023 WP_115436520.1 type IV pilus twitching motility protein PilT Machinery gene
  Smn45_RS20705 (SmN45_4239) - 4305119..4305826 (+) 708 WP_115436521.1 YggS family pyridoxal phosphate-dependent enzyme -
  Smn45_RS20710 (SmN45_4240) proC 4305846..4306667 (+) 822 WP_079450221.1 pyrroline-5-carboxylate reductase -
  Smn45_RS20715 (SmN45_4241) - 4306699..4307253 (+) 555 WP_016930090.1 YggT family protein -
  Smn45_RS20720 (SmN45_4242) yggU 4307250..4307543 (+) 294 WP_115436522.1 DUF167 family protein YggU -
  Smn45_RS20725 (SmN45_4243) - 4307684..4308277 (+) 594 WP_016930092.1 XTP/dITP diphosphatase -
  Smn45_RS20730 (SmN45_4244) hemW 4308270..4309412 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  Smn45_RS20735 (SmN45_4245) - 4309449..4309697 (-) 249 WP_115436523.1 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36444.77 Da        Isoelectric Point: 7.5736

>NTDB_id=305525 Smn45_RS20700 WP_115436520.1 4304076..4305098(-) (pilT) [Serratia marcescens strain N4-5]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=305525 Smn45_RS20700 WP_115436520.1 4304076..4305098(-) (pilT) [Serratia marcescens strain N4-5]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTACGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCTGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCACCACAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Neisseria meningitidis 8013

50.294

100

0.503
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Pseudomonas aeruginosa PAK

49.267

100

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.704

99.412

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.673

99.706

0.485
  pilT Legionella pneumophila strain Lp02

48.529

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.529

100

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.034

100

0.456
  pilU Pseudomonas stutzeri DSM 10701

38.643

99.706

0.385
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382

Multiple sequence alignment