Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CCE_RS02030 Genome accession   NC_010546
Coordinates   430700..432733 (-) Length   677 a.a.
NCBI ID   WP_009546508.1    Uniprot ID   B1WNC9
Organism   Crocosphaera subtropica ATCC 51142     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425700..437733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCE_RS02010 (cce_0416) - 426845..427441 (-) 597 WP_009546504.1 hypothetical protein -
  CCE_RS02015 (cce_0417) - 427642..428214 (-) 573 WP_009546505.1 hypothetical protein -
  CCE_RS02020 (cce_0418) - 428303..429517 (-) 1215 WP_009546506.1 type II secretion system F family protein -
  CCE_RS02025 (cce_0419) pilT 429557..430663 (-) 1107 WP_009546507.1 type IV pilus twitching motility protein PilT Machinery gene
  CCE_RS02030 (cce_0420) pilB/pilB1 430700..432733 (-) 2034 WP_009546508.1 GspE/PulE family protein Machinery gene
  CCE_RS02035 (cce_0421) - 432814..433008 (-) 195 WP_009546509.1 hypothetical protein -
  CCE_RS02040 (cce_0422) kaiC 433248..434807 (-) 1560 WP_009546510.1 circadian clock protein KaiC -
  CCE_RS02045 (cce_0423) kaiB 434900..435214 (-) 315 WP_024750203.1 circadian clock protein KaiB -
  CCE_RS02050 (cce_0424) - 435307..436203 (-) 897 WP_009546512.1 circadian clock protein KaiA -
  CCE_RS02055 (cce_0425) - 436319..437236 (-) 918 WP_009546513.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 677 a.a.        Molecular weight: 75252.84 Da        Isoelectric Point: 5.3168

>NTDB_id=30459 CCE_RS02030 WP_009546508.1 430700..432733(-) (pilB/pilB1) [Crocosphaera subtropica ATCC 51142]
MSSLSSSNLSSSKRSRAVALRNYFSPFGNKLIEAGYVGPDQMKQALVETRKSGRPLTEVLQVMTGEPLSPDLLRQYKKHH
LFELKILYGVDSIDPEVSPVADRQMAELINSLISFDICRRHRILPLSQHGGEPPSVLVAMVDPDNLAAQDDLNRILRAKN
LELRRMVITQEDYDRLLEQYHELQGEIDAENERLKQEAAMNKMADLTAIVGNLDASISDAEDESHDALDSNDADQAPIIN
LVNKILAKALQEGTSDIHVEPQEETLRIRFRKDGVLQQAFDPLPRKVTPAVVARFKIMADLDIAERRLPQDGKIRRIYQG
RKVDFRVNTLPSRYGEKVCLRILDNSATQLGLDKLITNQETLQIVRDLASRPFGLFLVTGPTGSGKSTSLYSVLAERNNP
GVNISTAEDPIEYSLPGITQVQVIREKGMNFASILRAFMRQDPDVILVGETRDVETAKTAIEAALTGHLVLTTLHTNDAP
GAVARLDEMGVEPFMISGALLGVLAQRLMRRVCSECRVAYHPTKAELARFGLSASNDDEMTIYKANKLTADEITEAKQNG
TLCEKCGGSGYKGRVGVYEVMRNTERIQSLINEGATTDRIKEAAVEEGMITILAYSLQLVQEGHTTLEEVERVTFTDTGL
EAELKAKRKSSLECKTCHAGLEPEWMDCPYCMTPRFT

