Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCE_RS02025 Genome accession   NC_010546
Coordinates   429557..430663 (-) Length   368 a.a.
NCBI ID   WP_009546507.1    Uniprot ID   B1WNC8
Organism   Crocosphaera subtropica ATCC 51142     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424557..435663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCE_RS02005 (cce_0415) - 425034..426770 (-) 1737 WP_009546503.1 NFACT RNA binding domain-containing protein -
  CCE_RS02010 (cce_0416) - 426845..427441 (-) 597 WP_009546504.1 hypothetical protein -
  CCE_RS02015 (cce_0417) - 427642..428214 (-) 573 WP_009546505.1 hypothetical protein -
  CCE_RS02020 (cce_0418) - 428303..429517 (-) 1215 WP_009546506.1 type II secretion system F family protein -
  CCE_RS02025 (cce_0419) pilT 429557..430663 (-) 1107 WP_009546507.1 type IV pilus twitching motility protein PilT Machinery gene
  CCE_RS02030 (cce_0420) pilB/pilB1 430700..432733 (-) 2034 WP_009546508.1 GspE/PulE family protein Machinery gene
  CCE_RS02035 (cce_0421) - 432814..433008 (-) 195 WP_009546509.1 hypothetical protein -
  CCE_RS02040 (cce_0422) kaiC 433248..434807 (-) 1560 WP_009546510.1 circadian clock protein KaiC -
  CCE_RS02045 (cce_0423) kaiB 434900..435214 (-) 315 WP_024750203.1 circadian clock protein KaiB -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40479.77 Da        Isoelectric Point: 7.3268

>NTDB_id=30458 CCE_RS02025 WP_009546507.1 429557..430663(-) (pilT) [Crocosphaera subtropica ATCC 51142]
MDMMIEDLMEQLVEMGGSDMHIQAGAPIYFRISGKLAPIDEEPLSPQESQKLIFSMLNNTQRKELEQNWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFEQLGLPNIVREMAERPRGLVLVTGQTGSGKTTTLAAILDLINRTRQEHILTV
EDPIEYVFPNVKSLFHQRQKGEDTKSFANALKASLREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTNSAAGTID
RIVDVFPANQQAQIRAMLSNSLIAVFSQCLAKKKNPKPGEFGRAMAQEIMIVTPAIANLMREGKAGQIYSAIQTGMKLGM
QTMEQALANLVVSGAVSFEEAISKSAKPDELQRLISGATAAVKGRVRR

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=30458 CCE_RS02025 WP_009546507.1 429557..430663(-) (pilT) [Crocosphaera subtropica ATCC 51142]
ATGGATATGATGATTGAAGACTTAATGGAACAACTTGTGGAAATGGGGGGTTCCGATATGCACATTCAAGCCGGTGCGCC
GATTTATTTTCGCATTAGTGGGAAACTAGCCCCCATTGATGAAGAACCCCTTTCTCCTCAAGAAAGTCAAAAATTAATTT
TCAGTATGCTCAATAATACTCAACGAAAGGAGTTAGAACAAAACTGGGAATTAGACTGTTCCTATGGGGTTAAAGGGTTG
GCACGATTTCGGGTGAATGTTTATAAAGAACGGGGGTGTTATGCTGCTTGTTTACGGGCTTTATCTTCTAAAATTCCTAA
TTTTGAACAGTTAGGTCTGCCCAATATTGTACGAGAAATGGCTGAACGACCACGGGGTTTGGTGTTGGTGACGGGACAAA
CCGGATCGGGTAAAACCACCACCTTAGCAGCGATTTTAGACTTAATTAATCGTACTCGACAAGAGCATATTTTAACGGTT
GAAGATCCGATTGAATACGTTTTTCCTAATGTTAAAAGCCTCTTTCATCAACGACAAAAAGGAGAAGACACCAAGTCCTT
TGCCAATGCCTTAAAAGCCTCCTTACGGGAAGATCCGGACATCATTTTAGTTGGGGAAATGCGAGACTTAGAAACCATTA
GTTTAGCCATCACCGCAGCAGAAACAGGACACTTAGTTTTTGGAACGTTACACACCAATTCAGCAGCCGGTACCATTGAC
CGTATTGTTGACGTATTTCCTGCTAATCAACAGGCTCAAATTCGGGCGATGTTATCTAACTCTTTAATCGCTGTTTTTAG
TCAATGTTTAGCGAAGAAAAAGAACCCGAAGCCTGGTGAATTTGGTCGGGCGATGGCGCAAGAAATTATGATTGTAACCC
CTGCCATTGCTAACCTAATGCGAGAAGGGAAAGCTGGTCAAATTTATTCGGCGATCCAAACAGGAATGAAACTGGGAATG
CAAACGATGGAACAAGCCTTAGCTAACTTGGTTGTTAGCGGAGCAGTTTCCTTTGAAGAAGCCATTTCTAAAAGTGCTAA
ACCCGATGAGTTACAACGATTAATTAGTGGAGCAACTGCTGCGGTTAAGGGTAGAGTGCGTCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1WNC8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.602

94.293

0.505
  pilT Legionella pneumophila strain ERS1305867

53.602

94.293

0.505
  pilT Pseudomonas aeruginosa PAK

52.149

94.837

0.495
  pilT Acinetobacter baylyi ADP1

51.289

94.837

0.486
  pilT Acinetobacter baumannii D1279779

51.003

94.837

0.484
  pilT Acinetobacter baumannii strain A118

51.003

94.837

0.484
  pilT Acinetobacter nosocomialis M2

51.003

94.837

0.484
  pilT Pseudomonas stutzeri DSM 10701

50.716

94.837

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.729

93.207

0.473
  pilT Vibrio cholerae strain A1552

50.729

93.207

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.5

97.826

0.465
  pilT Neisseria gonorrhoeae MS11

50.147

92.12

0.462
  pilT Neisseria meningitidis 8013

50.147

92.12

0.462
  pilU Pseudomonas stutzeri DSM 10701

38.81

95.924

0.372
  pilU Vibrio cholerae strain A1552

37.96

95.924

0.364

Multiple sequence alignment