Nucleotide


Download         Length: 2034 bp        

>NTDB_id=30459 CCE_RS02030 WP_009546508.1 430700..432733(-) (pilB/pilB1) [Crocosphaera subtropica ATCC 51142]
ATGTCCTCTTTGTCTTCTTCCAATTTGTCTTCTTCCAAACGCAGTCGGGCGGTTGCCCTGCGTAATTACTTTTCACCCTT
TGGCAACAAACTGATCGAAGCGGGTTATGTAGGCCCGGATCAGATGAAACAAGCCCTAGTCGAAACCCGTAAAAGCGGTC
GTCCTTTAACTGAAGTGTTACAGGTGATGACTGGGGAACCCTTATCCCCCGATTTATTACGACAATATAAAAAACATCAT
TTATTTGAACTGAAAATCCTTTATGGTGTCGACTCTATCGATCCCGAAGTCTCTCCTGTCGCGGATCGCCAGATGGCTGA
ATTAATCAATTCATTAATTTCTTTTGATATTTGTCGTCGCCATAGAATTTTACCTTTATCTCAACATGGCGGGGAACCCC
CTTCCGTGTTAGTGGCGATGGTTGACCCCGATAACCTAGCAGCCCAAGATGACTTAAACCGTATTTTGAGGGCGAAAAAC
CTAGAGTTACGGCGCATGGTCATTACCCAAGAAGATTATGATCGCTTATTAGAACAGTATCATGAACTTCAAGGGGAGAT
TGATGCAGAAAATGAACGTCTCAAACAAGAGGCGGCCATGAATAAAATGGCTGATCTGACAGCCATCGTTGGTAACCTAG
ATGCCAGCATCTCTGATGCCGAAGATGAAAGTCACGATGCCTTAGACTCCAATGATGCTGATCAAGCACCCATTATTAAC
TTAGTCAATAAAATCCTCGCCAAAGCTCTCCAAGAAGGCACTTCCGACATTCATGTTGAACCCCAAGAAGAAACCTTAAG
AATTCGTTTCCGTAAAGATGGGGTACTGCAACAAGCCTTTGATCCCCTACCCCGAAAAGTCACCCCGGCGGTAGTAGCAC
GGTTTAAAATTATGGCGGACCTCGATATTGCCGAACGTCGTCTTCCTCAAGATGGTAAGATTCGACGCATTTATCAAGGT
CGAAAAGTGGATTTTCGGGTTAATACCTTACCCAGTCGGTATGGAGAAAAAGTCTGTTTACGGATCTTAGATAACTCGGC
CACTCAATTAGGCTTAGATAAACTCATTACCAACCAAGAAACCTTACAAATTGTACGAGACTTGGCGAGTCGTCCGTTTG
GCCTCTTTCTCGTCACTGGACCAACGGGTTCGGGTAAATCTACCAGTTTGTATTCTGTCTTAGCTGAAAGAAATAATCCG
GGGGTCAATATTAGCACTGCCGAAGATCCTATCGAATATTCTTTACCCGGTATTACTCAGGTGCAGGTGATTCGAGAAAA
AGGCATGAACTTCGCCTCTATTTTACGGGCATTCATGCGACAAGATCCCGACGTTATCCTGGTGGGGGAAACCAGGGACG
TAGAAACAGCAAAAACCGCTATTGAAGCAGCCTTAACAGGACACTTAGTTTTAACCACCCTACACACCAACGATGCACCA
GGAGCGGTTGCTCGTCTCGATGAAATGGGGGTTGAACCCTTTATGATTTCTGGAGCGTTATTAGGGGTGTTAGCGCAACG
GTTAATGCGTCGGGTATGTAGTGAATGTCGTGTTGCTTATCATCCTACTAAAGCCGAATTAGCCCGTTTTGGCTTGTCGG
CTTCTAATGATGATGAAATGACTATTTATAAGGCGAATAAATTAACCGCAGACGAAATTACTGAAGCCAAACAAAATGGT
ACCCTGTGCGAAAAATGTGGCGGTAGTGGCTATAAGGGACGGGTTGGGGTTTATGAAGTGATGCGTAATACCGAACGGAT
TCAAAGCCTTATTAACGAAGGAGCAACCACTGATCGCATTAAGGAAGCAGCTGTCGAAGAAGGAATGATCACCATCCTTG
CTTACAGTTTACAACTGGTTCAAGAAGGTCATACCACCCTTGAAGAAGTGGAACGGGTGACGTTTACAGATACGGGACTC
GAAGCAGAATTAAAAGCCAAGCGCAAGAGTTCCCTTGAGTGTAAAACCTGTCATGCAGGACTTGAACCGGAGTGGATGGA
CTGTCCCTATTGTATGACTCCTCGATTTACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(244-511)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1WNC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

76.586

97.784

0.749
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.065

90.694

0.363

Multiple sequence alignment